Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08135 and CA265_RS08140 are separated by 43 nucleotides CA265_RS08140 and CA265_RS08145 are separated by 280 nucleotides
CA265_RS08135: CA265_RS08135 - amidohydrolase, at 2,018,112 to 2,018,891
_RS08135
CA265_RS08140: CA265_RS08140 - methionine aminotransferase, at 2,018,935 to 2,020,080
_RS08140
CA265_RS08145: CA265_RS08145 - hypothetical protein, at 2,020,361 to 2,021,893
_RS08145
Position (kb)
2018
2019
2020
2021 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2017.955 kb on + strand at 2017.996 kb on + strand at 2018.014 kb on + strand at 2018.030 kb on + strand at 2018.054 kb on + strand at 2018.055 kb on - strand at 2018.163 kb on + strand at 2018.224 kb on + strand, within CA265_RS08135 at 2018.227 kb on + strand, within CA265_RS08135 at 2018.227 kb on + strand, within CA265_RS08135 at 2018.321 kb on - strand, within CA265_RS08135 at 2018.321 kb on - strand, within CA265_RS08135 at 2018.451 kb on + strand, within CA265_RS08135 at 2018.502 kb on - strand, within CA265_RS08135 at 2018.721 kb on + strand, within CA265_RS08135 at 2018.775 kb on - strand, within CA265_RS08135 at 2018.775 kb on - strand, within CA265_RS08135 at 2018.810 kb on + strand, within CA265_RS08135 at 2018.810 kb on + strand, within CA265_RS08135 at 2018.810 kb on + strand, within CA265_RS08135 at 2018.811 kb on - strand, within CA265_RS08135 at 2018.874 kb on + strand at 2018.899 kb on + strand at 2018.900 kb on - strand at 2019.108 kb on + strand, within CA265_RS08140 at 2019.119 kb on + strand, within CA265_RS08140 at 2019.119 kb on + strand, within CA265_RS08140 at 2019.120 kb on - strand, within CA265_RS08140 at 2019.120 kb on - strand, within CA265_RS08140 at 2019.125 kb on - strand, within CA265_RS08140 at 2019.265 kb on - strand, within CA265_RS08140 at 2019.283 kb on - strand, within CA265_RS08140 at 2019.372 kb on + strand, within CA265_RS08140 at 2019.400 kb on + strand, within CA265_RS08140 at 2019.401 kb on - strand, within CA265_RS08140 at 2019.403 kb on - strand, within CA265_RS08140 at 2019.403 kb on - strand, within CA265_RS08140 at 2019.439 kb on + strand, within CA265_RS08140 at 2019.440 kb on - strand, within CA265_RS08140 at 2019.510 kb on + strand, within CA265_RS08140 at 2019.525 kb on + strand, within CA265_RS08140 at 2019.586 kb on + strand, within CA265_RS08140 at 2019.591 kb on + strand, within CA265_RS08140 at 2019.592 kb on - strand, within CA265_RS08140 at 2019.630 kb on + strand, within CA265_RS08140 at 2019.642 kb on + strand at 2019.643 kb on - strand, within CA265_RS08140 at 2019.648 kb on - strand, within CA265_RS08140 at 2019.648 kb on - strand, within CA265_RS08140 at 2019.648 kb on - strand, within CA265_RS08140 at 2019.720 