Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06490 and CA265_RS06495 are separated by 270 nucleotides CA265_RS06495 and CA265_RS06500 are separated by 240 nucleotides CA265_RS06500 and CA265_RS06505 are separated by 330 nucleotides CA265_RS06505 and CA265_RS06510 are separated by 228 nucleotides
CA265_RS06490: CA265_RS06490 - hypothetical protein, at 1,606,092 to 1,606,484
_RS06490
CA265_RS06495: CA265_RS06495 - hypothetical protein, at 1,606,755 to 1,607,156
_RS06495
CA265_RS06500: CA265_RS06500 - hypothetical protein, at 1,607,397 to 1,607,819
_RS06500
CA265_RS06505: CA265_RS06505 - hypothetical protein, at 1,608,150 to 1,608,500
_RS06505
CA265_RS06510: CA265_RS06510 - hypothetical protein, at 1,608,729 to 1,609,178
_RS06510
Position (kb)
1607
1608 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1606.421 kb on + strand, within CA265_RS06490 at 1606.468 kb on + strand at 1606.551 kb on - strand at 1606.614 kb on + strand at 1606.614 kb on + strand at 1606.615 kb on - strand at 1606.615 kb on - strand at 1606.615 kb on - strand at 1606.658 kb on + strand at 1606.698 kb on + strand at 1606.698 kb on + strand at 1606.722 kb on - strand at 1606.783 kb on + strand at 1606.801 kb on + strand, within CA265_RS06495 at 1606.843 kb on + strand, within CA265_RS06495 at 1606.868 kb on + strand, within CA265_RS06495 at 1606.899 kb on - strand, within CA265_RS06495 at 1606.900 kb on + strand, within CA265_RS06495 at 1606.921 kb on + strand, within CA265_RS06495 at 1606.949 kb on + strand, within CA265_RS06495 at 1606.995 kb on + strand, within CA265_RS06495 at 1606.996 kb on - strand, within CA265_RS06495 at 1606.996 kb on - strand, within CA265_RS06495 at 1606.996 kb on - strand, within CA265_RS06495 at 1606.998 kb on - strand, within CA265_RS06495 at 1606.998 kb on - strand, within CA265_RS06495 at 1606.998 kb on - strand, within CA265_RS06495 at 1607.038 kb on + strand, within CA265_RS06495 at 1607.039 kb on - strand, within CA265_RS06495 at 1607.120 kb on + strand at 1607.162 kb on + strand at 1607.246 kb on + strand at 1607.247 kb on - strand at 1607.255 kb on + strand at 1607.256 kb on - strand at 1607.336 kb on + strand at 1607.340 kb on + strand at 1607.340 kb on + strand at 1607.340 kb on + strand at 1607.551 kb on + strand, within CA265_RS06500 at 1607.551 kb on + strand, within CA265_RS06500 at 1607.552 kb on - strand, within CA265_RS06500 at 1607.558 kb on + strand, within CA265_RS06500 at 1607.559 kb on - strand, within CA265_RS06500 at 1607.578 kb on + strand, within CA265_RS06500 at 1607.625 kb on - strand, within CA265_RS06500 at 1607.625 kb on - strand, within CA265_RS06500 at 1607.631 kb on - strand, within CA265_RS06500 at 1607.721 kb on - strand, within CA265_RS06500 at 1607.776 kb on + strand, within CA265_RS06500 at 1607.784 kb on - strand at 1607.813 kb on - strand at 1607.813 kb on - strand at 1607.879 kb on + strand at 1607.909 kb on + strand at 1607.912 