Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08055 and CA265_RS08060 are separated by 350 nucleotides CA265_RS08060 and CA265_RS08065 are separated by 68 nucleotides CA265_RS08065 and CA265_RS08070 are separated by 175 nucleotides
CA265_RS08055: CA265_RS08055 - hypothetical protein, at 2,005,446 to 2,006,282
_RS08055
CA265_RS08060: CA265_RS08060 - hypothetical protein, at 2,006,633 to 2,006,947
_RS08060
CA265_RS08065: CA265_RS08065 - hypothetical protein, at 2,007,016 to 2,007,663
_RS08065
CA265_RS08070: CA265_RS08070 - tRNA-Ser, at 2,007,839 to 2,007,926
_RS08070
Position (kb)
2006
2007 Strain fitness (log2 ratio)
-2
-1
0
1 at 2005.650 kb on + strand, within CA265_RS08055 at 2005.650 kb on + strand, within CA265_RS08055 at 2005.742 kb on + strand, within CA265_RS08055 at 2005.742 kb on + strand, within CA265_RS08055 at 2005.747 kb on - strand, within CA265_RS08055 at 2005.748 kb on + strand, within CA265_RS08055 at 2005.748 kb on + strand, within CA265_RS08055 at 2005.760 kb on + strand, within CA265_RS08055 at 2005.879 kb on + strand, within CA265_RS08055 at 2005.979 kb on - strand, within CA265_RS08055 at 2006.007 kb on - strand, within CA265_RS08055 at 2006.007 kb on - strand, within CA265_RS08055 at 2006.075 kb on + strand, within CA265_RS08055 at 2006.140 kb on - strand, within CA265_RS08055 at 2006.141 kb on + strand, within CA265_RS08055 at 2006.294 kb on - strand at 2006.318 kb on + strand at 2006.351 kb on - strand at 2006.351 kb on - strand at 2006.354 kb on - strand at 2006.443 kb on + strand at 2006.444 kb on - strand at 2006.444 kb on - strand at 2006.450 kb on - strand at 2006.451 kb on + strand at 2006.455 kb on + strand at 2006.467 kb on - strand at 2006.512 kb on - strand at 2006.512 kb on - strand at 2006.630 kb on - strand at 2006.630 kb on - strand at 2006.633 kb on - strand at 2006.633 kb on - strand at 2006.642 kb on + strand at 2006.763 kb on + strand, within CA265_RS08060 at 2006.840 kb on - strand, within CA265_RS08060 at 2006.897 kb on + strand, within CA265_RS08060 at 2006.952 kb on + strand at 2006.990 kb on + strand at 2006.999 kb on - strand at 2006.999 kb on - strand at 2007.005 kb on + strand at 2007.006 kb on - strand at 2007.033 kb on + strand at 2007.034 kb on - strand at 2007.062 kb on + strand at 2007.063 kb on - strand at 2007.072 kb on + strand at 2007.073 kb on - strand at 2007.102 kb on - strand, within CA265_RS08065 at 2007.102 kb on - strand, within CA265_RS08065 at 2007.130 kb on - strand, within CA265_RS08065 at 2007.230 kb on - strand, within CA265_RS08065 at 2007.239 kb on - strand, within CA265_RS08065 at 2007.239 kb on - strand, within CA265_RS08065 at 2007.242 kb on - strand, within CA265_RS08065 at 2007.253 kb on - strand, within CA265_RS08065 at 2007.307 kb on + strand, within CA265_RS08065 at 2007.307 kb on + strand, within CA265_RS08065 at 2007.457 kb on - strand, within CA265_RS08065 at 2007.457 kb on - strand, within CA265_RS08065 at 2007.459 kb on + strand, within CA265_RS08065 at 2007.484 kb on - strand, within CA265_RS08065 at 2007.509 kb on - strand, within CA265_RS08065 at 2007.539 kb on - strand, within CA265_RS08065 at 2007.615 kb on + strand at 2007.616 kb on - strand at 2007.616 kb on - strand at 2007.651 kb on - strand at 2007.683 kb on + strand at 2007.727 kb on + strand at 2007.728 kb on - strand at 2007.876 kb on - strand, within CA265_RS08070
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 2,005,650 + CA265_RS08055 0.24 -0.0 2,005,650 + CA265_RS08055 0.24 +0.3 2,005,742 + CA265_RS08055 0.35 +0.3 2,005,742 + CA265_RS08055 0.35 -0.4 2,005,747 - CA265_RS08055 0.36 +1.1 2,005,748 + CA265_RS08055 0.36 -0.7 2,005,748 + CA265_RS08055 0.36 +1.1 2,005,760 + CA265_RS08055 0.38 +0.5 2,005,879 + CA265_RS08055 0.52 +0.3 2,005,979 - CA265_RS08055 0.64 +0.0 2,006,007 - CA265_RS08055 0.67 -0.6 2,006,007 - CA265_RS08055 0.67 -0.1 2,006,075 + CA265_RS08055 0.75 -0.4 2,006,140 - CA265_RS08055 0.83 +0.3 2,006,141 + CA265_RS08055 0.83 +0.7 2,006,294 - +0.5 2,006,318 + -0.3 2,006,351 - +0.3 2,006,351 - -0.3 2,006,354 - +0.3 2,006,443 + -0.5 2,006,444 - +0.4 2,006,444 - -0.5 2,006,450 - +0.6 2,006,451 + -0.4 2,006,455 + +0.3 2,006,467 - -0.3 2,006,512 - -0.8 2,006,512 - -2.5 2,006,630 - +0.7 2,006,630 - +0.0 2,006,633 - -0.2 2,006,633 - -0.8 2,006,642 + -0.7 2,006,763 + CA265_RS08060 0.41 -0.5 2,006,840 - CA265_RS08060 0.66 -0.6 2,006,897 + CA265_RS08060 0.84 -0.5 2,006,952 + -0.2 2,006,990 + -0.2 2,006,999 - -0.0 2,006,999 - +0.3 2,007,005 + -0.6 2,007,006 - -0.3 2,007,033 + -0.3 2,007,034 - +0.5 2,007,062 + +0.0 2,007,063 - -0.4 2,007,072 + +0.1 2,007,073 - +0.8 2,007,102 - CA265_RS08065 0.13 -0.8 2,007,102 - CA265_RS08065 0.13 -0.8 2,007,130 - CA265_RS08065 0.18 -0.5 2,007,230 - CA265_RS08065 0.33 -0.1 2,007,239 - CA265_RS08065 0.34 -0.2 2,007,239 - CA265_RS08065 0.34 +1.0 2,007,242 - CA265_RS08065 0.35 -1.0 2,007,253 - CA265_RS08065 0.37 +0.1 2,007,307 + CA265_RS08065 0.45 +0.1 2,007,307 + CA265_RS08065 0.45 +0.3 2,007,457 - CA265_RS08065 0.68 +0.9 2,007,457 - CA265_RS08065 0.68 +0.5 2,007,459 + CA265_RS08065 0.68 +0.4 2,007,484 - CA265_RS08065 0.72 +0.9 2,007,509 - CA265_RS08065 0.76 -0.9 2,007,539 - CA265_RS08065 0.81 -0.9 2,007,615 + -1.5 2,007,616 - -0.4 2,007,616 - +0.0 2,007,651 - -0.3 2,007,683 + -0.3 2,007,727 + -1.5 2,007,728 - -0.9 2,007,876 - CA265_RS08070 0.42 -0.5
Or see this region's nucleotide sequence