Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18140 and CA265_RS18145 are separated by 124 nucleotides CA265_RS18145 and CA265_RS18150 are separated by 152 nucleotides
CA265_RS18140: CA265_RS18140 - DUF4271 domain-containing protein, at 4,527,827 to 4,528,804
_RS18140
CA265_RS18145: CA265_RS18145 - hypothetical protein, at 4,528,929 to 4,529,156
_RS18145
CA265_RS18150: CA265_RS18150 - hypothetical protein, at 4,529,309 to 4,529,989
_RS18150
Position (kb)
4528
4529
4530 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4527.947 kb on + strand, within CA265_RS18140 at 4527.997 kb on - strand, within CA265_RS18140 at 4528.012 kb on + strand, within CA265_RS18140 at 4528.178 kb on - strand, within CA265_RS18140 at 4528.178 kb on - strand, within CA265_RS18140 at 4528.188 kb on - strand, within CA265_RS18140 at 4528.189 kb on + strand, within CA265_RS18140 at 4528.189 kb on + strand, within CA265_RS18140 at 4528.189 kb on + strand, within CA265_RS18140 at 4528.224 kb on + strand, within CA265_RS18140 at 4528.224 kb on + strand, within CA265_RS18140 at 4528.225 kb on - strand, within CA265_RS18140 at 4528.336 kb on + strand, within CA265_RS18140 at 4528.343 kb on + strand, within CA265_RS18140 at 4528.430 kb on - strand, within CA265_RS18140 at 4528.518 kb on + strand, within CA265_RS18140 at 4528.518 kb on + strand, within CA265_RS18140 at 4528.519 kb on - strand, within CA265_RS18140 at 4528.607 kb on - strand, within CA265_RS18140 at 4528.686 kb on + strand, within CA265_RS18140 at 4528.693 kb on + strand, within CA265_RS18140 at 4528.694 kb on - strand, within CA265_RS18140 at 4528.721 kb on - strand at 4528.752 kb on - strand at 4528.810 kb on - strand at 4528.908 kb on + strand at 4528.924 kb on + strand at 4528.927 kb on + strand at 4529.046 kb on - strand, within CA265_RS18145 at 4529.081 kb on + strand, within CA265_RS18145 at 4529.122 kb on + strand, within CA265_RS18145 at 4529.122 kb on + strand, within CA265_RS18145 at 4529.130 kb on + strand, within CA265_RS18145 at 4529.131 kb on - strand, within CA265_RS18145 at 4529.176 kb on + strand at 4529.181 kb on - strand at 4529.189 kb on + strand at 4529.208 kb on + strand at 4529.209 kb on - strand at 4529.214 kb on - strand at 4529.219 kb on + strand at 4529.219 kb on + strand at 4529.220 kb on - strand at 4529.220 kb on - strand at 4529.220 kb on - strand at 4529.222 kb on - strand at 4529.253 kb on - strand at 4529.256 kb on + strand at 4529.275 kb on - strand at 4529.311 kb on - strand at 4529.399 kb on - strand, within CA265_RS18150 at 4529.441 kb on + strand, within CA265_RS18150 at 4529.446 kb on + strand, within CA265_RS18150 at 4529.446 kb on + strand, within CA265_RS18150 at 4529.462 kb on + strand, within CA265_RS18150 at 4529.462 kb on + strand, within CA265_RS18150 at 4529.473 kb on + strand, within CA265_RS18150 at 4529.474 kb on - strand, within CA265_RS18150 at 4529.479 kb on - strand, within CA265_RS18150 at 4529.482 kb on + strand, within CA265_RS18150 at 4529.482 kb on + strand, within CA265_RS18150 at 4529.537 kb on - strand, within CA265_RS18150 at 4529.552 kb on - strand, within CA265_RS18150 at 4529.552 kb on - strand, within CA265_RS18150 at 4529.555 kb on + strand, within CA265_RS18150 at 4529.556 kb on - strand, within CA265_RS18150 at 4529.588 kb on + strand, within CA265_RS18150 at 4529.589 kb on - strand, within CA265_RS18150 at 4529.589 kb on - strand, within CA265_RS18150 at 4529.589 kb on - strand, within CA265_RS18150 at 4529.589 kb on - strand, within CA265_RS18150 at 4529.590 kb on + strand, within CA265_RS18150 at 4529.590 kb on + strand, within CA265_RS18150 at 4529.590 kb on + strand, within CA265_RS18150 at 4529.591 kb on - strand, within