Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0113 and Shewana3_0114 are separated by 2 nucleotides Shewana3_0114 and Shewana3_0115 are separated by 124 nucleotides Shewana3_0115 and Shewana3_0116 are separated by 197 nucleotides
Shewana3_0113: Shewana3_0113 - hypothetical protein (RefSeq), at 129,604 to 130,224
_0113
Shewana3_0114: Shewana3_0114 - hypothetical protein (RefSeq), at 130,227 to 131,216
_0114
Shewana3_0115: Shewana3_0115 - hypothetical protein (RefSeq), at 131,341 to 131,982
_0115
Shewana3_0116: Shewana3_0116 - hypothetical protein (RefSeq), at 132,180 to 132,563
_0116
Position (kb) 130
131
132 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 129.235 kb on + strand at 129.343 kb on + strand at 129.420 kb on - strand at 129.420 kb on - strand at 129.512 kb on + strand at 129.534 kb on + strand at 129.539 kb on + strand at 129.580 kb on + strand at 129.663 kb on + strand at 129.671 kb on - strand, within Shewana3_0113 at 129.816 kb on + strand, within Shewana3_0113 at 129.957 kb on + strand, within Shewana3_0113 at 129.965 kb on - strand, within Shewana3_0113 at 130.117 kb on + strand, within Shewana3_0113 at 130.238 kb on + strand at 130.481 kb on + strand, within Shewana3_0114 at 130.691 kb on - strand, within Shewana3_0114 at 130.698 kb on - strand, within Shewana3_0114 at 130.699 kb on + strand, within Shewana3_0114 at 130.699 kb on + strand, within Shewana3_0114 at 130.699 kb on + strand, within Shewana3_0114 at 130.699 kb on + strand, within Shewana3_0114 at 130.699 kb on + strand, within Shewana3_0114 at 130.934 kb on + strand, within Shewana3_0114 at 130.993 kb on + strand, within Shewana3_0114 at 130.993 kb on + strand, within Shewana3_0114 at 131.037 kb on + strand, within Shewana3_0114 at 131.181 kb on + strand at 131.183 kb on + strand at 131.183 kb on + strand at 131.191 kb on - strand at 131.346 kb on - strand at 131.636 kb on - strand, within Shewana3_0115 at 131.870 kb on + strand, within Shewana3_0115 at 131.878 kb on - strand, within Shewana3_0115 at 131.889 kb on + strand, within Shewana3_0115 at 131.897 kb on - strand, within Shewana3_0115 at 131.903 kb on - strand, within Shewana3_0115 at 131.951 kb on - strand at 132.100 kb on + strand at 132.163 kb on + strand at 132.176 kb on + strand at 132.183 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 129,235 + -0.4 129,343 + +0.1 129,420 - -1.4 129,420 - -1.4 129,512 + +0.5 129,534 + -2.3 129,539 + +3.5 129,580 + +0.6 129,663 + -0.7 129,671 - Shewana3_0113 0.11 -1.1 129,816 + Shewana3_0113 0.34 -1.3 129,957 + Shewana3_0113 0.57 +0.9 129,965 - Shewana3_0113 0.58 -0.9 130,117 + Shewana3_0113 0.83 +2.1 130,238 + -0.9 130,481 + Shewana3_0114 0.26 -1.2 130,691 - Shewana3_0114 0.47 +0.2 130,698 - Shewana3_0114 0.48 -1.2 130,699 + Shewana3_0114 0.48 +1.0 130,699 + Shewana3_0114 0.48 -0.0 130,699 + Shewana3_0114 0.48 +0.3 130,699 + Shewana3_0114 0.48 +0.3 130,699 + Shewana3_0114 0.48 -2.7 130,934 + Shewana3_0114 0.71 -0.9 130,993 + Shewana3_0114 0.77 -0.1 130,993 + Shewana3_0114 0.77 +0.5 131,037 + Shewana3_0114 0.82 +0.6 131,181 + -1.1 131,183 + -0.9 131,183 + -0.7 131,191 - +0.0 131,346 - +0.1 131,636 - Shewana3_0115 0.46 -0.8 131,870 + Shewana3_0115 0.82 +0.2 131,878 - Shewana3_0115 0.84 -2.3 131,889 + Shewana3_0115 0.85 +0.5 131,897 - Shewana3_0115 0.87 +0.1 131,903 - Shewana3_0115 0.88 +0.4 131,951 - -0.9 132,100 + -0.1 132,163 + -0.9 132,176 + +0.0 132,183 - -4.1
Or see this region's nucleotide sequence