Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03645 and CA265_RS03650 are separated by 70 nucleotides CA265_RS03650 and CA265_RS03655 are separated by 68 nucleotides CA265_RS03655 and CA265_RS03660 are separated by 29 nucleotides
CA265_RS03645: CA265_RS03645 - L,D-transpeptidase, at 870,738 to 872,300
_RS03645
CA265_RS03650: CA265_RS03650 - NUDIX hydrolase, at 872,371 to 873,063
_RS03650
CA265_RS03655: CA265_RS03655 - phosphoribosylpyrophosphate synthetase, at 873,132 to 873,965
_RS03655
CA265_RS03660: CA265_RS03660 - hypothetical protein, at 873,995 to 875,047
_RS03660
Position (kb)
873
874 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 872.139 kb on - strand, within CA265_RS03645 at 872.202 kb on + strand at 872.203 kb on - strand at 872.203 kb on - strand at 872.356 kb on + strand at 872.356 kb on + strand at 872.441 kb on + strand, within CA265_RS03650 at 872.472 kb on - strand, within CA265_RS03650 at 872.490 kb on - strand, within CA265_RS03650 at 872.534 kb on + strand, within CA265_RS03650 at 872.607 kb on + strand, within CA265_RS03650 at 872.608 kb on - strand, within CA265_RS03650 at 872.648 kb on - strand, within CA265_RS03650 at 872.660 kb on + strand, within CA265_RS03650 at 872.754 kb on + strand, within CA265_RS03650 at 872.826 kb on - strand, within CA265_RS03650 at 872.867 kb on - strand, within CA265_RS03650 at 872.950 kb on - strand, within CA265_RS03650 at 873.013 kb on + strand at 873.137 kb on - strand at 873.222 kb on - strand, within CA265_RS03655 at 873.261 kb on - strand, within CA265_RS03655 at 873.309 kb on - strand, within CA265_RS03655 at 873.311 kb on + strand, within CA265_RS03655 at 873.312 kb on - strand, within CA265_RS03655 at 873.312 kb on - strand, within CA265_RS03655 at 873.359 kb on + strand, within CA265_RS03655 at 873.366 kb on + strand, within CA265_RS03655 at 873.438 kb on - strand, within CA265_RS03655 at 873.441 kb on - strand, within CA265_RS03655 at 873.522 kb on - strand, within CA265_RS03655 at 873.553 kb on + strand, within CA265_RS03655 at 873.584 kb on + strand, within CA265_RS03655 at 873.584 kb on + strand, within CA265_RS03655 at 873.587 kb on - strand, within CA265_RS03655 at 873.656 kb on - strand, within CA265_RS03655 at 873.656 kb on - strand, within CA265_RS03655 at 873.710 kb on - strand, within CA265_RS03655 at 873.710 kb on - strand, within CA265_RS03655 at 873.710 kb on - strand, within CA265_RS03655 at 873.710 kb on - strand, within CA265_RS03655 at 873.749 kb on - strand, within CA265_RS03655 at 873.755 kb on + strand, within CA265_RS03655 at 873.826 kb on - strand, within CA265_RS03655 at 873.829 kb on + strand, within CA265_RS03655 at 873.829 kb on + strand, within CA265_RS03655 at 873.830 kb on - strand, within CA265_RS03655 at 873.899 kb on + strand at 873.943 kb on + strand at 873.969 kb on + strand at 873.974 kb on - strand at 873.982 kb on + strand at 873.983 kb on - strand at 874.033 kb on - strand at 874.083 kb on - strand at 874.109 kb on - strand, within CA265_RS03660 at 874.197 kb on - strand, within CA265_RS03660 at 874.217 kb on - strand, within CA265_RS03660 at 874.218 kb on + strand, within CA265_RS03660 at 874.219 kb on - strand, within CA265_RS03660 at 874.219 kb on - strand, within CA265_RS03660 at 874.275 kb on + strand, within CA265_RS03660 at 874.275 kb on + strand, within CA265_RS03660 at 874.276 kb on - strand, within CA265_RS03660 at 874.341 kb on + strand, within CA265_RS03660 at 874.405 kb on - strand, within CA265_RS03660 at 874.409 kb on + strand, within CA265_RS03660 at 874.421 kb on - strand, within CA265_RS03660 at 874.437 kb on - strand, within CA265_RS03660 at 874.439 kb on - strand, within CA265_RS03660 at 874.471 kb on + strand, within CA265_RS03660 at 874.508 kb on - strand, within CA265_RS03660 at 874.512 kb on - strand, within CA265_RS03660 at 874.512 kb on - strand, within CA265_RS03660 at 874.512 kb on - strand, within CA265_RS03660 at 874.549 kb on + strand, within CA265_RS03660 at 874.571 kb on - strand, within CA265_RS03660 at 874.658 kb on - strand, within CA265_RS03660 at 874.659 kb on + strand, within