kb on - strand at 1608.002 kb on + strand at 1608.006 kb on + strand at 1608.008 kb on + strand at 1608.009 kb on - strand at 1608.160 kb on + strand at 1608.191 kb on - strand, within CA265_RS06505 at 1608.282 kb on - strand, within CA265_RS06505 at 1608.287 kb on + strand, within CA265_RS06505 at 1608.333 kb on + strand, within CA265_RS06505 at 1608.467 kb on + strand at 1608.467 kb on + strand at 1608.503 kb on + strand at 1608.512 kb on - strand at 1608.536 kb on + strand at 1608.537 kb on - strand at 1608.538 kb on + strand at 1608.539 kb on - strand at 1608.573 kb on - strand at 1608.600 kb on - strand at 1608.600 kb on - strand at 1608.640 kb on + strand at 1608.680 kb on + strand at 1608.681 kb on - strand at 1608.691 kb on + strand at 1608.691 kb on + strand at 1608.692 kb on - strand at 1608.720 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 1,606,421 + CA265_RS06490 0.84 -0.4 1,606,468 + +0.4 1,606,551 - +0.3 1,606,614 + +0.2 1,606,614 + -0.8 1,606,615 - -0.1 1,606,615 - +0.3 1,606,615 - +0.0 1,606,658 + +0.9 1,606,698 + +0.1 1,606,698 + -0.2 1,606,722 - -0.0 1,606,783 + +0.1 1,606,801 + CA265_RS06495 0.11 +0.1 1,606,843 + CA265_RS06495 0.22 +1.0 1,606,868 + CA265_RS06495 0.28 +0.0 1,606,899 - CA265_RS06495 0.36 -0.9 1,606,900 + CA265_RS06495 0.36 -0.1 1,606,921 + CA265_RS06495 0.41 +0.1 1,606,949 + CA265_RS06495 0.48 -0.1 1,606,995 + CA265_RS06495 0.60 -0.2 1,606,996 - CA265_RS06495 0.60 -0.3 1,606,996 - CA265_RS06495 0.60 +0.1 1,606,996 - CA265_RS06495 0.60 +1.1 1,606,998 - CA265_RS06495 0.60 +0.6 1,606,998 - CA265_RS06495 0.60 -0.5 1,606,998 - CA265_RS06495 0.60 +0.4 1,607,038 + CA265_RS06495 0.70 -0.2 1,607,039 - CA265_RS06495 0.71 -0.0 1,607,120 + +0.5 1,607,162 + -0.2 1,607,246 + +0.4 1,607,247 - -0.1 1,607,255 + -0.1 1,607,256 - -0.4 1,607,336 + +0.2 1,607,340 + +0.9 1,607,340 + +0.0 1,607,340 + +0.9 1,607,551 + CA265_RS06500 0.36 -1.3 1,607,551 + CA265_RS06500 0.36 -0.5 1,607,552 - CA265_RS06500 0.37 +0.4 1,607,558 + CA265_RS06500 0.38 -1.8 1,607,559 - CA265_RS06500 0.38 -0.4 1,607,578 + CA265_RS06500 0.43 -0.5 1,607,625 - CA265_RS06500 0.54 +0.1 1,607,625 - CA265_RS06500 0.54 +1.8 1,607,631 - CA265_RS06500 0.55 -0.1 1,607,721 - CA265_RS06500 0.77 -1.0 1,607,776 + CA265_RS06500 0.90 -0.5 1,607,784 - +1.1 1,607,813 - -0.2 1,607,813 - +0.5 1,607,879 + -0.8 1,607,909 + -1.1 1,607,912 - -0.6 1,608,002 + +0.1 1,608,006 + -0.1 1,608,008 + -0.3 1,608,009 - +0.9 1,608,160 + -0.4 1,608,191 - CA265_RS06505 0.12 -0.9 1,608,282 - CA265_RS06505 0.38 -0.3 1,608,287 + CA265_RS06505 0.39 +0.7 1,608,333 + CA265_RS06505 0.52 -0.3 1,608,467 + +0.0 1,608,467 + +0.1 1,608,503 + -1.0 1,608,512 - +0.2 1,608,536 + +0.1 1,608,537 - -0.6 1,608,538 + +0.5 1,608,539 - +0.3 1,608,573 - +0.3 1,608,600 - +0.1 1,608,600 - -0.5 1,608,640 + -0.5 1,608,680 + -0.4 1,608,681 - +0.2 1,608,691 + -0.5 1,608,691 + -0.7 1,608,692 - -0.5 1,608,720 + -0.2
Or see this region's nucleotide sequence