Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2123 and Shewana3_2124 are separated by 212 nucleotides Shewana3_2124 and Shewana3_2125 are separated by 125 nucleotides Shewana3_2125 and Shewana3_2126 are separated by 110 nucleotides
Shewana3_2123: Shewana3_2123 - hypothetical protein (RefSeq), at 2,548,330 to 2,549,217
_2123
Shewana3_2124: Shewana3_2124 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 2,549,430 to 2,550,440
_2124
Shewana3_2125: Shewana3_2125 - superoxide dismutase, copper/zinc binding (RefSeq), at 2,550,566 to 2,551,078
_2125
Shewana3_2126: Shewana3_2126 - integrase catalytic subunit (RefSeq), at 2,551,189 to 2,551,911
_2126
Position (kb)
2549
2550
2551 Strain fitness (log2 ratio)
-2
-1
0
1 at 2548.790 kb on + strand, within Shewana3_2123 at 2548.939 kb on - strand, within Shewana3_2123 at 2549.080 kb on + strand, within Shewana3_2123 at 2549.080 kb on + strand, within Shewana3_2123 at 2549.238 kb on + strand at 2550.904 kb on + strand, within Shewana3_2125 at 2551.029 kb on - strand at 2551.145 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 2,548,790 + Shewana3_2123 0.52 -0.3 2,548,939 - Shewana3_2123 0.69 -2.6 2,549,080 + Shewana3_2123 0.84 -1.4 2,549,080 + Shewana3_2123 0.84 +0.4 2,549,238 + -1.8 2,550,904 + Shewana3_2125 0.66 +1.3 2,551,029 - +0.1 2,551,145 - -1.5
Or see this region's nucleotide sequence