Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01315 and CA265_RS01320 are separated by 33 nucleotides CA265_RS01320 and CA265_RS01325 are separated by 239 nucleotides
CA265_RS01315: CA265_RS01315 - alpha-1,3-galactosidase B, at 331,823 to 333,628
_RS01315
CA265_RS01320: CA265_RS01320 - hypothetical protein, at 333,662 to 334,006
_RS01320
CA265_RS01325: CA265_RS01325 - hypothetical protein, at 334,246 to 335,121
_RS01325
Position (kb)
333
334
335 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 332.665 kb on + strand, within CA265_RS01315 at 332.665 kb on + strand, within CA265_RS01315 at 332.764 kb on + strand, within CA265_RS01315 at 332.810 kb on - strand, within CA265_RS01315 at 332.814 kb on + strand, within CA265_RS01315 at 332.814 kb on + strand, within CA265_RS01315 at 332.874 kb on - strand, within CA265_RS01315 at 332.874 kb on - strand, within CA265_RS01315 at 332.874 kb on - strand, within CA265_RS01315 at 332.875 kb on + strand, within CA265_RS01315 at 332.925 kb on + strand, within CA265_RS01315 at 332.933 kb on - strand, within CA265_RS01315 at 333.028 kb on + strand, within CA265_RS01315 at 333.064 kb on - strand, within CA265_RS01315 at 333.149 kb on - strand, within CA265_RS01315 at 333.214 kb on + strand, within CA265_RS01315 at 333.215 kb on - strand, within CA265_RS01315 at 333.286 kb on - strand, within CA265_RS01315 at 333.302 kb on - strand, within CA265_RS01315 at 333.325 kb on + strand, within CA265_RS01315 at 333.326 kb on - strand, within CA265_RS01315 at 333.329 kb on + strand, within CA265_RS01315 at 333.329 kb on + strand, within CA265_RS01315 at 333.329 kb on + strand, within CA265_RS01315 at 333.349 kb on + strand, within CA265_RS01315 at 333.496 kb on + strand at 333.586 kb on + strand at 333.607 kb on - strand at 333.607 kb on - strand at 333.646 kb on - strand at 333.664 kb on - strand at 333.668 kb on - strand at 333.712 kb on + strand, within CA265_RS01320 at 333.713 kb on - strand, within CA265_RS01320 at 333.773 kb on - strand, within CA265_RS01320 at 333.807 kb on - strand, within CA265_RS01320 at 334.013 kb on + strand at 334.016 kb on - strand at 334.096 kb on - strand at 334.134 kb on - strand at 334.231 kb on + strand at 334.241 kb on - strand at 334.262 kb on + strand at 334.327 kb on - strand at 334.329 kb on - strand at 334.350 kb on - strand, within CA265_RS01325 at 334.350 kb on - strand, within CA265_RS01325 at 334.423 kb on - strand, within CA265_RS01325 at 334.547 kb on + strand, within CA265_RS01325 at 334.563 kb on + strand, within CA265_RS01325 at 334.564 kb on - strand, within CA265_RS01325 at 334.638 kb on + strand, within CA265_RS01325 at 334.677 kb on - strand, within CA265_RS01325 at 334.773 kb on + strand, within CA265_RS01325 at 334.800 kb on + strand, within CA265_RS01325 at 334.801 kb on - strand, within CA265_RS01325 at 334.801 kb on - strand, within CA265_RS01325 at 334.818 kb on + strand, within CA265_RS01325 at 334.825 kb on - strand, within CA265_RS01325 at 334.880 kb on + strand, within CA265_RS01325 at 334.906 kb on + strand, within CA265_RS01325 at 334.907 kb on - strand, within CA265_RS01325 at 334.914 kb on + strand, within CA265_RS01325 at 334.914 kb on + strand, within CA265_RS01325 at 334.915 kb on - strand, within CA265_RS01325 at 334.931 kb on + strand, within CA265_RS01325
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 332,665 + CA265_RS01315 0.47 -0.9 332,665 + CA265_RS01315 0.47 -0.7 332,764 + CA265_RS01315 0.52 +2.2 332,810 - CA265_RS01315 0.55 +0.7 332,814 + CA265_RS01315 0.55 -0.3 332,814 + CA265_RS01315 0.55 -0.3 332,874 - CA265_RS01315 0.58 +0.1 332,874 - CA265_RS01315 0.58 -0.2 332,874 - CA265_RS01315 0.58 -0.1 332,875 + CA265_RS01315 0.58 +0.2 332,925 + CA265_RS01315 0.61 -1.0 332,933 - CA265_RS01315 0.61 +0.1 333,028 + CA265_RS01315 0.67 -0.7 333,064 - CA265_RS01315 0.69 -0.1 333,149 - CA265_RS01315 0.73 -0.3 333,214 + CA265_RS01315 0.77 +0.3 333,215 - CA265_RS01315 0.77 +0.1 333,286 - CA265_RS01315 0.81 +0.9 333,302 - CA265_RS01315 0.82 -0.1 333,325 + CA265_RS01315 0.83 -1.2 333,326 - CA265_RS01315 0.83 -0.7 333,329 + CA265_RS01315 0.83 -2.1 333,329 + CA265_RS01315 0.83 +0.1 333,329 + CA265_RS01315 0.83 +0.6 333,349 + CA265_RS01315 0.84 -0.2 333,496 + -0.2 333,586 + -1.9 333,607 - +0.5 333,607 - -0.0 333,646 - +0.6 333,664 - -0.2 333,668 - +0.4 333,712 + CA265_RS01320 0.14 -0.2 333,713 - CA265_RS01320 0.15 -0.2 333,773 - CA265_RS01320 0.32 -0.7 333,807 - CA265_RS01320 0.42 -0.6 334,013 + +0.5 334,016 - +0.5 334,096 - +1.2 334,134 - -0.4 334,231 + +2.3 334,241 - +0.5 334,262 + -0.2 334,327 - -0.1 334,329 - -1.1 334,350 - CA265_RS01325 0.12 -0.7 334,350 - CA265_RS01325 0.12 +0.3 334,423 - CA265_RS01325 0.20 +0.1 334,547 + CA265_RS01325 0.34 -0.2 334,563 + CA265_RS01325 0.36 -0.8 334,564 - CA265_RS01325 0.36 +1.0 334,638 + CA265_RS01325 0.45 -0.8 334,677 - CA265_RS01325 0.49 +0.1 334,773 + CA265_RS01325 0.60 +0.3 334,800 + CA265_RS01325 0.63 +0.8 334,801 - CA265_RS01325 0.63 +0.2 334,801 - CA265_RS01325 0.63 +0.1 334,818 + CA265_RS01325 0.65 -0.7 334,825 - CA265_RS01325 0.66 -0.8 334,880 + CA265_RS01325 0.72 +0.2 334,906 + CA265_RS01325 0.75 +0.3 334,907 - CA265_RS01325 0.75 -0.0 334,914 + CA265_RS01325 0.76 -0.1 334,914 + CA265_RS01325 0.76 +0.2 334,915 - CA265_RS01325 0.76 +0.1 334,931 + CA265_RS01325 0.78 -1.7
Or see this region's nucleotide sequence