CA265_RS03660 at 874.685 kb on + strand, within CA265_RS03660 at 874.705 kb on - strand, within CA265_RS03660 at 874.725 kb on + strand, within CA265_RS03660 at 874.726 kb on - strand, within CA265_RS03660 at 874.752 kb on - strand, within CA265_RS03660 at 874.767 kb on + strand, within CA265_RS03660 at 874.792 kb on + strand, within CA265_RS03660 at 874.798 kb on + strand, within CA265_RS03660 at 874.824 kb on + strand, within CA265_RS03660 at 874.852 kb on + strand, within CA265_RS03660 at 874.853 kb on - strand, within CA265_RS03660 at 874.853 kb on - strand, within CA265_RS03660 at 874.855 kb on - strand, within CA265_RS03660 at 874.878 kb on + strand, within CA265_RS03660 at 874.879 kb on - strand, within CA265_RS03660 at 874.881 kb on + strand, within CA265_RS03660 at 874.882 kb on - strand, within CA265_RS03660 at 874.910 kb on - strand, within CA265_RS03660 at 874.929 kb on - strand, within CA265_RS03660 at 874.947 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 872,139 - CA265_RS03645 0.90 -1.4 872,202 + +0.8 872,203 - +0.1 872,203 - -0.2 872,356 + +0.5 872,356 + -0.5 872,441 + CA265_RS03650 0.10 -0.9 872,472 - CA265_RS03650 0.15 -0.6 872,490 - CA265_RS03650 0.17 +0.2 872,534 + CA265_RS03650 0.24 -0.2 872,607 + CA265_RS03650 0.34 +0.0 872,608 - CA265_RS03650 0.34 -0.4 872,648 - CA265_RS03650 0.40 -0.3 872,660 + CA265_RS03650 0.42 -0.4 872,754 + CA265_RS03650 0.55 -0.4 872,826 - CA265_RS03650 0.66 +0.0 872,867 - CA265_RS03650 0.72 +0.1 872,950 - CA265_RS03650 0.84 -0.3 873,013 + +0.0 873,137 - -0.0 873,222 - CA265_RS03655 0.11 -4.0 873,261 - CA265_RS03655 0.15 +0.7 873,309 - CA265_RS03655 0.21 -1.1 873,311 + CA265_RS03655 0.21 -0.9 873,312 - CA265_RS03655 0.22 -0.6 873,312 - CA265_RS03655 0.22 +1.2 873,359 + CA265_RS03655 0.27 -0.5 873,366 + CA265_RS03655 0.28 -1.0 873,438 - CA265_RS03655 0.37 -0.4 873,441 - CA265_RS03655 0.37 +0.4 873,522 - CA265_RS03655 0.47 -1.1 873,553 + CA265_RS03655 0.50 -1.6 873,584 + CA265_RS03655 0.54 -0.4 873,584 + CA265_RS03655 0.54 -0.4 873,587 - CA265_RS03655 0.55 -0.1 873,656 - CA265_RS03655 0.63 -1.5 873,656 - CA265_RS03655 0.63 -1.1 873,710 - CA265_RS03655 0.69 -1.0 873,710 - CA265_RS03655 0.69 -0.7 873,710 - CA265_RS03655 0.69 -1.0 873,710 - CA265_RS03655 0.69 -0.6 873,749 - CA265_RS03655 0.74 -1.0 873,755 + CA265_RS03655 0.75 -0.5 873,826 - CA265_RS03655 0.83 -0.9 873,829 + CA265_RS03655 0.84 -0.7 873,829 + CA265_RS03655 0.84 +0.2 873,830 - CA265_RS03655 0.84 -1.4 873,899 + -1.5 873,943 + -0.3 873,969 + -0.5 873,974 - +0.0 873,982 + +0.9 873,983 - -0.7 874,033 - -0.1 874,083 - +0.2 874,109 - CA265_RS03660 0.11 +0.5 874,197 - CA265_RS03660 0.19 +0.3 874,217 - CA265_RS03660 0.21 -0.1 874,218 + CA265_RS03660 0.21 +0.3 874,219 - CA265_RS03660 0.21 -0.8 874,219 - CA265_RS03660 0.21 +0.7 874,275 + CA265_RS03660 0.27 +0.4 874,275 + CA265_RS03660 0.27 +0.9 874,276 - CA265_RS03660 0.27 +1.2 874,341 + CA265_RS03660 0.33 -0.3 874,405 - CA265_RS03660 0.39 -1.9 874,409 + CA265_RS03660 0.39 +1.3 874,421 - CA265_RS03660 0.40 +0.0 874,437 - CA265_RS03660 0.42 +1.0 874,439 - CA265_RS03660 0.42 +0.2 874,471 + CA265_RS03660 0.45 +1.1 874,508 - CA265_RS03660 0.49 +1.0 874,512 - CA265_RS03660 0.49 +0.2 874,512 - CA265_RS03660 0.49 -1.0 874,512 - CA265_RS03660 0.49 +0.3 874,549 + CA265_RS03660 0.53 -0.2 874,571 - CA265_RS03660 0.55 +0.9 874,658 - CA265_RS03660 0.63 +0.4 874,659 + CA265_RS03660 0.63 -0.1 874,685 + CA265_RS03660 0.66 +0.6 874,705 - CA265_RS03660 0.67 -0.0 874,725 + CA265_RS03660 0.69 +0.6 874,726 - CA265_RS03660 0.69 +1.2 874,752 - CA265_RS03660 0.72 -0.1 874,767 + CA265_RS03660 0.73 -2.8 874,792 + CA265_RS03660 0.76 +0.0 874,798 + CA265_RS03660 0.76 +0.2 874,824 + CA265_RS03660 0.79 -0.8 874,852 + CA265_RS03660 0.81 -0.6 874,853 - CA265_RS03660 0.81 +0.6 874,853 - CA265_RS03660 0.81 -0.0 874,855 - CA265_RS03660 0.82 -0.8 874,878 + CA265_RS03660 0.84 +0.1 874,879 - CA265_RS03660 0.84 -0.4 874,881 + CA265_RS03660 0.84 +1.0 874,882 - CA265_RS03660 0.84 +0.2 874,910 - CA265_RS03660 0.87 -0.2 874,929 - CA265_RS03660 0.89 +0.6 874,947 - +0.6
Or see this region's nucleotide sequence