Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14425 and CA265_RS14430 are separated by 242 nucleotides CA265_RS14430 and CA265_RS14435 are separated by 190 nucleotides CA265_RS14435 and CA265_RS14440 are separated by 125 nucleotides
CA265_RS14425: CA265_RS14425 - 1-aminocyclopropane-1-carboxylate deaminase, at 3,637,925 to 3,638,794
_RS14425
CA265_RS14430: CA265_RS14430 - carbohydrate-binding protein, at 3,639,037 to 3,640,452
_RS14430
CA265_RS14435: CA265_RS14435 - GNAT family N-acetyltransferase, at 3,640,643 to 3,640,948
_RS14435
CA265_RS14440: CA265_RS14440 - transcriptional regulator, at 3,641,074 to 3,641,439
_RS14440
Position (kb)
3639
3640
3641 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3638.048 kb on + strand, within CA265_RS14425 at 3638.124 kb on + strand, within CA265_RS14425 at 3638.146 kb on + strand, within CA265_RS14425 at 3638.203 kb on - strand, within CA265_RS14425 at 3638.203 kb on - strand, within CA265_RS14425 at 3638.318 kb on - strand, within CA265_RS14425 at 3638.391 kb on - strand, within CA265_RS14425 at 3638.392 kb on - strand, within CA265_RS14425 at 3638.439 kb on + strand, within CA265_RS14425 at 3638.483 kb on - strand, within CA265_RS14425 at 3638.487 kb on + strand, within CA265_RS14425 at 3638.494 kb on - strand, within CA265_RS14425 at 3638.526 kb on + strand, within CA265_RS14425 at 3638.526 kb on + strand, within CA265_RS14425 at 3638.530 kb on + strand, within CA265_RS14425 at 3638.531 kb on - strand, within CA265_RS14425 at 3638.534 kb on + strand, within CA265_RS14425 at 3638.632 kb on + strand, within CA265_RS14425 at 3638.632 kb on + strand, within CA265_RS14425 at 3638.633 kb on - strand, within CA265_RS14425 at 3638.633 kb on - strand, within CA265_RS14425 at 3638.717 kb on - strand at 3638.769 kb on + strand at 3638.793 kb on - strand at 3638.810 kb on - strand at 3638.810 kb on - strand at 3638.871 kb on + strand at 3638.871 kb on + strand at 3638.958 kb on + strand at 3638.963 kb on + strand at 3638.965 kb on + strand at 3638.969 kb on - strand at 3638.984 kb on - strand at 3638.984 kb on - strand at 3638.987 kb on + strand at 3638.991 kb on + strand at 3638.992 kb on - strand at 3639.099 kb on + strand at 3639.099 kb on + strand at 3639.099 kb on + strand at 3639.215 kb on - strand, within CA265_RS14430 at 3639.218 kb on + strand, within CA265_RS14430 at 3639.218 kb on + strand, within CA265_RS14430 at 3639.219 kb on - strand, within CA265_RS14430 at 3639.258 kb on + strand, within CA265_RS14430 at 3639.293 kb on + strand, within CA265_RS14430 at 3639.315 kb on + strand, within CA265_RS14430 at 3639.342 kb on + strand, within CA265_RS14430 at 3639.366 kb on + strand, within CA265_RS14430 at 3639.367 kb on - strand, within CA265_RS14430 at 3639.425 kb on - strand, within CA265_RS14430 at 3639.425 kb on - strand, within CA265_RS14430 at 3639.478 kb on + strand, within