Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0432 and Shewana3_0433 are separated by 359 nucleotides Shewana3_0433 and Shewana3_0434 are separated by 174 nucleotides
Shewana3_0432: Shewana3_0432 - 2-deoxyglucose-6-phosphatase (RefSeq), at 500,896 to 501,552
_0432
Shewana3_0433: Shewana3_0433 - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase (RefSeq), at 501,912 to 504,584
_0433
Shewana3_0434: Shewana3_0434 - anti-RNA polymerase sigma 70 factor (RefSeq), at 504,759 to 505,256
_0434
Position (kb) 501
502
503
504
505 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 501.064 kb on - strand, within Shewana3_0432 at 501.124 kb on + strand, within Shewana3_0432 at 501.149 kb on + strand, within Shewana3_0432 at 501.157 kb on - strand, within Shewana3_0432 at 501.337 kb on - strand, within Shewana3_0432 at 501.582 kb on - strand at 501.784 kb on + strand at 501.881 kb on + strand at 501.886 kb on - strand at 504.658 kb on + strand at 504.666 kb on - strand at 504.740 kb on + strand at 504.740 kb on + strand at 505.160 kb on - strand, within Shewana3_0434 at 505.283 kb on - strand at 505.429 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 501,064 - Shewana3_0432 0.26 +0.3 501,124 + Shewana3_0432 0.35 -4.2 501,149 + Shewana3_0432 0.39 -1.0 501,157 - Shewana3_0432 0.40 +0.6 501,337 - Shewana3_0432 0.67 +1.1 501,582 - -0.6 501,784 + -1.0 501,881 + -0.5 501,886 - +0.1 504,658 + +0.7 504,666 - -0.0 504,740 + +0.7 504,740 + -0.1 505,160 - Shewana3_0434 0.81 -0.5 505,283 - +0.3 505,429 - +0.9
Or see this region's nucleotide sequence