Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02755 and CA265_RS02760 are separated by 31 nucleotides CA265_RS02760 and CA265_RS02765 are separated by 33 nucleotides CA265_RS02765 and CA265_RS02770 are separated by 332 nucleotides
CA265_RS02755: CA265_RS02755 - phenylalanine--tRNA ligase subunit beta, at 691,675 to 694,074
_RS02755
CA265_RS02760: CA265_RS02760 - hypothetical protein, at 694,106 to 694,396
_RS02760
CA265_RS02765: CA265_RS02765 - cell division protein ZapA, at 694,430 to 694,720
_RS02765
CA265_RS02770: CA265_RS02770 - ribonuclease Y, at 695,053 to 696,624
_RS02770
Position (kb) 694
695 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 694.099 kb on + strand at 694.132 kb on + strand at 694.137 kb on + strand, within CA265_RS02760 at 694.167 kb on + strand, within CA265_RS02760 at 694.171 kb on + strand, within CA265_RS02760 at 694.300 kb on + strand, within CA265_RS02760 at 694.418 kb on + strand at 694.472 kb on + strand, within CA265_RS02765 at 694.558 kb on + strand, within CA265_RS02765 at 694.610 kb on + strand, within CA265_RS02765 at 694.644 kb on + strand, within CA265_RS02765 at 694.695 kb on + strand at 694.702 kb on + strand at 694.738 kb on + strand at 694.772 kb on + strand at 694.810 kb on + strand at 694.918 kb on + strand at 694.918 kb on + strand at 694.948 kb on + strand at 694.979 kb on + strand at 694.979 kb on + strand at 694.979 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 694,099 + -1.7 694,132 + -1.1 694,137 + CA265_RS02760 0.11 -2.9 694,167 + CA265_RS02760 0.21 -0.5 694,171 + CA265_RS02760 0.22 -2.3 694,300 + CA265_RS02760 0.67 -1.4 694,418 + -1.9 694,472 + CA265_RS02765 0.14 +0.9 694,558 + CA265_RS02765 0.44 -1.5 694,610 + CA265_RS02765 0.62 -1.3 694,644 + CA265_RS02765 0.74 -0.9 694,695 + +0.0 694,702 + +1.1 694,738 + -0.2 694,772 + +0.3 694,810 + -0.3 694,918 + -1.3 694,918 + -0.2 694,948 + +0.1 694,979 + +0.4 694,979 + -0.5 694,979 + -0.2
Or see this region's nucleotide sequence