Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20715 and CA265_RS20720 are separated by 5 nucleotides CA265_RS20720 and CA265_RS20725 overlap by 4 nucleotides CA265_RS20725 and CA265_RS20730 overlap by 4 nucleotides CA265_RS20730 and CA265_RS20735 are separated by 5 nucleotides
CA265_RS20715: CA265_RS20715 - conjugal transfer protein TraG, at 5,119,680 to 5,122,130
_RS20715
CA265_RS20720: CA265_RS20720 - hypothetical protein, at 5,122,136 to 5,122,798
_RS20720
CA265_RS20725: CA265_RS20725 - hypothetical protein, at 5,122,795 to 5,123,451
_RS20725
CA265_RS20730: CA265_RS20730 - hypothetical protein, at 5,123,448 to 5,124,113
_RS20730
CA265_RS20735: CA265_RS20735 - hypothetical protein, at 5,124,119 to 5,124,898
_RS20735
Position (kb)
5122
5123
5124 Strain fitness (log2 ratio)
-1
0
1
2
3 at 5121.903 kb on + strand at 5121.931 kb on + strand at 5121.933 kb on + strand at 5121.934 kb on - strand at 5121.955 kb on - strand at 5122.009 kb on - strand at 5122.119 kb on - strand at 5122.132 kb on + strand at 5122.135 kb on + strand at 5122.154 kb on - strand at 5122.154 kb on - strand at 5122.200 kb on - strand at 5122.234 kb on - strand, within CA265_RS20720 at 5122.355 kb on + strand, within CA265_RS20720 at 5122.356 kb on - strand, within CA265_RS20720 at 5122.356 kb on - strand, within CA265_RS20720 at 5122.356 kb on - strand, within CA265_RS20720 at 5122.357 kb on + strand, within CA265_RS20720 at 5122.415 kb on + strand, within CA265_RS20720 at 5122.416 kb on - strand, within CA265_RS20720 at 5122.433 kb on - strand, within CA265_RS20720 at 5122.433 kb on - strand, within CA265_RS20720 at 5122.434 kb on + strand, within CA265_RS20720 at 5122.435 kb on - strand, within CA265_RS20720 at 5122.545 kb on + strand, within CA265_RS20720 at 5122.670 kb on + strand, within CA265_RS20720 at 5122.670 kb on + strand, within CA265_RS20720 at 5122.682 kb on + strand, within CA265_RS20720 at 5122.682 kb on + strand, within CA265_RS20720 at 5122.683 kb on - strand, within CA265_RS20720 at 5122.736 kb on - strand at 5122.747 kb on + strand at 5122.747 kb on + strand at 5122.809 kb on + strand at 5122.810 kb on - strand at 5122.810 kb on - strand at 5122.815 kb on - strand at 5122.835 kb on + strand at 5123.079 kb on - strand, within CA265_RS20725 at 5123.089 kb on - strand, within CA265_RS20725 at 5123.121 kb on + strand, within CA265_RS20725 at 5123.122 kb on - strand, within CA265_RS20725 at 5123.122 kb on - strand, within CA265_RS20725 at 5123.187 kb on + strand, within CA265_RS20725 at 5123.187 kb on + strand, within CA265_RS20725 at 5123.201 kb on - strand at 5123.256 kb on + strand, within CA265_RS20725 at 5123.292 kb on + strand, within CA265_RS20725 at 5123.463 kb on - strand at 5123.463 kb on - strand at 5123.468 kb on - strand at 5123.477 kb on - strand at 5123.593 kb on - strand, within CA265_RS20730 at 5123.601 kb on - strand, within CA265_RS20730 at 5123.624 kb on + strand, within CA265_RS20730 at 5123.625 kb on - strand, within CA265_RS20730 at 5123.702 kb on - strand, within CA265_RS20730 at 5123.748 kb on - strand, within CA265_RS20730 at 5123.748 kb on - strand, within CA265_RS20730 at 5123.750 kb on - strand, within CA265_RS20730 at 5123.750 kb on - strand, within CA265_RS20730 at 5123.822 kb on - strand, within CA265_RS20730 at 5124.048 kb on + strand at 5124.100 kb on + strand at 5124.100 kb on + strand at 5124.112 kb on - strand at 5124.119 kb on - strand at 5124.147 kb on + strand at 5124.199 kb on + strand, within CA265_RS20735 at 5124.199 kb on + strand, within CA265_RS20735 at 