Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0481 and Shewana3_0482 are separated by 45 nucleotides Shewana3_0482 and Shewana3_0483 are separated by 157 nucleotides Shewana3_0483 and Shewana3_0484 overlap by 4 nucleotides Shewana3_0484 and Shewana3_0485 are separated by 44 nucleotides
Shewana3_0481: Shewana3_0481 - response regulator receiver protein (RefSeq), at 562,825 to 564,396
_0481
Shewana3_0482: Shewana3_0482 - peptidase S9 prolyl oligopeptidase (RefSeq), at 564,442 to 566,502
_0482
Shewana3_0483: Shewana3_0483 - hypothetical protein (RefSeq), at 566,660 to 567,178
_0483
Shewana3_0484: Shewana3_0484 - XRE family transcriptional regulator (RefSeq), at 567,175 to 567,408
_0484
Shewana3_0485: Shewana3_0485 - hypothetical protein (RefSeq), at 567,453 to 568,031
_0485
Position (kb) 564
565
566
567 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 563.445 kb on + strand, within Shewana3_0481 at 563.649 kb on + strand, within Shewana3_0481 at 563.793 kb on + strand, within Shewana3_0481 at 563.851 kb on - strand, within Shewana3_0481 at 563.984 kb on - strand, within Shewana3_0481 at 563.984 kb on - strand, within Shewana3_0481 at 563.995 kb on + strand, within Shewana3_0481 at 564.166 kb on - strand, within Shewana3_0481 at 564.240 kb on + strand at 564.240 kb on + strand at 564.395 kb on + strand at 564.408 kb on - strand at 564.420 kb on + strand at 564.611 kb on + strand at 564.952 kb on - strand, within Shewana3_0482 at 565.043 kb on + strand, within Shewana3_0482 at 565.144 kb on - strand, within Shewana3_0482 at 565.144 kb on - strand, within Shewana3_0482 at 565.144 kb on - strand, within Shewana3_0482 at 565.144 kb on - strand, within Shewana3_0482 at 565.167 kb on - strand, within Shewana3_0482 at 565.589 kb on + strand, within Shewana3_0482 at 565.616 kb on + strand, within Shewana3_0482 at 565.632 kb on - strand, within Shewana3_0482 at 565.721 kb on - strand, within Shewana3_0482 at 565.877 kb on + strand, within Shewana3_0482 at 565.887 kb on + strand, within Shewana3_0482 at 566.017 kb on - strand, within Shewana3_0482 at 566.032 kb on + strand, within Shewana3_0482 at 566.040 kb on - strand, within Shewana3_0482 at 566.062 kb on - strand, within Shewana3_0482 at 566.077 kb on + strand, within Shewana3_0482 at 566.094 kb on + strand, within Shewana3_0482 at 566.160 kb on - strand, within Shewana3_0482 at 566.164 kb on + strand, within Shewana3_0482 at 566.470 kb on + strand at 566.632 kb on - strand at 566.642 kb on - strand at 567.130 kb on + strand at 567.243 kb on + strand, within Shewana3_0484 at 567.251 kb on - strand, within Shewana3_0484 at 567.251 kb on - strand, within Shewana3_0484 at 567.251 kb on - strand, within Shewana3_0484 at 567.297 kb on + strand, within Shewana3_0484 at 567.415 kb on + strand at 567.415 kb on + strand at 567.415 kb on + strand at 567.422 kb on + strand at 567.484 kb on + strand at 567.497 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 563,445 + Shewana3_0481 0.39 -1.2 563,649 + Shewana3_0481 0.52 +0.4 563,793 + Shewana3_0481 0.62 +0.3 563,851 - Shewana3_0481 0.65 -2.7 563,984 - Shewana3_0481 0.74 -3.0 563,984 - Shewana3_0481 0.74 +0.6 563,995 + Shewana3_0481 0.74 +0.8 564,166 - Shewana3_0481 0.85 -1.0 564,240 + +0.2 564,240 + -1.4 564,395 + +0.3 564,408 - +0.1 564,420 + -0.2 564,611 + -2.2 564,952 - Shewana3_0482 0.25 -2.8 565,043 + Shewana3_0482 0.29 -0.7 565,144 - Shewana3_0482 0.34 +0.3 565,144 - Shewana3_0482 0.34 +0.0 565,144 - Shewana3_0482 0.34 +1.3 565,144 - Shewana3_0482 0.34 +1.6 565,167 - Shewana3_0482 0.35 +1.5 565,589 + Shewana3_0482 0.56 -1.1 565,616 + Shewana3_0482 0.57 -1.6 565,632 - Shewana3_0482 0.58 +0.7 565,721 - Shewana3_0482 0.62 +0.4 565,877 + Shewana3_0482 0.70 +0.5 565,887 + Shewana3_0482 0.70 -1.0 566,017 - Shewana3_0482 0.76 +1.0 566,032 + Shewana3_0482 0.77 -1.0 566,040 - Shewana3_0482 0.78 +0.4 566,062 - Shewana3_0482 0.79 +2.9 566,077 + Shewana3_0482 0.79 -2.3 566,094 + Shewana3_0482 0.80 +0.2 566,160 - Shewana3_0482 0.83 -0.7 566,164 + Shewana3_0482 0.84 +0.2 566,470 + -1.7 566,632 - -0.6 566,642 - -0.8 567,130 + -1.1 567,243 + Shewana3_0484 0.29 +0.4 567,251 - Shewana3_0484 0.32 +1.4 567,251 - Shewana3_0484 0.32 +0.5 567,251 - Shewana3_0484 0.32 +0.7 567,297 + Shewana3_0484 0.52 -1.0 567,415 + +1.8 567,415 + +0.2 567,415 + +0.4 567,422 + -1.7 567,484 + +0.9 567,497 - +0.3
Or see this region's nucleotide sequence