Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20000 and CA265_RS20005 are separated by 167 nucleotides CA265_RS20005 and CA265_RS20010 are separated by 3 nucleotides CA265_RS20010 and CA265_RS20015 are separated by 116 nucleotides
CA265_RS20000: CA265_RS20000 - sigma-54-dependent Fis family transcriptional regulator, at 4,945,313 to 4,946,722
_RS20000
CA265_RS20005: CA265_RS20005 - enoyl-CoA hydratase, at 4,946,890 to 4,947,669
_RS20005
CA265_RS20010: CA265_RS20010 - methylmalonyl-CoA mutase, at 4,947,673 to 4,948,092
_RS20010
CA265_RS20015: CA265_RS20015 - glutamine--tRNA ligase, at 4,948,209 to 4,949,864
_RS20015
Position (kb)
4947
4948
4949 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4946.704 kb on + strand at 4946.704 kb on + strand at 4946.708 kb on + strand at 4946.709 kb on - strand at 4946.741 kb on + strand at 4946.800 kb on - strand at 4946.811 kb on - strand at 4946.891 kb on + strand at 4946.992 kb on - strand, within CA265_RS20005 at 4947.009 kb on + strand, within CA265_RS20005 at 4947.009 kb on + strand, within CA265_RS20005 at 4947.130 kb on + strand, within CA265_RS20005 at 4947.168 kb on + strand at 4947.191 kb on - strand, within CA265_RS20005 at 4947.199 kb on - strand, within CA265_RS20005 at 4947.261 kb on + strand, within CA265_RS20005 at 4947.261 kb on + strand, within CA265_RS20005 at 4947.261 kb on + strand, within CA265_RS20005 at 4947.304 kb on + strand, within CA265_RS20005 at 4947.305 kb on - strand, within CA265_RS20005 at 4947.305 kb on - strand, within CA265_RS20005 at 4947.366 kb on + strand, within CA265_RS20005 at 4947.397 kb on - strand, within CA265_RS20005 at 4947.448 kb on - strand, within CA265_RS20005 at 4947.448 kb on - strand, within CA265_RS20005 at 4947.449 kb on + strand, within CA265_RS20005 at 4947.465 kb on + strand, within CA265_RS20005 at 4947.514 kb on - strand, within CA265_RS20005 at 4947.524 kb on - strand, within CA265_RS20005 at 4947.524 kb on - strand, within CA265_RS20005 at 4947.537 kb on + strand, within CA265_RS20005 at 4947.583 kb on - strand, within CA265_RS20005 at 4947.625 kb on + strand at 4947.669 kb on + strand at 4947.670 kb on - strand at 4947.708 kb on + strand at 4947.708 kb on + strand at 4947.710 kb on + strand at 4947.710 kb on + strand at 4947.711 kb on - strand at 4947.711 kb on - strand at 4947.711 kb on - strand at 4947.711 kb on - strand at 4947.711 kb on - strand at 4947.720 kb on + strand, within CA265_RS20010 at 4947.721 kb on - strand, within CA265_RS20010 at 4947.748 kb on + strand, within CA265_RS20010 at 4947.799 kb on - strand, within CA265_RS20010 at 4947.830 kb on - strand, within CA265_RS20010 at 4947.895 kb on - strand, within CA265_RS20010 at 4947.976 kb on + strand, within CA265_RS20010 at 4948.039 kb on + strand, within CA265_RS20010 at 4948.040 kb on - strand, within CA265_RS20010 at 4948.069 kb on - strand at 4948.147 kb on - strand at 4948.170 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 4,946,704 + +0.7 4,946,704 + +0.1 4,946,708 + +0.2 4,946,709 - +0.6 4,946,741 + -0.7 4,946,800 - -1.0 4,946,811 - -0.2 4,946,891 + +0.3 4,946,992 - CA265_RS20005 0.13 -0.5 4,947,009 + CA265_RS20005 0.15 +0.2 4,947,009 + CA265_RS20005 0.15 +0.2 4,947,130 + CA265_RS20005 0.31 -0.1 4,947,168 + -1.2 4,947,191 - CA265_RS20005 0.39 +0.5 4,947,199 - CA265_RS20005 0.40 -0.6 4,947,261 + CA265_RS20005 0.48 +0.3 4,947,261 + CA265_RS20005 0.48 -0.9 4,947,261 + CA265_RS20005 0.48 -0.0 4,947,304 + CA265_RS20005 0.53 -0.7 4,947,305 - CA265_RS20005 0.53 -0.7 4,947,305 - CA265_RS20005 0.53 +0.6 4,947,366 + CA265_RS20005 0.61 +1.6 4,947,397 - CA265_RS20005 0.65 -0.7 4,947,448 - CA265_RS20005 0.72 +0.6 4,947,448 - CA265_RS20005 0.72 -0.0 4,947,449 + CA265_RS20005 0.72 +0.2 4,947,465 + CA265_RS20005 0.74 -0.5 4,947,514 - CA265_RS20005 0.80 +0.3 4,947,524 - CA265_RS20005 0.81 +0.1 4,947,524 - CA265_RS20005 0.81 +0.2 4,947,537 + CA265_RS20005 0.83 +0.5 4,947,583 - CA265_RS20005 0.89 +1.0 4,947,625 + +0.3 4,947,669 + +0.8 4,947,670 - -0.0 4,947,708 + +1.1 4,947,708 + -0.6 4,947,710 + +0.2 4,947,710 + +2.0 4,947,711 - -0.3 4,947,711 - +0.8 4,947,711 - -1.3 4,947,711 - -0.7 4,947,711 - +0.6 4,947,720 + CA265_RS20010 0.11 -0.0 4,947,721 - CA265_RS20010 0.11 +0.1 4,947,748 + CA265_RS20010 0.18 -0.4 4,947,799 - CA265_RS20010 0.30 +0.3 4,947,830 - CA265_RS20010 0.37 +1.4 4,947,895 - CA265_RS20010 0.53 +1.0 4,947,976 + CA265_RS20010 0.72 -0.4 4,948,039 + CA265_RS20010 0.87 -1.3 4,948,040 - CA265_RS20010 0.87 +0.5 4,948,069 - +0.9 4,948,147 - +2.9 4,948,170 + +1.6
Or see this region's nucleotide sequence