Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04035 and CA265_RS04040 are separated by 36 nucleotides CA265_RS04040 and CA265_RS04045 are separated by 134 nucleotides CA265_RS04045 and CA265_RS04050 are separated by 66 nucleotides
CA265_RS04035: CA265_RS04035 - hypothetical protein, at 967,991 to 969,499
_RS04035
CA265_RS04040: CA265_RS04040 - hypothetical protein, at 969,536 to 969,853
_RS04040
CA265_RS04045: CA265_RS04045 - hypothetical protein, at 969,988 to 970,380
_RS04045
CA265_RS04050: CA265_RS04050 - nucleoside-diphosphate sugar epimerase, at 970,447 to 971,322
_RS04050
Position (kb)
969
970 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 968.559 kb on - strand, within CA265_RS04035 at 968.561 kb on - strand, within CA265_RS04035 at 968.561 kb on - strand, within CA265_RS04035 at 968.608 kb on + strand, within CA265_RS04035 at 968.677 kb on - strand, within CA265_RS04035 at 968.784 kb on + strand, within CA265_RS04035 at 968.872 kb on + strand, within CA265_RS04035 at 968.872 kb on + strand, within CA265_RS04035 at 968.885 kb on + strand, within CA265_RS04035 at 968.886 kb on - strand, within CA265_RS04035 at 968.886 kb on - strand, within CA265_RS04035 at 968.970 kb on + strand, within CA265_RS04035 at 968.987 kb on - strand, within CA265_RS04035 at 969.097 kb on + strand, within CA265_RS04035 at 969.098 kb on - strand, within CA265_RS04035 at 969.299 kb on + strand, within CA265_RS04035 at 969.369 kb on - strand at 969.374 kb on - strand at 969.479 kb on - strand at 969.543 kb on + strand at 969.584 kb on - strand, within CA265_RS04040 at 969.595 kb on + strand, within CA265_RS04040 at 969.596 kb on - strand, within CA265_RS04040 at 969.596 kb on - strand, within CA265_RS04040 at 969.600 kb on - strand, within CA265_RS04040 at 969.892 kb on - strand at 969.927 kb on + strand at 969.928 kb on - strand at 969.944 kb on - strand at 969.970 kb on - strand at 969.970 kb on - strand at 970.005 kb on + strand at 970.023 kb on - strand at 970.105 kb on + strand, within CA265_RS04045 at 970.106 kb on - strand, within CA265_RS04045 at 970.183 kb on - strand, within CA265_RS04045 at 970.216 kb on + strand, within CA265_RS04045 at 970.230 kb on - strand, within CA265_RS04045 at 970.256 kb on + strand, within CA265_RS04045 at 970.274 kb on + strand, within CA265_RS04045 at 970.275 kb on - strand, within CA265_RS04045 at 970.304 kb on + strand, within CA265_RS04045 at 970.378 kb on + strand at 970.378 kb on + strand at 970.379 kb on - strand at 970.396 kb on + strand at 970.780 kb on + strand, within CA265_RS04050 at 970.781 kb on - strand, within CA265_RS04050 at 970.800 kb on + strand, within CA265_RS04050 at 970.801 kb on - strand, within CA265_RS04050 at 970.801 kb on - strand, within CA265_RS04050 at 970.811 kb on - strand, within CA265_RS04050 at 970.852 kb on - strand, within CA265_RS04050
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 968,559 - CA265_RS04035 0.38 +0.4 968,561 - CA265_RS04035 0.38 -1.0 968,561 - CA265_RS04035 0.38 +0.0 968,608 + CA265_RS04035 0.41 -0.4 968,677 - CA265_RS04035 0.45 -0.3 968,784 + CA265_RS04035 0.53 +1.4 968,872 + CA265_RS04035 0.58 +0.4 968,872 + CA265_RS04035 0.58 -1.6 968,885 + CA265_RS04035 0.59 -0.5 968,886 - CA265_RS04035 0.59 -0.3 968,886 - CA265_RS04035 0.59 +0.0 968,970 + CA265_RS04035 0.65 +0.2 968,987 - CA265_RS04035 0.66 +0.8 969,097 + CA265_RS04035 0.73 -0.4 969,098 - CA265_RS04035 0.73 -0.5 969,299 + CA265_RS04035 0.87 -1.1 969,369 - +0.2 969,374 - +0.4 969,479 - +0.1 969,543 + -0.9 969,584 - CA265_RS04040 0.15 -1.6 969,595 + CA265_RS04040 0.19 -0.6 969,596 - CA265_RS04040 0.19 -1.6 969,596 - CA265_RS04040 0.19 +0.6 969,600 - CA265_RS04040 0.20 -0.5 969,892 - +0.5 969,927 + -0.4 969,928 - -0.1 969,944 - -1.0 969,970 - +0.7 969,970 - -0.7 970,005 + +1.9 970,023 - -0.2 970,105 + CA265_RS04045 0.30 +0.0 970,106 - CA265_RS04045 0.30 +0.7 970,183 - CA265_RS04045 0.50 -2.1 970,216 + CA265_RS04045 0.58 +0.7 970,230 - CA265_RS04045 0.62 +1.0 970,256 + CA265_RS04045 0.68 -1.5 970,274 + CA265_RS04045 0.73 -0.3 970,275 - CA265_RS04045 0.73 -1.0 970,304 + CA265_RS04045 0.80 +0.7 970,378 + +0.6 970,378 + -0.1 970,379 - -0.3 970,396 + -1.4 970,780 + CA265_RS04050 0.38 -0.4 970,781 - CA265_RS04050 0.38 -0.1 970,800 + CA265_RS04050 0.40 -0.3 970,801 - CA265_RS04050 0.40 -0.9 970,801 - CA265_RS04050 0.40 -0.6 970,811 - CA265_RS04050 0.42 +0.5 970,852 - CA265_RS04050 0.46 -0.1
Or see this region's nucleotide sequence