CA265_RS18150 at 4529.591 kb on - strand, within CA265_RS18150 at 4529.591 kb on - strand, within CA265_RS18150 at 4529.592 kb on + strand, within CA265_RS18150 at 4529.592 kb on + strand, within CA265_RS18150 at 4529.702 kb on + strand, within CA265_RS18150 at 4529.703 kb on - strand, within CA265_RS18150 at 4529.721 kb on + strand, within CA265_RS18150 at 4529.752 kb on + strand, within CA265_RS18150 at 4529.775 kb on - strand, within CA265_RS18150 at 4529.813 kb on - strand, within CA265_RS18150 at 4529.815 kb on - strand, within CA265_RS18150 at 4529.827 kb on + strand, within CA265_RS18150 at 4529.885 kb on + strand, within CA265_RS18150 at 4529.927 kb on - strand at 4529.964 kb on + strand at 4530.075 kb on + strand at 4530.120 kb on + strand at 4530.126 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 4,527,947 + CA265_RS18140 0.12 -1.1 4,527,997 - CA265_RS18140 0.17 +0.5 4,528,012 + CA265_RS18140 0.19 -1.1 4,528,178 - CA265_RS18140 0.36 +0.3 4,528,178 - CA265_RS18140 0.36 -1.3 4,528,188 - CA265_RS18140 0.37 -0.1 4,528,189 + CA265_RS18140 0.37 +0.9 4,528,189 + CA265_RS18140 0.37 -0.2 4,528,189 + CA265_RS18140 0.37 +0.1 4,528,224 + CA265_RS18140 0.41 +0.8 4,528,224 + CA265_RS18140 0.41 +0.6 4,528,225 - CA265_RS18140 0.41 -0.3 4,528,336 + CA265_RS18140 0.52 -1.5 4,528,343 + CA265_RS18140 0.53 +0.3 4,528,430 - CA265_RS18140 0.62 -1.0 4,528,518 + CA265_RS18140 0.71 -0.1 4,528,518 + CA265_RS18140 0.71 -2.9 4,528,519 - CA265_RS18140 0.71 -0.3 4,528,607 - CA265_RS18140 0.80 -0.3 4,528,686 + CA265_RS18140 0.88 -0.5 4,528,693 + CA265_RS18140 0.89 -0.3 4,528,694 - CA265_RS18140 0.89 -0.1 4,528,721 - +1.5 4,528,752 - -0.6 4,528,810 - -0.2 4,528,908 + +0.7 4,528,924 + -0.1 4,528,927 + +1.4 4,529,046 - CA265_RS18145 0.51 -0.8 4,529,081 + CA265_RS18145 0.67 -0.7 4,529,122 + CA265_RS18145 0.85 -0.4 4,529,122 + CA265_RS18145 0.85 -0.3 4,529,130 + CA265_RS18145 0.88 -0.2 4,529,131 - CA265_RS18145 0.89 -0.1 4,529,176 + +0.2 4,529,181 - -0.3 4,529,189 + +0.1 4,529,208 + +0.3 4,529,209 - +0.3 4,529,214 - +0.5 4,529,219 + +0.1 4,529,219 + +0.1 4,529,220 - -0.3 4,529,220 - +0.1 4,529,220 - +0.2 4,529,222 - +2.3 4,529,253 - -0.1 4,529,256 + -0.4 4,529,275 - +0.6 4,529,311 - -1.2 4,529,399 - CA265_RS18150 0.13 +0.3 4,529,441 + CA265_RS18150 0.19 +0.6 4,529,446 + CA265_RS18150 0.20 -0.0 4,529,446 + CA265_RS18150 0.20 -0.1 4,529,462 + CA265_RS18150 0.22 -0.5 4,529,462 + CA265_RS18150 0.22 -0.4 4,529,473 + CA265_RS18150 0.24 +0.1 4,529,474 - CA265_RS18150 0.24 +0.5 4,529,479 - CA265_RS18150 0.25 +0.4 4,529,482 + CA265_RS18150 0.25 +0.2 4,529,482 + CA265_RS18150 0.25 +0.1 4,529,537 - CA265_RS18150 0.33 -0.5 4,529,552 - CA265_RS18150 0.36 -0.8 4,529,552 - CA265_RS18150 0.36 +0.2 4,529,555 + CA265_RS18150 0.36 +0.3 4,529,556 - CA265_RS18150 0.36 -1.4 4,529,588 + CA265_RS18150 0.41 +0.1 4,529,589 - CA265_RS18150 0.41 -0.7 4,529,589 - CA265_RS18150 0.41 +0.2 4,529,589 - CA265_RS18150 0.41 -0.4 4,529,589 - CA265_RS18150 0.41 +0.8 4,529,590 + CA265_RS18150 0.41 -0.6 4,529,590 + CA265_RS18150 0.41 +0.6 4,529,590 + CA265_RS18150 0.41 -0.0 4,529,591 - CA265_RS18150 0.41 +0.5 4,529,591 - CA265_RS18150 0.41 +0.2 4,529,591 - CA265_RS18150 0.41 -0.4 4,529,592 + CA265_RS18150 0.42 +0.8 4,529,592 + CA265_RS18150 0.42 -0.6 4,529,702 + CA265_RS18150 0.58 -0.3 4,529,703 - CA265_RS18150 0.58 +0.1 4,529,721 + CA265_RS18150 0.60 +0.3 4,529,752 + CA265_RS18150 0.65 +1.1 4,529,775 - CA265_RS18150 0.68 -0.1 4,529,813 - CA265_RS18150 0.74 +0.5 4,529,815 - CA265_RS18150 0.74 +0.4 4,529,827 + CA265_RS18150 0.76 -1.5 4,529,885 + CA265_RS18150 0.85 +0.1 4,529,927 - -0.4 4,529,964 + +0.1 4,530,075 + -0.4 4,530,120 + -0.2 4,530,126 + +0.7
Or see this region's nucleotide sequence