Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25100 and CA265_RS25105 are separated by 170 nucleotides CA265_RS25105 and CA265_RS25110 are separated by 291 nucleotides
CA265_RS25100: CA265_RS25100 - hypothetical protein, at 6,112,140 to 6,113,105
_RS25100
CA265_RS25105: CA265_RS25105 - alpha/beta hydrolase, at 6,113,276 to 6,114,268
_RS25105
CA265_RS25110: CA265_RS25110 - polyprenyl synthetase, at 6,114,560 to 6,115,531
_RS25110
Position (kb)
6113
6114
6115 Strain fitness (log2 ratio)
-1
0
1
2 at 6112.286 kb on - strand, within CA265_RS25100 at 6112.383 kb on + strand, within CA265_RS25100 at 6112.414 kb on + strand, within CA265_RS25100 at 6112.497 kb on + strand, within CA265_RS25100 at 6112.497 kb on + strand, within CA265_RS25100 at 6112.498 kb on - strand, within CA265_RS25100 at 6112.571 kb on + strand, within CA265_RS25100 at 6112.572 kb on - strand, within CA265_RS25100 at 6112.593 kb on + strand, within CA265_RS25100 at 6112.609 kb on + strand, within CA265_RS25100 at 6112.609 kb on + strand, within CA265_RS25100 at 6112.696 kb on + strand, within CA265_RS25100 at 6112.740 kb on + strand, within CA265_RS25100 at 6112.773 kb on - strand, within CA265_RS25100 at 6112.917 kb on - strand, within CA265_RS25100 at 6112.922 kb on - strand, within CA265_RS25100 at 6112.966 kb on - strand, within CA265_RS25100 at 6113.004 kb on + strand, within CA265_RS25100 at 6113.161 kb on + strand at 6113.162 kb on - strand at 6113.168 kb on + strand at 6113.218 kb on - strand at 6113.219 kb on + strand at 6113.268 kb on - strand at 6113.272 kb on - strand at 6113.272 kb on - strand at 6113.320 kb on + strand at 6113.353 kb on + strand at 6113.356 kb on + strand at 6113.357 kb on - strand at 6113.357 kb on - strand at 6113.358 kb on + strand at 6113.359 kb on - strand at 6113.370 kb on - strand at 6113.408 kb on + strand, within CA265_RS25105 at 6113.466 kb on - strand at 6113.499 kb on + strand, within CA265_RS25105 at 6113.499 kb on + strand, within CA265_RS25105 at 6113.510 kb on + strand, within CA265_RS25105 at 6113.522 kb on + strand, within CA265_RS25105 at 6113.543 kb on + strand, within CA265_RS25105 at 6113.549 kb on - strand, within CA265_RS25105 at 6113.640 kb on - strand, within CA265_RS25105 at 6113.644 kb on + strand, within CA265_RS25105 at 6113.813 kb on - strand, within CA265_RS25105 at 6113.813 kb on - strand, within CA265_RS25105 at 6113.856 kb on + strand, within CA265_RS25105 at 6113.917 kb on + strand, within CA265_RS25105 at 6113.972 kb on - strand, within CA265_RS25105 at 6114.030 kb on + strand, within CA265_RS25105 at 6114.031 kb on - strand, within CA265_RS25105 at 6114.132 kb on + strand, within CA265_RS25105 at 6114.132 kb on + strand, within CA265_RS25105 at 6114.213 kb on + strand at 6114.228 kb on - strand at 6114.244 kb on + strand at 6114.249 kb on - strand at 6114.256 kb on - strand at 6114.313 kb on + strand at 6114.336 kb on + strand at 6114.376 kb on + strand at 6114.471 kb on + strand at 6114.502 kb on - strand at 6114.527 kb on - strand at 6114.559 kb on - strand at 6114.589 kb on + strand at 