kb on - strand, within CA265_RS08140 at 2019.738 kb on + strand, within CA265_RS08140 at 2019.758 kb on - strand, within CA265_RS08140 at 2019.759 kb on + strand, within CA265_RS08140 at 2019.760 kb on - strand, within CA265_RS08140 at 2019.843 kb on - strand, within CA265_RS08140 at 2019.933 kb on + strand, within CA265_RS08140 at 2019.994 kb on - strand at 2020.045 kb on - strand at 2020.049 kb on - strand at 2020.069 kb on + strand at 2020.069 kb on + strand at 2020.075 kb on - strand at 2020.083 kb on + strand at 2020.086 kb on - strand at 2020.110 kb on + strand at 2020.130 kb on + strand at 2020.131 kb on - strand at 2020.131 kb on - strand at 2020.232 kb on + strand at 2020.277 kb on - strand at 2020.288 kb on - strand at 2020.297 kb on + strand at 2020.356 kb on + strand at 2020.361 kb on - strand at 2020.401 kb on + strand at 2020.482 kb on + strand at 2020.482 kb on + strand at 2020.483 kb on - strand at 2020.483 kb on - strand at 2020.542 kb on + strand, within CA265_RS08145 at 2020.543 kb on - strand, within CA265_RS08145 at 2020.552 kb on - strand, within CA265_RS08145 at 2020.662 kb on + strand, within CA265_RS08145 at 2020.680 kb on - strand, within CA265_RS08145 at 2020.717 kb on + strand, within CA265_RS08145 at 2020.723 kb on + strand, within CA265_RS08145 at 2020.817 kb on + strand, within CA265_RS08145 at 2020.818 kb on - strand, within CA265_RS08145 at 2020.838 kb on - strand, within CA265_RS08145 at 2020.869 kb on + strand, within CA265_RS08145 at 2020.875 kb on + strand, within CA265_RS08145 at 2020.897 kb on + strand, within CA265_RS08145 at 2020.897 kb on + strand, within CA265_RS08145 at 2020.898 kb on - strand, within CA265_RS08145 at 2020.906 kb on + strand, within CA265_RS08145 at 2020.907 kb on - strand, within CA265_RS08145 at 2020.907 kb on - strand, within CA265_RS08145 at 2020.950 kb on + strand, within CA265_RS08145 at 2020.950 kb on + strand, within CA265_RS08145 at 2020.950 kb on + strand, within CA265_RS08145 at 2020.961 kb on - strand, within CA265_RS08145 at 2020.978 kb on - strand, within CA265_RS08145 at 2020.982 kb on - strand, within CA265_RS08145 at 2021.003 kb on - strand, within CA265_RS08145 at 2021.005 kb on - strand, within CA265_RS08145
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 2,017,955 + +0.2 2,017,996 + +0.6 2,018,014 + -0.8 2,018,030 + -0.3 2,018,054 + +0.6 2,018,055 - -0.1 2,018,163 + +0.3 2,018,224 + CA265_RS08135 0.14 +0.5 2,018,227 + CA265_RS08135 0.15 -0.7 2,018,227 + CA265_RS08135 0.15 +0.5 2,018,321 - CA265_RS08135 0.27 +1.3 2,018,321 - CA265_RS08135 0.27 -1.1 2,018,451 + CA265_RS08135 0.43 -0.5 2,018,502 - CA265_RS08135 0.50 -1.3 2,018,721 + CA265_RS08135 0.78 -0.8 2,018,775 - CA265_RS08135 0.85 +0.6 2,018,775 - CA265_RS08135 