Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2061 and Shew_2062 are separated by 184 nucleotides Shew_2062 and Shew_2063 overlap by 7 nucleotides Shew_2063 and Shew_2064 are separated by 126 nucleotides
Shew_2061: Shew_2061 - radical SAM domain-containing protein (RefSeq), at 2,380,599 to 2,381,267
_2061
Shew_2062: Shew_2062 - exsB protein (RefSeq), at 2,381,452 to 2,382,135
_2062
Shew_2063: Shew_2063 - rhomboid family protein (RefSeq), at 2,382,129 to 2,382,713
_2063
Shew_2064: Shew_2064 - excinuclease ABC subunit B (RefSeq), at 2,382,840 to 2,384,858
_2064
Position (kb) 2382
2383 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 2381.331 kb on - strand at 2381.663 kb on + strand, within Shew_2062 at 2381.730 kb on + strand, within Shew_2062 at 2381.978 kb on + strand, within Shew_2062 at 2382.216 kb on - strand, within Shew_2063 at 2382.559 kb on + strand, within Shew_2063 at 2383.002 kb on - strand at 2383.212 kb on + strand, within Shew_2064 at 2383.341 kb on - strand, within Shew_2064
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,381,331 - +0.5 2,381,663 + Shew_2062 0.31 -1.2 2,381,730 + Shew_2062 0.41 -1.0 2,381,978 + Shew_2062 0.77 -3.6 2,382,216 - Shew_2063 0.15 +0.1 2,382,559 + Shew_2063 0.74 -1.4 2,383,002 - +0.4 2,383,212 + Shew_2064 0.18 +0.6 2,383,341 - Shew_2064 0.25 -0.3
Or see this region's nucleotide sequence