Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purH and zntR are separated by 336 nucleotides zntR and Shewana3_0443 are separated by 2 nucleotides Shewana3_0443 and Shewana3_0444 are separated by 107 nucleotides
Shewana3_0441: purH - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (RefSeq), at 515,787 to 517,385
purH
Shewana3_0442: zntR - zinc-responsive transcriptional regulator (RefSeq), at 517,722 to 518,195
zntR
Shewana3_0443: Shewana3_0443 - permease (RefSeq), at 518,198 to 519,691
_0443
Shewana3_0444: Shewana3_0444 - band 7 protein (RefSeq), at 519,799 to 520,686
_0444
Position (kb) 518
519
520 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 517.226 kb on + strand at 517.385 kb on + strand at 517.566 kb on + strand at 517.640 kb on + strand at 517.648 kb on - strand at 517.679 kb on + strand at 517.713 kb on + strand at 517.714 kb on + strand at 517.779 kb on - strand, within zntR at 517.807 kb on - strand, within zntR at 517.835 kb on + strand, within zntR at 517.843 kb on - strand, within zntR at 517.952 kb on + strand, within zntR at 518.115 kb on - strand, within zntR at 518.147 kb on - strand, within zntR at 518.245 kb on + strand at 518.375 kb on - strand, within Shewana3_0443 at 518.411 kb on - strand, within Shewana3_0443 at 518.482 kb on - strand, within Shewana3_0443 at 518.580 kb on + strand, within Shewana3_0443 at 518.669 kb on + strand, within Shewana3_0443 at 518.683 kb on - strand, within Shewana3_0443 at 518.712 kb on + strand, within Shewana3_0443 at 518.862 kb on + strand, within Shewana3_0443 at 518.862 kb on + strand, within Shewana3_0443 at 518.911 kb on + strand, within Shewana3_0443 at 518.934 kb on - strand, within Shewana3_0443 at 518.954 kb on + strand, within Shewana3_0443 at 519.038 kb on - strand, within Shewana3_0443 at 519.088 kb on + strand, within Shewana3_0443 at 519.103 kb on + strand, within Shewana3_0443 at 519.233 kb on - strand, within Shewana3_0443 at 519.241 kb on + strand, within Shewana3_0443 at 519.251 kb on + strand, within Shewana3_0443 at 519.295 kb on + strand, within Shewana3_0443 at 519.305 kb on + strand, within Shewana3_0443 at 519.372 kb on + strand, within Shewana3_0443 at 519.420 kb on + strand, within Shewana3_0443 at 519.445 kb on + strand, within Shewana3_0443 at 519.457 kb on + strand, within Shewana3_0443 at 519.479 kb on + strand, within Shewana3_0443 at 519.479 kb on + strand, within Shewana3_0443 at 519.487 kb on - strand, within Shewana3_0443 at 519.679 kb on + strand at 519.745 kb on + strand at 519.859 kb on - strand at 519.980 kb on + strand, within Shewana3_0444 at 519.980 kb on + strand, within Shewana3_0444 at 520.111 kb on + strand, within Shewana3_0444 at 520.441 kb on + strand, within Shewana3_0444
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 517,226 + -3.6 517,385 + -5.8 517,566 + -1.8 517,640 + +0.4 517,648 - +1.7 517,679 + +0.7 517,713 + +1.0 517,714 + +1.7 517,779 - zntR Shewana3_0442 0.12 +0.1 517,807 - zntR Shewana3_0442 0.18 -0.5 517,835 + zntR Shewana3_0442 0.24 +1.0 517,843 - zntR Shewana3_0442 0.26 -0.3 517,952 + zntR Shewana3_0442 0.49 -1.3 518,115 - zntR Shewana3_0442 0.83 +0.7 518,147 - zntR Shewana3_0442 0.90 +1.6 518,245 + -1.2 518,375 - Shewana3_0443 0.12 +1.2 518,411 - Shewana3_0443 0.14 +1.4 518,482 - Shewana3_0443 0.19 +1.2 518,580 + Shewana3_0443 0.26 +1.4 518,669 + Shewana3_0443 0.32 +0.6 518,683 - Shewana3_0443 0.32 +1.0 518,712 + Shewana3_0443 0.34 +1.8 518,862 + Shewana3_0443 0.44 -0.9 518,862 + Shewana3_0443 0.44 +1.9 518,911 + Shewana3_0443 0.48 -2.1 518,934 - Shewana3_0443 0.49 -0.1 518,954 + Shewana3_0443 0.51 +0.8 519,038 - Shewana3_0443 0.56 +2.9 519,088 + Shewana3_0443 0.60 -0.5 519,103 + Shewana3_0443 0.61 +1.6 519,233 - Shewana3_0443 0.69 +1.8 519,241 + Shewana3_0443 0.70 -0.5 519,251 + Shewana3_0443 0.70 -0.8 519,295 + Shewana3_0443 0.73 +0.2 519,305 + Shewana3_0443 0.74 +0.6 519,372 + Shewana3_0443 0.79 -0.4 519,420 + Shewana3_0443 0.82 -0.7 519,445 + Shewana3_0443 0.83 +1.2 519,457 + Shewana3_0443 0.84 +2.0 519,479 + Shewana3_0443 0.86 +2.0 519,479 + Shewana3_0443 0.86 +3.1 519,487 - Shewana3_0443 0.86 -1.3 519,679 + -1.3 519,745 + -1.7 519,859 - +0.7 519,980 + Shewana3_0444 0.20 +0.2 519,980 + Shewana3_0444 0.20 +2.0 520,111 + Shewana3_0444 0.35 -0.8 520,441 + Shewana3_0444 0.72 +2.1
Or see this region's nucleotide sequence