Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1524 and Shew_1525 overlap by 1 nucleotides Shew_1525 and Shew_1526 are separated by 3 nucleotides Shew_1526 and Shew_1527 are separated by 9 nucleotides
Shew_1524: Shew_1524 - MotA/TolQ/ExbB proton channel (RefSeq), at 1,748,009 to 1,748,698
_1524
Shew_1525: Shew_1525 - biopolymer transport protein ExbD/TolR (RefSeq), at 1,748,698 to 1,749,132
_1525
Shew_1526: Shew_1526 - TolA family protein (RefSeq), at 1,749,136 to 1,750,071
_1526
Shew_1527: Shew_1527 - TolB domain-containing protein (RefSeq), at 1,750,081 to 1,751,406
_1527
Position (kb) 1749
1750
1751 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1748.296 kb on + strand, within Shew_1524 at 1748.437 kb on + strand, within Shew_1524 at 1748.437 kb on + strand, within Shew_1524 at 1748.950 kb on + strand, within Shew_1525 at 1749.245 kb on - strand, within Shew_1526 at 1749.551 kb on - strand, within Shew_1526 at 1749.810 kb on - strand, within Shew_1526 at 1749.815 kb on - strand, within Shew_1526
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,748,296 + Shew_1524 0.42 -1.6 1,748,437 + Shew_1524 0.62 -2.1 1,748,437 + Shew_1524 0.62 -1.1 1,748,950 + Shew_1525 0.58 +1.6 1,749,245 - Shew_1526 0.12 +0.3 1,749,551 - Shew_1526 0.44 -1.3 1,749,810 - Shew_1526 0.72 -0.7 1,749,815 - Shew_1526 0.73 -3.1
Or see this region's nucleotide sequence