6114.674 kb on + strand, within CA265_RS25110 at 6114.677 kb on - strand, within CA265_RS25110 at 6114.677 kb on - strand, within CA265_RS25110 at 6114.677 kb on - strand, within CA265_RS25110 at 6114.789 kb on + strand, within CA265_RS25110 at 6114.823 kb on - strand, within CA265_RS25110 at 6114.857 kb on - strand, within CA265_RS25110 at 6114.903 kb on - strand, within CA265_RS25110 at 6115.012 kb on - strand, within CA265_RS25110 at 6115.083 kb on + strand, within CA265_RS25110 at 6115.140 kb on + strand, within CA265_RS25110 at 6115.199 kb on - strand, within CA265_RS25110 at 6115.225 kb on - strand, within CA265_RS25110 at 6115.229 kb on - strand, within CA265_RS25110
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 6,112,286 - CA265_RS25100 0.15 +0.4 6,112,383 + CA265_RS25100 0.25 +0.8 6,112,414 + CA265_RS25100 0.28 +0.1 6,112,497 + CA265_RS25100 0.37 +0.4 6,112,497 + CA265_RS25100 0.37 +0.2 6,112,498 - CA265_RS25100 0.37 -1.5 6,112,571 + CA265_RS25100 0.45 -1.6 6,112,572 - CA265_RS25100 0.45 -0.2 6,112,593 + CA265_RS25100 0.47 +0.2 6,112,609 + CA265_RS25100 0.49 -0.3 6,112,609 + CA265_RS25100 0.49 +0.3 6,112,696 + CA265_RS25100 0.58 +1.3 6,112,740 + CA265_RS25100 0.62 -0.3 6,112,773 - CA265_RS25100 0.66 +0.9 6,112,917 - CA265_RS25100 0.80 +0.0 6,112,922 - CA265_RS25100 0.81 +0.2 6,112,966 - CA265_RS25100 0.86 +0.6 6,113,004 + CA265_RS25100 0.89 -1.1 6,113,161 + +0.4 6,113,162 - -1.5 6,113,168 + -0.4 6,113,218 - -0.0 6,113,219 + -0.4 6,113,268 - +0.5 6,113,272 - +0.2 6,113,272 - -0.9 6,113,320 + +0.6 6,113,353 + +0.2 6,113,356 + -0.4 6,113,357 - +0.0 6,113,357 - +0.6 6,113,358 + -0.9 6,113,359 - +0.0 6,113,370 - +0.4 6,113,408 + CA265_RS25105 0.13 -1.2 6,113,466 - +0.5 6,113,499 + CA265_RS25105 0.22 -0.7 6,113,499 + CA265_RS25105 0.22 -0.3 6,113,510 + CA265_RS25105 0.24 -0.8 6,113,522 + CA265_RS25105 0.25 -0.3 6,113,543 + CA265_RS25105 0.27 -0.3 6,113,549 - CA265_RS25105 0.27 -0.4 6,113,640 - CA265_RS25105 0.37 -0.7 6,113,644 + CA265_RS25105 0.37 +0.1 6,113,813 - CA265_RS25105 0.54 +0.0 6,113,813 - CA265_RS25105 0.54 -0.6 6,113,856 + CA265_RS25105 0.58 -1.3 6,113,917 + CA265_RS25105 0.65 +0.1 6,113,972 - CA265_RS25105 0.70 -0.5 6,114,030 + CA265_RS25105 0.76 -1.4 6,114,031 - CA265_RS25105 0.76 -0.3 6,114,132 + CA265_RS25105 0.86 -1.4 6,114,132 + CA265_RS25105 0.86 -0.5 6,114,213 + -0.8 6,114,228 - -0.4 6,114,244 + -0.8 6,114,249 - +0.4 6,114,256 - -0.7 6,114,313 + +0.6 6,114,336 + +0.7 6,114,376 + -1.5 6,114,471 + +0.0 6,114,502 - +0.4 6,114,527 - -0.2 6,114,559 - -0.0 6,114,589 + +2.0 6,114,674 + CA265_RS25110 0.12 +0.9 6,114,677 - CA265_RS25110 0.12 +0.3 6,114,677 - CA265_RS25110 0.12 +0.9 6,114,677 - CA265_RS25110 0.12 +0.3 6,114,789 + CA265_RS25110 0.24 -0.8 6,114,823 - CA265_RS25110 0.27 +0.5 6,114,857 - CA265_RS25110 0.31 +1.5 6,114,903 - CA265_RS25110 0.35 +0.8 6,115,012 - CA265_RS25110 0.47 +2.1 6,115,083 + CA265_RS25110 0.54 +0.2 6,115,140 + CA265_RS25110 0.60 +2.1 6,115,199 - CA265_RS25110 0.66 +0.9 6,115,225 - CA265_RS25110 0.68 +1.0 6,115,229 - CA265_RS25110 0.69 +1.4
Or see this region's nucleotide sequence