0.85 -1.1 2,018,810 + CA265_RS08135 0.89 +0.9 2,018,810 + CA265_RS08135 0.89 -0.1 2,018,810 + CA265_RS08135 0.89 +0.1 2,018,811 - CA265_RS08135 0.90 -0.4 2,018,874 + -0.2 2,018,899 + -0.4 2,018,900 - -0.5 2,019,108 + CA265_RS08140 0.15 +0.6 2,019,119 + CA265_RS08140 0.16 +0.6 2,019,119 + CA265_RS08140 0.16 +0.8 2,019,120 - CA265_RS08140 0.16 -0.8 2,019,120 - CA265_RS08140 0.16 +0.6 2,019,125 - CA265_RS08140 0.17 -0.9 2,019,265 - CA265_RS08140 0.29 +0.2 2,019,283 - CA265_RS08140 0.30 +0.5 2,019,372 + CA265_RS08140 0.38 -0.1 2,019,400 + CA265_RS08140 0.41 +1.2 2,019,401 - CA265_RS08140 0.41 +1.1 2,019,403 - CA265_RS08140 0.41 +0.2 2,019,403 - CA265_RS08140 0.41 +0.2 2,019,439 + CA265_RS08140 0.44 +0.0 2,019,440 - CA265_RS08140 0.44 +0.2 2,019,510 + CA265_RS08140 0.50 -0.1 2,019,525 + CA265_RS08140 0.51 +0.4 2,019,586 + CA265_RS08140 0.57 +1.0 2,019,591 + CA265_RS08140 0.57 -0.5 2,019,592 - CA265_RS08140 0.57 -0.5 2,019,630 + CA265_RS08140 0.61 -0.0 2,019,642 + +0.1 2,019,643 - CA265_RS08140 0.62 +0.2 2,019,648 - CA265_RS08140 0.62 -0.7 2,019,648 - CA265_RS08140 0.62 -0.3 2,019,648 - CA265_RS08140 0.62 +1.2 2,019,720 - CA265_RS08140 0.68 -0.8 2,019,738 + CA265_RS08140 0.70 -0.4 2,019,758 - CA265_RS08140 0.72 -0.1 2,019,759 + CA265_RS08140 0.72 -0.7 2,019,760 - CA265_RS08140 0.72 +0.5 2,019,843 - CA265_RS08140 0.79 -0.2 2,019,933 + CA265_RS08140 0.87 +0.1 2,019,994 - +0.4 2,020,045 - +0.2 2,020,049 - +1.1 2,020,069 + +0.1 2,020,069 + +0.4 2,020,075 - +0.0 2,020,083 + +0.4 2,020,086 - -3.0 2,020,110 + -0.4 2,020,130 + +1.3 2,020,131 - +0.6 2,020,131 - -1.5 2,020,232 + +0.9 2,020,277 - +0.3 2,020,288 - -0.3 2,020,297 + -1.3 2,020,356 + -0.9 2,020,361 - -0.2 2,020,401 + -0.3 2,020,482 + +0.1 2,020,482 + +0.8 2,020,483 - -1.4 2,020,483 - -0.2 2,020,542 + CA265_RS08145 0.12 -0.2 2,020,543 - CA265_RS08145 0.12 +0.7 2,020,552 - CA265_RS08145 0.12 -0.0 2,020,662 + CA265_RS08145 0.20 +0.4 2,020,680 - CA265_RS08145 0.21 -0.7 2,020,717 + CA265_RS08145 0.23 -0.5 2,020,723 + CA265_RS08145 0.24 +0.2 2,020,817 + CA265_RS08145 0.30 +0.1 2,020,818 - CA265_RS08145 0.30 +1.1 2,020,838 - CA265_RS08145 0.31 +0.2 2,020,869 + CA265_RS08145 0.33 +0.8 2,020,875 + CA265_RS08145 0.34 +0.2 2,020,897 + CA265_RS08145 0.35 -0.5 2,020,897 + CA265_RS08145 0.35 -0.3 2,020,898 - CA265_RS08145 0.35 -0.3 2,020,906 + CA265_RS08145 0.36 -0.2 2,020,907 - CA265_RS08145 0.36 -0.3 2,020,907 - CA265_RS08145 0.36 +0.1 2,020,950 + CA265_RS08145 0.38 +0.1 2,020,950 + CA265_RS08145 0.38 -0.7 2,020,950 + CA265_RS08145 0.38 -0.2 2,020,961 - CA265_RS08145 0.39 +0.3 2,020,978 - CA265_RS08145 0.40 -0.2 2,020,982 - CA265_RS08145 0.41 -0.4 2,021,003 - CA265_RS08145 0.42 +0.7 2,021,005 - CA265_RS08145 0.42 +0.1
Or see this region's nucleotide sequence