Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0356 and ilvM overlap by 4 nucleotides ilvM and Shewana3_0358 are separated by 79 nucleotides Shewana3_0358 and Shewana3_0359 are separated by 1 nucleotides
Shewana3_0356: Shewana3_0356 - acetolactate synthase 2 catalytic subunit (RefSeq), at 392,186 to 393,859
_0356
Shewana3_0357: ilvM - acetolactate synthase 2 regulatory subunit (RefSeq), at 393,856 to 394,116
ilvM
Shewana3_0358: Shewana3_0358 - dihydroxy-acid dehydratase (RefSeq), at 394,196 to 396,055
_0358
Shewana3_0359: Shewana3_0359 - threonine dehydratase (RefSeq), at 396,057 to 397,700
_0359
Position (kb) 394
395
396
397 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 393.298 kb on + strand, within Shewana3_0356 at 393.343 kb on - strand, within Shewana3_0356 at 393.359 kb on + strand, within Shewana3_0356 at 393.391 kb on - strand, within Shewana3_0356 at 393.398 kb on + strand, within Shewana3_0356 at 393.406 kb on - strand, within Shewana3_0356 at 393.406 kb on - strand, within Shewana3_0356 at 393.419 kb on + strand, within Shewana3_0356 at 393.530 kb on - strand, within Shewana3_0356 at 393.672 kb on - strand, within Shewana3_0356 at 393.719 kb on - strand at 393.880 kb on - strand at 393.943 kb on - strand, within ilvM at 394.024 kb on - strand, within ilvM at 394.071 kb on + strand, within ilvM at 394.120 kb on - strand at 394.133 kb on + strand at 394.152 kb on + strand at 394.184 kb on + strand at 394.473 kb on - strand, within Shewana3_0358 at 394.503 kb on + strand, within Shewana3_0358 at 394.643 kb on - strand, within Shewana3_0358 at 394.755 kb on + strand, within Shewana3_0358 at 394.858 kb on + strand, within Shewana3_0358 at 394.916 kb on + strand, within Shewana3_0358 at 394.916 kb on + strand, within Shewana3_0358 at 394.950 kb on + strand, within Shewana3_0358 at 394.985 kb on - strand, within Shewana3_0358 at 395.037 kb on - strand, within Shewana3_0358 at 395.094 kb on + strand, within Shewana3_0358 at 395.128 kb on + strand, within Shewana3_0358 at 395.136 kb on - strand, within Shewana3_0358 at 395.168 kb on + strand, within Shewana3_0358 at 395.215 kb on - strand, within Shewana3_0358 at 395.272 kb on + strand, within Shewana3_0358 at 395.293 kb on + strand, within Shewana3_0358 at 395.301 kb on - strand, within Shewana3_0358 at 395.378 kb on + strand, within Shewana3_0358 at 395.417 kb on + strand, within Shewana3_0358 at 395.424 kb on + strand, within Shewana3_0358 at 395.573 kb on + strand, within Shewana3_0358 at 395.725 kb on - strand, within Shewana3_0358 at 395.730 kb on - strand, within Shewana3_0358 at 395.827 kb on - strand, within Shewana3_0358 at 395.868 kb on + strand, within Shewana3_0358 at 395.874 kb on + strand at 395.882 kb on - strand at 395.900 kb on + strand at 396.089 kb on + strand at 396.089 kb on + strand at 396.097 kb on - strand at 396.186 kb on + strand at 396.293 kb on - strand, within Shewana3_0359 at 396.420 kb on - strand, within Shewana3_0359 at 396.429 kb on - strand, within Shewana3_0359 at 396.522 kb on - strand, within Shewana3_0359 at 396.649 kb on + strand, within Shewana3_0359 at 396.649 kb on + strand, within Shewana3_0359 at 396.864 kb on - strand, within Shewana3_0359
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 393,298 + Shewana3_0356 0.66 -0.4 393,343 - Shewana3_0356 0.69 -2.3 393,359 + Shewana3_0356 0.70 +2.0 393,391 - Shewana3_0356 0.72 -5.2 393,398 + Shewana3_0356 0.72 +0.5 393,406 - Shewana3_0356 0.73 -3.4 393,406 - Shewana3_0356 0.73 -6.2 393,419 + Shewana3_0356 0.74 +1.9 393,530 - Shewana3_0356 0.80 -5.2 393,672 - Shewana3_0356 0.89 -5.1 393,719 - -4.5 393,880 - -3.8 393,943 - ilvM Shewana3_0357 0.33 -4.6 394,024 - ilvM Shewana3_0357 0.64 -5.6 394,071 + ilvM Shewana3_0357 0.82 +1.6 394,120 - -5.2 394,133 + -2.2 394,152 + +1.1 394,184 + -0.1 394,473 - Shewana3_0358 0.15 -6.8 394,503 + Shewana3_0358 0.17 -4.6 394,643 - Shewana3_0358 0.24 -4.5 394,755 + Shewana3_0358 0.30 -5.1 394,858 + Shewana3_0358 0.36 -3.2 394,916 + Shewana3_0358 0.39 -2.9 394,916 + Shewana3_0358 0.39 -2.6 394,950 + Shewana3_0358 0.41 -6.0 394,985 - Shewana3_0358 0.42 -6.0 395,037 - Shewana3_0358 0.45 -3.7 395,094 + Shewana3_0358 0.48 -5.9 395,128 + Shewana3_0358 0.50 -5.2 395,136 - Shewana3_0358 0.51 -4.5 395,168 + Shewana3_0358 0.52 -3.1 395,215 - Shewana3_0358 0.55 -4.6 395,272 + Shewana3_0358 0.58 -4.9 395,293 + Shewana3_0358 0.59 -6.7 395,301 - Shewana3_0358 0.59 -5.0 395,378 + Shewana3_0358 0.64 -5.3 395,417 + Shewana3_0358 0.66 -5.3 395,424 + Shewana3_0358 0.66 -4.7 395,573 + Shewana3_0358 0.74 -6.0 395,725 - Shewana3_0358 0.82 -1.9 395,730 - Shewana3_0358 0.82 -5.3 395,827 - Shewana3_0358 0.88 -5.0 395,868 + Shewana3_0358 0.90 -3.3 395,874 + -1.8 395,882 - -5.0 395,900 + -4.6 396,089 + -4.8 396,089 + -4.7 396,097 - -4.7 396,186 + -2.7 396,293 - Shewana3_0359 0.14 -4.8 396,420 - Shewana3_0359 0.22 -4.3 396,429 - Shewana3_0359 0.23 -2.5 396,522 - Shewana3_0359 0.28 -2.7 396,649 + Shewana3_0359 0.36 -5.5 396,649 + Shewana3_0359 0.36 -5.1 396,864 - Shewana3_0359 0.49 -4.7
Or see this region's nucleotide sequence