5124.199 kb on + strand, within CA265_RS20735 at 5124.273 kb on + strand, within CA265_RS20735 at 5124.329 kb on - strand, within CA265_RS20735 at 5124.353 kb on - strand, within CA265_RS20735 at 5124.354 kb on + strand, within CA265_RS20735 at 5124.354 kb on + strand, within CA265_RS20735 at 5124.354 kb on + strand, within CA265_RS20735 at 5124.354 kb on + strand, within CA265_RS20735 at 5124.355 kb on - strand, within CA265_RS20735 at 5124.381 kb on + strand, within CA265_RS20735 at 5124.400 kb on + strand, within CA265_RS20735 at 5124.406 kb on + strand, within CA265_RS20735 at 5124.407 kb on - strand, within CA265_RS20735 at 5124.415 kb on + strand, within CA265_RS20735 at 5124.416 kb on - strand, within CA265_RS20735 at 5124.448 kb on + strand, within CA265_RS20735 at 5124.449 kb on - strand, within CA265_RS20735 at 5124.449 kb on - strand, within CA265_RS20735
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 5,121,903 + +0.6 5,121,931 + -0.0 5,121,933 + -0.2 5,121,934 - -0.2 5,121,955 - +0.4 5,122,009 - +0.2 5,122,119 - +0.1 5,122,132 + -0.5 5,122,135 + -0.9 5,122,154 - -0.0 5,122,154 - +0.3 5,122,200 - +0.0 5,122,234 - CA265_RS20720 0.15 +0.8 5,122,355 + CA265_RS20720 0.33 -0.7 5,122,356 - CA265_RS20720 0.33 -1.0 5,122,356 - CA265_RS20720 0.33 +1.3 5,122,356 - CA265_RS20720 0.33 +0.4 5,122,357 + CA265_RS20720 0.33 -0.4 5,122,415 + CA265_RS20720 0.42 -0.3 5,122,416 - CA265_RS20720 0.42 +2.3 5,122,433 - CA265_RS20720 0.45 -0.1 5,122,433 - CA265_RS20720 0.45 +1.0 5,122,434 + CA265_RS20720 0.45 +0.4 5,122,435 - CA265_RS20720 0.45 +0.4 5,122,545 + CA265_RS20720 0.62 -0.9 5,122,670 + CA265_RS20720 0.81 +1.0 5,122,670 + CA265_RS20720 0.81 +0.8 5,122,682 + CA265_RS20720 0.82 +0.0 5,122,682 + CA265_RS20720 0.82 +0.0 5,122,683 - CA265_RS20720 0.83 +0.7 5,122,736 - -0.3 5,122,747 + +1.0 5,122,747 + +0.0 5,122,809 + -1.3 5,122,810 - -0.2 5,122,810 - +0.0 5,122,815 - +0.9 5,122,835 + -0.4 5,123,079 - CA265_RS20725 0.43 +0.1 5,123,089 - CA265_RS20725 0.45 -0.8 5,123,121 + CA265_RS20725 0.50 +0.8 5,123,122 - CA265_RS20725 0.50 +0.7 5,123,122 - CA265_RS20725 0.50 +1.5 5,123,187 + CA265_RS20725 0.60 -0.4 5,123,187 + CA265_RS20725 0.60 -0.1 5,123,201 - +3.6 5,123,256 + CA265_RS20725 0.70 -0.7 5,123,292 + CA265_RS20725 0.76 +0.8 5,123,463 - +0.1 5,123,463 - +0.2 5,123,468 - -0.9 5,123,477 - +0.1 5,123,593 - CA265_RS20730 0.22 -0.0 5,123,601 - CA265_RS20730 0.23 +0.4 5,123,624 + CA265_RS20730 0.26 -0.8 5,123,625 - CA265_RS20730 0.27 +0.1 5,123,702 - CA265_RS20730 0.38 +0.6 5,123,748 - CA265_RS20730 0.45 +1.4 5,123,748 - CA265_RS20730 0.45 -0.1 5,123,750 - CA265_RS20730 0.45 +0.6 5,123,750 - CA265_RS20730 0.45 -0.4 5,123,822 - CA265_RS20730 0.56 +0.4 5,124,048 + -0.5 5,124,100 + -0.3 5,124,100 + +0.9 5,124,112 - -1.7 5,124,119 - +0.7 5,124,147 + +0.2 5,124,199 + CA265_RS20735 0.10 +1.1 5,124,199 + CA265_RS20735 0.10 -0.3 5,124,199 + CA265_RS20735 0.10 -0.4 5,124,273 + CA265_RS20735 0.20 -0.6 5,124,329 - CA265_RS20735 0.27 +0.1 5,124,353 - CA265_RS20735 0.30 +0.4 5,124,354 + CA265_RS20735 0.30 -0.7 5,124,354 + CA265_RS20735 0.30 +2.2 5,124,354 + CA265_RS20735 0.30 -0.5 5,124,354 + CA265_RS20735 0.30 +0.3 5,124,355 - CA265_RS20735 0.30 +0.2 5,124,381 + CA265_RS20735 0.34 -0.4 5,124,400 + CA265_RS20735 0.36 +1.2 5,124,406 + CA265_RS20735 0.37 -0.1 5,124,407 - CA265_RS20735 0.37 -0.5 5,124,415 + CA265_RS20735 0.38 +0.5 5,124,416 - CA265_RS20735 0.38 +1.0 5,124,448 + CA265_RS20735 0.42 +0.2 5,124,449 - CA265_RS20735 0.42 -0.5 5,124,449 - CA265_RS20735 0.42 -0.1
Or see this region's nucleotide sequence