Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1371 and fliL are separated by 99 nucleotides fliL and fliM are separated by 42 nucleotides fliM and fliN are separated by 25 nucleotides fliN and Shew_1375 are separated by 9 nucleotides Shew_1375 and fliP overlap by 4 nucleotides
Shew_1371: Shew_1371 - flagellar hook-length control protein (RefSeq), at 1,580,960 to 1,582,666
_1371
Shew_1372: fliL - flagellar basal body-associated protein FliL (RefSeq), at 1,582,766 to 1,583,287
fliL
Shew_1373: fliM - flagellar motor switch protein FliM (RefSeq), at 1,583,330 to 1,584,355
fliM
Shew_1374: fliN - flagellar motor switch protein (RefSeq), at 1,584,381 to 1,584,764
fliN
Shew_1375: Shew_1375 - flagellar biosynthesis protein, FliO (RefSeq), at 1,584,774 to 1,585,151
_1375
Shew_1376: fliP - flagellar biosynthesis protein FliP (RefSeq), at 1,585,148 to 1,585,915
fliP
Position (kb) 1583
1584
1585 Strain fitness (log2 ratio) -1
0
1 at 1583.140 kb on - strand, within fliL at 1583.258 kb on + strand at 1583.368 kb on + strand at 1583.376 kb on - strand at 1583.828 kb on + strand, within fliM at 1584.333 kb on - strand at 1584.397 kb on - strand at 1584.547 kb on + strand, within fliN at 1584.547 kb on + strand, within fliN at 1584.604 kb on + strand, within fliN at 1584.854 kb on - strand, within Shew_1375 at 1584.959 kb on + strand, within Shew_1375
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,583,140 - fliL Shew_1372 0.72 +0.0 1,583,258 + +0.3 1,583,368 + -0.2 1,583,376 - +0.6 1,583,828 + fliM Shew_1373 0.49 -0.6 1,584,333 - -0.4 1,584,397 - -0.0 1,584,547 + fliN Shew_1374 0.43 -0.8 1,584,547 + fliN Shew_1374 0.43 -1.2 1,584,604 + fliN Shew_1374 0.58 -0.3 1,584,854 - Shew_1375 0.21 -0.9 1,584,959 + Shew_1375 0.49 -0.2
Or see this region's nucleotide sequence