Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23220 and CA265_RS23225 are separated by 11 nucleotides CA265_RS23225 and CA265_RS23230 are separated by 669 nucleotides CA265_RS23230 and CA265_RS23235 are separated by 96 nucleotides
CA265_RS23220: CA265_RS23220 - 12-oxophytodienoate reductase, at 5,679,346 to 5,680,446
_RS23220
CA265_RS23225: CA265_RS23225 - transcriptional regulator, at 5,680,458 to 5,680,760
_RS23225
CA265_RS23230: CA265_RS23230 - hypothetical protein, at 5,681,430 to 5,681,660
_RS23230
CA265_RS23235: CA265_RS23235 - transcriptional regulator, at 5,681,757 to 5,682,155
_RS23235
Position (kb) 5680
5681 Strain fitness (log2 ratio) -1
0
1
2 at 5679.458 kb on - strand, within CA265_RS23220 at 5679.510 kb on + strand, within CA265_RS23220 at 5679.775 kb on + strand, within CA265_RS23220 at 5679.783 kb on + strand, within CA265_RS23220 at 5679.787 kb on + strand, within CA265_RS23220 at 5679.891 kb on + strand, within CA265_RS23220 at 5679.897 kb on + strand, within CA265_RS23220 at 5679.926 kb on + strand, within CA265_RS23220 at 5679.944 kb on - strand, within CA265_RS23220 at 5680.122 kb on + strand, within CA265_RS23220 at 5680.155 kb on + strand, within CA265_RS23220 at 5680.172 kb on - strand, within CA265_RS23220 at 5680.285 kb on + strand, within CA265_RS23220 at 5680.430 kb on - strand at 5680.434 kb on + strand at 5680.435 kb on - strand at 5680.533 kb on + strand, within CA265_RS23225 at 5680.566 kb on + strand, within CA265_RS23225 at 5680.567 kb on - strand, within CA265_RS23225 at 5680.579 kb on + strand, within CA265_RS23225 at 5680.579 kb on + strand, within CA265_RS23225 at 5680.695 kb on + strand, within CA265_RS23225 at 5680.813 kb on + strand at 5680.825 kb on + strand at 5680.838 kb on + strand at 5680.839 kb on - strand at 5681.075 kb on - strand at 5681.406 kb on + strand at 5681.461 kb on + strand, within CA265_RS23230 at 5681.461 kb on + strand, within CA265_RS23230 at 5681.463 kb on + strand, within CA265_RS23230 at 5681.480 kb on + strand at 5681.480 kb on + strand, within CA265_RS23230 at 5681.558 kb on - strand, within CA265_RS23230 at 5681.645 kb on + strand at 5681.646 kb on - strand at 5681.653 kb on - strand at 5681.735 kb on + strand at 5681.758 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 5,679,458 - CA265_RS23220 0.10 +0.8 5,679,510 + CA265_RS23220 0.15 -0.8 5,679,775 + CA265_RS23220 0.39 -0.1 5,679,783 + CA265_RS23220 0.40 -0.6 5,679,787 + CA265_RS23220 0.40 -1.1 5,679,891 + CA265_RS23220 0.50 -0.4 5,679,897 + CA265_RS23220 0.50 +2.6 5,679,926 + CA265_RS23220 0.53 +0.4 5,679,944 - CA265_RS23220 0.54 +1.3 5,680,122 + CA265_RS23220 0.70 +0.2 5,680,155 + CA265_RS23220 0.73 -0.4 5,680,172 - CA265_RS23220 0.75 -1.5 5,680,285 + CA265_RS23220 0.85 -1.4 5,680,430 - +0.2 5,680,434 + -0.4 5,680,435 - +1.0 5,680,533 + CA265_RS23225 0.25 +0.0 5,680,566 + CA265_RS23225 0.36 -1.0 5,680,567 - CA265_RS23225 0.36 +0.2 5,680,579 + CA265_RS23225 0.40 -0.0 5,680,579 + CA265_RS23225 0.40 -1.4 5,680,695 + CA265_RS23225 0.78 -0.8 5,680,813 + -0.2 5,680,825 + -0.1 5,680,838 + -0.1 5,680,839 - -0.6 5,681,075 - +1.3 5,681,406 + +0.1 5,681,461 + CA265_RS23230 0.13 -0.6 5,681,461 + CA265_RS23230 0.13 -0.4 5,681,463 + CA265_RS23230 0.14 +0.3 5,681,480 + +0.6 5,681,480 + CA265_RS23230 0.22 +0.4 5,681,558 - CA265_RS23230 0.55 -0.2 5,681,645 + +0.0 5,681,646 - -0.3 5,681,653 - +0.3 5,681,735 + +0.0 5,681,758 + +0.6
Or see this region's nucleotide sequence