CA265_RS14430 at 3639.513 kb on + strand, within CA265_RS14430 at 3639.523 kb on + strand, within CA265_RS14430 at 3639.523 kb on + strand, within CA265_RS14430 at 3639.524 kb on - strand, within CA265_RS14430 at 3639.577 kb on + strand, within CA265_RS14430 at 3639.661 kb on - strand, within CA265_RS14430 at 3639.671 kb on - strand, within CA265_RS14430 at 3639.755 kb on + strand, within CA265_RS14430 at 3639.772 kb on - strand, within CA265_RS14430 at 3639.831 kb on + strand, within CA265_RS14430 at 3639.832 kb on - strand, within CA265_RS14430 at 3639.832 kb on - strand, within CA265_RS14430 at 3639.837 kb on + strand, within CA265_RS14430 at 3639.838 kb on - strand, within CA265_RS14430 at 3639.838 kb on - strand, within CA265_RS14430 at 3639.951 kb on + strand, within CA265_RS14430 at 3640.022 kb on + strand, within CA265_RS14430 at 3640.102 kb on + strand, within CA265_RS14430 at 3640.102 kb on + strand, within CA265_RS14430 at 3640.103 kb on - strand, within CA265_RS14430 at 3640.227 kb on + strand, within CA265_RS14430 at 3640.294 kb on - strand, within CA265_RS14430 at 3640.295 kb on + strand, within CA265_RS14430 at 3640.345 kb on - strand at 3640.369 kb on - strand at 3640.442 kb on - strand at 3640.462 kb on + strand at 3640.463 kb on - strand at 3640.465 kb on + strand at 3640.502 kb on + strand at 3640.529 kb on - strand at 3640.529 kb on - strand at 3640.530 kb on + strand at 3640.531 kb on - strand at 3640.609 kb on - strand at 3640.609 kb on - strand at 3640.712 kb on + strand, within CA265_RS14435 at 3640.712 kb on + strand, within CA265_RS14435 at 3640.713 kb on - strand, within CA265_RS14435 at 3640.713 kb on - strand, within CA265_RS14435 at 3640.717 kb on - strand, within CA265_RS14435 at 3640.745 kb on - strand, within CA265_RS14435 at 3640.782 kb on - strand, within CA265_RS14435 at 3640.928 kb on - strand at 3640.932 kb on - strand at 3640.954 kb on - strand at 3640.963 kb on - strand at 3641.031 kb on + strand at 3641.043 kb on + strand at 3641.061 kb on + strand at 3641.100 kb on + strand at 3641.122 kb on + strand, within CA265_RS14440 at 3641.177 kb on - strand, within CA265_RS14440 at 3641.230 kb on + strand, within CA265_RS14440 at 3641.267 kb on - strand, within CA265_RS14440 at 3641.268 kb on + strand, within CA265_RS14440 at 3641.269 kb on - strand, within CA265_RS14440 at 3641.333 kb on - strand, within CA265_RS14440 at 3641.441 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 3,638,048 + CA265_RS14425 0.14 +1.0 3,638,124 + CA265_RS14425 0.23 +0.4 3,638,146 + CA265_RS14425 0.25 -0.2 3,638,203 - CA265_RS14425 0.32 -0.7 3,638,203 - CA265_RS14425 0.32 +0.2 3,638,318 - CA265_RS14425 0.45 -0.6 3,638,391 - CA265_RS14425 0.54 +0.6 3,638,392 - CA265_RS14425 0.54 -0.1 3,638,439 + CA265_RS14425 0.59 -1.3 3,638,483 - CA265_RS14425 0.64 +0.0 3,638,487 + CA265_RS14425 0.65 +0.1 3,638,494 - CA265_RS14425 