Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18520 and CA265_RS18525 are separated by 29 nucleotides CA265_RS18525 and CA265_RS18530 are separated by 4 nucleotides CA265_RS18530 and CA265_RS18535 are separated by 33 nucleotides CA265_RS18535 and CA265_RS18540 are separated by 40 nucleotides
CA265_RS18520: CA265_RS18520 - acetylornithine carbamoyltransferase, at 4,605,266 to 4,606,240
_RS18520
CA265_RS18525: CA265_RS18525 - four helix bundle protein, at 4,606,270 to 4,606,644
_RS18525
CA265_RS18530: CA265_RS18530 - aspartate aminotransferase family protein, at 4,606,649 to 4,607,797
_RS18530
CA265_RS18535: CA265_RS18535 - four helix bundle protein, at 4,607,831 to 4,608,202
_RS18535
CA265_RS18540: CA265_RS18540 - N-succinylglutamylphosphate reductase (EC 1.2.1.38) (from data) , at 4,608,243 to 4,609,223
_RS18540
Position (kb)
4606
4607
4608 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4605.727 kb on + strand, within CA265_RS18520 at 4605.728 kb on - strand, within CA265_RS18520 at 4605.775 kb on + strand, within CA265_RS18520 at 4605.776 kb on - strand, within CA265_RS18520 at 4605.843 kb on + strand, within CA265_RS18520 at 4605.875 kb on - strand, within CA265_RS18520 at 4605.934 kb on - strand, within CA265_RS18520 at 4605.934 kb on - strand, within CA265_RS18520 at 4605.965 kb on - strand, within CA265_RS18520 at 4605.989 kb on + strand, within CA265_RS18520 at 4605.990 kb on - strand, within CA265_RS18520 at 4606.054 kb on + strand, within CA265_RS18520 at 4606.055 kb on - strand, within CA265_RS18520 at 4606.055 kb on - strand, within CA265_RS18520 at 4606.065 kb on - strand, within CA265_RS18520 at 4606.085 kb on + strand, within CA265_RS18520 at 4606.097 kb on + strand, within CA265_RS18520 at 4606.191 kb on - strand at 4606.250 kb on + strand at 4606.264 kb on + strand at 4606.357 kb on + strand, within CA265_RS18525 at 4606.357 kb on + strand, within CA265_RS18525 at 4606.361 kb on + strand at 4606.399 kb on + strand, within CA265_RS18525 at 4606.399 kb on + strand, within CA265_RS18525 at 4606.409 kb on + strand, within CA265_RS18525 at 4606.440 kb on + strand, within CA265_RS18525 at 4606.506 kb on + strand, within CA265_RS18525 at 4606.510 kb on + strand, within CA265_RS18525 at 4606.522 kb on - strand, within CA265_RS18525 at 4606.597 kb on + strand, within CA265_RS18525 at 4606.865 kb on + strand, within CA265_RS18530 at 4607.044 kb on + strand, within CA265_RS18530 at 4607.092 kb on + strand, within CA265_RS18530 at 4607.143 kb on + strand, within CA265_RS18530 at 4607.162 kb on + strand, within CA265_RS18530 at 4607.178 kb on + strand, within CA265_RS18530 at 4607.191 kb on - strand, within CA265_RS18530 at 4607.586 kb on - strand, within CA265_RS18530 at 4607.627 kb on + strand, within CA265_RS18530 at 4607.809 kb on + strand at 4607.809 kb on + strand at 4607.810 kb on - strand at 4607.811 kb on + strand at 4607.877 kb on - strand, within CA265_RS18535 at 4607.910 kb on - strand, within CA265_RS18535 at 4607.957 kb on + strand, within CA265_RS18535 at 4607.972 kb on + strand, within CA265_RS18535 at 4608.096 kb on - strand, within CA265_RS18535 at 4608.151 kb on + strand, within CA265_RS18535 at 4608.152 kb on - strand, within CA265_RS18535 at 4608.156 kb on - strand, within CA265_RS18535 at 4608.207 kb on + strand at 4608.240 kb on + strand at 4608.470 kb on + strand, within CA265_RS18540 at 4608.505 kb on - strand, within CA265_RS18540 at 4608.671 kb on + strand, within CA265_RS18540 at 4608.734 kb on + strand, within CA265_RS18540
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 4,605,727 + CA265_RS18520 0.47 -0.5 4,605,728 - CA265_RS18520 0.47 +3.1 4,605,775 + CA265_RS18520 0.52 -0.1 4,605,776 - CA265_RS18520 0.52 -0.1 4,605,843 + CA265_RS18520 0.59 +1.4 4,605,875 - CA265_RS18520 0.62 -0.2 4,605,934 - CA265_RS18520 0.69 -1.6 4,605,934 - CA265_RS18520 0.69 +0.0 4,605,965 - CA265_RS18520 0.72 -0.6 4,605,989 + CA265_RS18520 0.74 +0.4 4,605,990 - CA265_RS18520 0.74 -0.3 4,606,054 + CA265_RS18520 0.81 -0.5 4,606,055 - CA265_RS18520 0.81 +0.2 4,606,055 - CA265_RS18520 0.81 -1.0 4,606,065 - CA265_RS18520 0.82 +0.3 4,606,085 + CA265_RS18520 0.84 +0.0 4,606,097 + CA265_RS18520 0.85 +0.5 4,606,191 - -0.6 4,606,250 + +0.1 4,606,264 + -0.8 4,606,357 + CA265_RS18525 0.23 +1.5 4,606,357 + CA265_RS18525 0.23 +0.3 4,606,361 + +0.3 4,606,399 + CA265_RS18525 0.34 -1.8 4,606,399 + CA265_RS18525 0.34 -1.0 4,606,409 + CA265_RS18525 0.37 -0.6 4,606,440 + CA265_RS18525 0.45 -0.3 4,606,506 + CA265_RS18525 0.63 -0.3 4,606,510 + CA265_RS18525 0.64 +0.1 4,606,522 - CA265_RS18525 0.67 -1.5 4,606,597 + CA265_RS18525 0.87 -0.0 4,606,865 + CA265_RS18530 0.19 -1.3 4,607,044 + CA265_RS18530 0.34 -0.5 4,607,092 + CA265_RS18530 0.39 -0.8 4,607,143 + CA265_RS18530 0.43 -1.2 4,607,162 + CA265_RS18530 0.45 +0.9 4,607,178 + CA265_RS18530 0.46 +0.3 4,607,191 - CA265_RS18530 0.47 -1.3 4,607,586 - CA265_RS18530 0.82 -1.5 4,607,627 + CA265_RS18530 0.85 -1.5 4,607,809 + -0.6 4,607,809 + +0.8 4,607,810 - -2.3 4,607,811 + -0.1 4,607,877 - CA265_RS18535 0.12 +0.2 4,607,910 - CA265_RS18535 0.21 +0.7 4,607,957 + CA265_RS18535 0.34 +1.0 4,607,972 + CA265_RS18535 0.38 -0.2 4,608,096 - CA265_RS18535 0.71 +0.5 4,608,151 + CA265_RS18535 0.86 -0.0 4,608,152 - CA265_RS18535 0.86 +0.3 4,608,156 - CA265_RS18535 0.87 -0.2 4,608,207 + -0.5 4,608,240 + -1.1 4,608,470 + CA265_RS18540 0.23 +1.0 4,608,505 - CA265_RS18540 0.27 -1.3 4,608,671 + CA265_RS18540 0.44 +0.9 4,608,734 + CA265_RS18540 0.50 -1.3
Or see this region's nucleotide sequence