Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01520 and CA265_RS01525 are separated by 152 nucleotides CA265_RS01525 and CA265_RS01530 are separated by 536 nucleotides
CA265_RS01520: CA265_RS01520 - beta-galactosidase, at 388,813 to 390,663
_RS01520
CA265_RS01525: CA265_RS01525 - hypothetical protein, at 390,816 to 391,262
_RS01525
CA265_RS01530: CA265_RS01530 - DUF5107 domain-containing protein, at 391,799 to 394,906
_RS01530
Position (kb)
390
391
392 Strain fitness (log2 ratio)
-2
-1
0
1 at 389.917 kb on - strand, within CA265_RS01520 at 389.917 kb on - strand, within CA265_RS01520 at 389.919 kb on + strand, within CA265_RS01520 at 389.937 kb on + strand, within CA265_RS01520 at 389.942 kb on + strand, within CA265_RS01520 at 390.097 kb on + strand, within CA265_RS01520 at 390.242 kb on + strand, within CA265_RS01520 at 390.301 kb on + strand, within CA265_RS01520 at 390.302 kb on - strand, within CA265_RS01520 at 390.331 kb on + strand, within CA265_RS01520 at 390.332 kb on - strand, within CA265_RS01520 at 390.466 kb on + strand, within CA265_RS01520 at 390.467 kb on - strand, within CA265_RS01520 at 390.574 kb on + strand at 390.574 kb on + strand at 390.574 kb on + strand at 390.575 kb on - strand at 390.621 kb on - strand at 390.639 kb on + strand at 390.642 kb on + strand at 390.672 kb on - strand at 390.730 kb on + strand at 390.730 kb on + strand at 390.732 kb on + strand at 390.740 kb on - strand at 390.740 kb on - strand at 390.803 kb on - strand at 391.010 kb on - strand, within CA265_RS01525 at 391.023 kb on - strand, within CA265_RS01525 at 391.078 kb on - strand, within CA265_RS01525 at 391.085 kb on + strand, within CA265_RS01525 at 391.186 kb on + strand, within CA265_RS01525 at 391.187 kb on - strand, within CA265_RS01525 at 391.212 kb on + strand, within CA265_RS01525 at 391.236 kb on - strand at 391.236 kb on - strand at 391.238 kb on + strand at 391.243 kb on - strand at 391.267 kb on + strand at 391.283 kb on - strand at 391.329 kb on - strand at 391.329 kb on - strand at 391.367 kb on - strand at 391.408 kb on - strand at 391.408 kb on - strand at 391.446 kb on + strand at 391.526 kb on - strand at 391.531 kb on + strand at 391.531 kb on + strand at 391.531 kb on + strand at 391.531 kb on + strand at 391.532 kb on - strand at 391.532 kb on - strand at 391.547 kb on - strand at 391.557 kb on - strand at 391.564 kb on + strand at 391.598 kb on - strand at 391.667 kb on - strand at 391.671 kb on + strand at 391.676 kb on - strand at 391.695 kb on + strand at 391.734 kb on - strand at 391.734 kb on - strand at 391.737 kb on + strand at 391.739 kb on + strand at 391.740 kb on - strand at 391.740 kb on - strand at 391.777 kb on - strand at 391.796 kb on - strand at 391.885 kb on + strand at 391.886 kb on - strand at 391.949 kb on + strand at 391.950 kb on - strand at 391.950 kb on - strand at 391.955 kb on + strand at 391.956 kb on - strand at 392.009 kb on - strand at 392.028 kb on - strand at 392.031 kb on + strand at 392.067 kb on + strand at 392.138 kb on - strand, within CA265_RS01530 at 392.139 kb on + strand, within CA265_RS01530 at 392.139 kb on + strand, within CA265_RS01530 at 392.140 kb on - strand, within CA265_RS01530 at 392.183 kb on + strand, within CA265_RS01530 at 392.191 kb on + strand, within CA265_RS01530 at 392.200 kb on + strand, within CA265_RS01530 at 392.228 kb on + strand, within CA265_RS01530 at 392.259 kb on + strand, within CA265_RS01530 at 392.259 kb on + strand, within CA265_RS01530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 389,917 - CA265_RS01520 0.60 +0.1 389,917 - CA265_RS01520 0.60 -0.1 389,919 + CA265_RS01520 0.60 -0.2 389,937 + CA265_RS01520 0.61 -1.6 389,942 + CA265_RS01520 0.61 -2.1 390,097 + CA265_RS01520 0.69 -0.2 390,242 + CA265_RS01520 0.77 -0.4 390,301 + CA265_RS01520 0.80 -1.5 390,302 - CA265_RS01520 0.80 -0.2 390,331 + CA265_RS01520 0.82 +0.8 390,332 - CA265_RS01520 0.82 -1.2 390,466 + CA265_RS01520 0.89 +0.2 390,467 - CA265_RS01520 0.89 +0.2 390,574 + +0.1 390,574 + -1.1 390,574 + -0.3 390,575 - -0.5 390,621 - +0.9 390,639 + +0.2 390,642 + -0.1 390,672 - +0.9 390,730 + -0.1 390,730 + -1.8 390,732 + -1.4 390,740 - +0.1 390,740 - +0.7 390,803 - -0.6 391,010 - CA265_RS01525 0.43 +0.9 391,023 - CA265_RS01525 0.46 -0.6 391,078 - CA265_RS01525 0.59 +0.5 391,085 + CA265_RS01525 0.60 -0.3 391,186 + CA265_RS01525 0.83 -1.1 391,187 - CA265_RS01525 0.83 -0.6 391,212 + CA265_RS01525 0.89 -0.9 391,236 - -0.0 391,236 - -0.7 391,238 + +0.5 391,243 - -1.5 391,267 + -0.1 391,283 - -0.1 391,329 - +0.4 391,329 - +0.1 391,367 - +0.0 391,408 - -1.2 391,408 - +0.9 391,446 + -0.6 391,526 - +0.3 391,531 + -0.2 391,531 + -0.7 391,531 + -0.5 391,531 + -0.1 391,532 - -0.1 391,532 - +0.3 391,547 - -0.5 391,557 - +0.7 391,564 + +0.7 391,598 - +0.0 391,667 - -0.9 391,671 + +0.0 391,676 - +0.2 391,695 + +0.2 391,734 - -0.5 391,734 - -0.4 391,737 + +0.5 391,739 + -0.0 391,740 - -0.8 391,740 - -0.7 391,777 - -0.7 391,796 - -0.4 391,885 + -0.6 391,886 - +0.2 391,949 + -0.2 391,950 - +0.7 391,950 - -0.5 391,955 + +0.1 391,956 - -0.4 392,009 - -0.1 392,028 - -0.6 392,031 + -1.1 392,067 + -0.2 392,138 - CA265_RS01530 0.11 -1.5 392,139 + CA265_RS01530 0.11 +0.3 392,139 + CA265_RS01530 0.11 +0.3 392,140 - CA265_RS01530 0.11 -0.0 392,183 + CA265_RS01530 0.12 -0.0 392,191 + CA265_RS01530 0.13 -0.3 392,200 + CA265_RS01530 0.13 -0.2 392,228 + CA265_RS01530 0.14 -0.0 392,259 + CA265_RS01530 0.15 -0.9 392,259 + CA265_RS01530 0.15 +0.3
Or see this region's nucleotide sequence