0.65 +0.8 3,638,526 + CA265_RS14425 0.69 +0.9 3,638,526 + CA265_RS14425 0.69 +0.2 3,638,530 + CA265_RS14425 0.70 -0.5 3,638,531 - CA265_RS14425 0.70 +0.1 3,638,534 + CA265_RS14425 0.70 -0.5 3,638,632 + CA265_RS14425 0.81 -0.6 3,638,632 + CA265_RS14425 0.81 -0.3 3,638,633 - CA265_RS14425 0.81 -0.4 3,638,633 - CA265_RS14425 0.81 -0.1 3,638,717 - -0.5 3,638,769 + +0.6 3,638,793 - +0.2 3,638,810 - -0.1 3,638,810 - +0.5 3,638,871 + -0.8 3,638,871 + +0.3 3,638,958 + +0.3 3,638,963 + +1.5 3,638,965 + -0.9 3,638,969 - -0.6 3,638,984 - -0.8 3,638,984 - -0.5 3,638,987 + +0.5 3,638,991 + -1.9 3,638,992 - -0.7 3,639,099 + -0.1 3,639,099 + +0.3 3,639,099 + +0.2 3,639,215 - CA265_RS14430 0.13 -0.4 3,639,218 + CA265_RS14430 0.13 +0.1 3,639,218 + CA265_RS14430 0.13 +0.2 3,639,219 - CA265_RS14430 0.13 +0.4 3,639,258 + CA265_RS14430 0.16 +0.8 3,639,293 + CA265_RS14430 0.18 -0.5 3,639,315 + CA265_RS14430 0.20 -0.1 3,639,342 + CA265_RS14430 0.22 -0.1 3,639,366 + CA265_RS14430 0.23 -0.3 3,639,367 - CA265_RS14430 0.23 +0.9 3,639,425 - CA265_RS14430 0.27 +0.3 3,639,425 - CA265_RS14430 0.27 -0.8 3,639,478 + CA265_RS14430 0.31 +0.3 3,639,513 + CA265_RS14430 0.34 -0.3 3,639,523 + CA265_RS14430 0.34 +0.2 3,639,523 + CA265_RS14430 0.34 -0.1 3,639,524 - CA265_RS14430 0.34 +0.5 3,639,577 + CA265_RS14430 0.38 +0.6 3,639,661 - CA265_RS14430 0.44 -0.4 3,639,671 - CA265_RS14430 0.45 +1.3 3,639,755 + CA265_RS14430 0.51 -0.6 3,639,772 - CA265_RS14430 0.52 +0.2 3,639,831 + CA265_RS14430 0.56 +0.6 3,639,832 - CA265_RS14430 0.56 +0.6 3,639,832 - CA265_RS14430 0.56 -0.4 3,639,837 + CA265_RS14430 0.56 -1.6 3,639,838 - CA265_RS14430 0.57 -0.3 3,639,838 - CA265_RS14430 0.57 +0.1 3,639,951 + CA265_RS14430 0.65 -0.1 3,640,022 + CA265_RS14430 0.70 -0.2 3,640,102 + CA265_RS14430 0.75 -1.5 3,640,102 + CA265_RS14430 0.75 -0.7 3,640,103 - CA265_RS14430 0.75 +0.3 3,640,227 + CA265_RS14430 0.84 +0.3 3,640,294 - CA265_RS14430 0.89 +0.0 3,640,295 + CA265_RS14430 0.89 -0.1 3,640,345 - -1.7 3,640,369 - -0.6 3,640,442 - -1.1 3,640,462 + -0.3 3,640,463 - +0.9 3,640,465 + +0.5 3,640,502 + -0.5 3,640,529 - +0.4 3,640,529 - +0.9 3,640,530 + -0.4 3,640,531 - +0.2 3,640,609 - -0.5 3,640,609 - -0.7 3,640,712 + CA265_RS14435 0.23 -0.1 3,640,712 + CA265_RS14435 0.23 +0.4 3,640,713 - CA265_RS14435 0.23 -0.2 3,640,713 - CA265_RS14435 0.23 +0.7 3,640,717 - CA265_RS14435 0.24 -0.0 3,640,745 - CA265_RS14435 0.33 +0.6 3,640,782 - CA265_RS14435 0.45 +0.3 3,640,928 - +0.1 3,640,932 - -3.0 3,640,954 - +0.1 3,640,963 - +0.2 3,641,031 + +0.1 3,641,043 + +0.1 3,641,061 + +0.2 3,641,100 + +0.2 3,641,122 + CA265_RS14440 0.13 -1.1 3,641,177 - CA265_RS14440 0.28 -1.3 3,641,230 + CA265_RS14440 0.43 +0.8 3,641,267 - CA265_RS14440 0.53 +0.1 3,641,268 + CA265_RS14440 0.53 -0.0 3,641,269 - CA265_RS14440 0.53 +0.2 3,641,333 - CA265_RS14440 0.71 -0.8 3,641,441 - +0.3
Or see this region's nucleotide sequence