Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03825 and CA265_RS03830 are separated by 41 nucleotides CA265_RS03830 and CA265_RS03835 are separated by 41 nucleotides CA265_RS03835 and CA265_RS03840 are separated by 197 nucleotides CA265_RS03840 and CA265_RS03845 are separated by 237 nucleotides
CA265_RS03825: CA265_RS03825 - hypothetical protein, at 902,982 to 903,257
_RS03825
CA265_RS03830: CA265_RS03830 - DUF4833 domain-containing protein, at 903,299 to 903,877
_RS03830
CA265_RS03835: CA265_RS03835 - hypothetical protein, at 903,919 to 904,101
_RS03835
CA265_RS03840: CA265_RS03840 - tRNA-Cys, at 904,299 to 904,372
_RS03840
CA265_RS03845: CA265_RS03845 - TldD protein, at 904,610 to 906,250
_RS03845
Position (kb)
903
904
905 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 902.921 kb on - strand at 902.927 kb on - strand at 902.975 kb on + strand at 902.990 kb on + strand at 903.037 kb on + strand, within CA265_RS03825 at 903.038 kb on - strand, within CA265_RS03825 at 903.125 kb on - strand, within CA265_RS03825 at 903.150 kb on + strand, within CA265_RS03825 at 903.150 kb on + strand, within CA265_RS03825 at 903.156 kb on - strand, within CA265_RS03825 at 903.168 kb on - strand, within CA265_RS03825 at 903.187 kb on + strand, within CA265_RS03825 at 903.212 kb on + strand, within CA265_RS03825 at 903.255 kb on + strand at 903.325 kb on + strand at 903.371 kb on + strand, within CA265_RS03830 at 903.422 kb on - strand, within CA265_RS03830 at 903.422 kb on - strand, within CA265_RS03830 at 903.476 kb on - strand, within CA265_RS03830 at 903.478 kb on - strand, within CA265_RS03830 at 903.529 kb on - strand, within CA265_RS03830 at 903.584 kb on - strand, within CA265_RS03830 at 903.680 kb on + strand, within CA265_RS03830 at 903.687 kb on + strand, within CA265_RS03830 at 903.698 kb on - strand, within CA265_RS03830 at 903.698 kb on - strand, within CA265_RS03830 at 903.728 kb on + strand, within CA265_RS03830 at 903.729 kb on - strand, within CA265_RS03830 at 903.733 kb on + strand, within CA265_RS03830 at 903.734 kb on - strand, within CA265_RS03830 at 903.734 kb on - strand, within CA265_RS03830 at 903.880 kb on + strand at 903.931 kb on - strand at 903.986 kb on + strand, within CA265_RS03835 at 903.987 kb on - strand, within CA265_RS03835 at 904.094 kb on - strand at 904.101 kb on + strand at 904.101 kb on + strand at 904.102 kb on - strand at 904.119 kb on - strand at 904.210 kb on + strand at 904.210 kb on + strand at 904.236 kb on + strand at 904.237 kb on - strand at 904.273 kb on + strand at 904.290 kb on + strand at 904.290 kb on + strand at 904.375 kb on - strand at 904.375 kb on - strand at 904.385 kb on + strand at 904.385 kb on + strand at 904.386 kb on - strand at 904.386 kb on - strand at 904.386 kb on - strand at 904.388 kb on - strand at 904.498 kb on + strand at 904.498 kb on + strand at 904.507 kb on + strand at 904.565 kb on + strand at 904.565 kb on + strand at 904.566 kb on - strand at 904.634 kb on - strand at 904.637 kb on + strand at 904.637 kb on + strand at 904.837 kb on + strand, within CA265_RS03845 at 904.838 kb on - strand, within CA265_RS03845 at 904.874 kb on - strand, within CA265_RS03845 at 904.875 kb on + strand, within CA265_RS03845 at 904.918 kb on - strand, within CA265_RS03845 at 904.918 kb on - strand at 904.959 kb on - strand, within CA265_RS03845 at 904.959 kb on - strand, within CA265_RS03845
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 902,921 - +0.3 902,927 - -0.1 902,975 + -0.5 902,990 + +0.3 903,037 + CA265_RS03825 0.20 -0.1 903,038 - CA265_RS03825 0.20 +0.3 903,125 - CA265_RS03825 0.52 +0.0 903,150 + CA265_RS03825 0.61 +0.3 903,150 + CA265_RS03825 0.61 +0.2 903,156 - CA265_RS03825 0.63 +0.5 903,168 - CA265_RS03825 0.67 +0.4 903,187 + CA265_RS03825 0.74 +0.4 903,212 + CA265_RS03825 0.83 -0.4 903,255 + -0.7 903,325 + -2.2 903,371 + CA265_RS03830 0.12 -0.6 903,422 - CA265_RS03830 0.21 +0.9 903,422 - CA265_RS03830 0.21 -1.8 903,476 - CA265_RS03830 0.31 -0.6 903,478 - CA265_RS03830 0.31 +0.5 903,529 - CA265_RS03830 0.40 -1.0 903,584 - CA265_RS03830 0.49 +0.1 903,680 + CA265_RS03830 0.66 -0.6 903,687 + CA265_RS03830 0.67 -1.7 903,698 - CA265_RS03830 0.69 -0.9 903,698 - CA265_RS03830 0.69 -0.9 903,728 + CA265_RS03830 0.74 -0.1 903,729 - CA265_RS03830 0.74 -1.1 903,733 + CA265_RS03830 0.75 +0.2 903,734 - CA265_RS03830 0.75 -0.9 903,734 - CA265_RS03830 0.75 +0.3 903,880 + -0.2 903,931 - +0.7 903,986 + CA265_RS03835 0.37 -0.6 903,987 - CA265_RS03835 0.37 -0.4 904,094 - +0.3 904,101 + -1.6 904,101 + -0.2 904,102 - -0.4 904,119 - -0.0 904,210 + -0.8 904,210 + -0.0 904,236 + -0.6 904,237 - +0.2 904,273 + -0.6 904,290 + -0.1 904,290 + -0.1 904,375 - -1.5 904,375 - -0.7 904,385 + +0.1 904,385 + -0.9 904,386 - +0.6 904,386 - -0.3 904,386 - -0.3 904,388 - +0.0 904,498 + -0.4 904,498 + +2.2 904,507 + -0.9 904,565 + +1.4 904,565 + -0.4 904,566 - -0.2 904,634 - -0.1 904,637 + +0.6 904,637 + +0.2 904,837 + CA265_RS03845 0.14 +0.1 904,838 - CA265_RS03845 0.14 -0.3 904,874 - CA265_RS03845 0.16 +0.4 904,875 + CA265_RS03845 0.16 -1.1 904,918 - CA265_RS03845 0.19 +1.4 904,918 - -1.8 904,959 - CA265_RS03845 0.21 +0.2 904,959 - CA265_RS03845 0.21 -0.9
Or see this region's nucleotide sequence