Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0048 and Shew_0049 are separated by 380 nucleotides Shew_0049 and Shew_0050 are separated by 103 nucleotides Shew_0050 and Shew_0051 are separated by 7 nucleotides
Shew_0048: Shew_0048 - short chain dehydrogenase (RefSeq), at 58,313 to 59,020
_0048
Shew_0049: Shew_0049 - choline/carnitine/betaine transporter (RefSeq), at 59,401 to 60,972
_0049
Shew_0050: Shew_0050 - DNA polymerase III subunit epsilon (RefSeq), at 61,076 to 61,795
_0050
Shew_0051: Shew_0051 - cyclic nucleotide-binding protein (RefSeq), at 61,803 to 63,692
_0051
Position (kb)
59
60
61 Strain fitness (log2 ratio)
-2
-1
0
1 at 58.445 kb on - strand, within Shew_0048 at 58.649 kb on - strand, within Shew_0048 at 58.759 kb on + strand, within Shew_0048 at 58.834 kb on - strand, within Shew_0048 at 58.897 kb on + strand, within Shew_0048 at 58.902 kb on + strand, within Shew_0048 at 58.969 kb on + strand at 59.003 kb on - strand at 59.464 kb on + strand at 59.565 kb on + strand, within Shew_0049 at 59.781 kb on - strand, within Shew_0049 at 59.903 kb on - strand, within Shew_0049 at 59.918 kb on - strand, within Shew_0049 at 59.918 kb on - strand, within Shew_0049 at 60.152 kb on + strand, within Shew_0049 at 60.152 kb on + strand, within Shew_0049 at 60.160 kb on - strand, within Shew_0049 at 60.238 kb on - strand, within Shew_0049 at 60.238 kb on - strand, within Shew_0049 at 60.586 kb on + strand, within Shew_0049 at 60.950 kb on - strand at 60.950 kb on - strand at 61.064 kb on - strand at 61.187 kb on + strand, within Shew_0050 at 61.198 kb on - strand, within Shew_0050 at 61.204 kb on - strand, within Shew_0050 at 61.308 kb on + strand, within Shew_0050 at 61.473 kb on - strand, within Shew_0050 at 61.513 kb on + strand, within Shew_0050 at 61.572 kb on + strand, within Shew_0050 at 61.599 kb on + strand, within Shew_0050 at 61.616 kb on + strand, within Shew_0050 at 61.616 kb on + strand, within Shew_0050 at 61.736 kb on - strand at 61.812 kb on - strand at 61.921 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 58,445 - Shew_0048 0.19 -0.0 58,649 - Shew_0048 0.47 +0.3 58,759 + Shew_0048 0.63 -0.4 58,834 - Shew_0048 0.74 +0.5 58,897 + Shew_0048 0.82 +0.2 58,902 + Shew_0048 0.83 -0.6 58,969 + -0.1 59,003 - -0.3 59,464 + +0.7 59,565 + Shew_0049 0.10 -0.7 59,781 - Shew_0049 0.24 -0.4 59,903 - Shew_0049 0.32 -0.0 59,918 - Shew_0049 0.33 -0.5 59,918 - Shew_0049 0.33 -0.7 60,152 + Shew_0049 0.48 +0.5 60,152 + Shew_0049 0.48 -0.1 60,160 - Shew_0049 0.48 +0.0 60,238 - Shew_0049 0.53 +0.8 60,238 - Shew_0049 0.53 -0.6 60,586 + Shew_0049 0.75 -0.1 60,950 - +0.5 60,950 - +0.6 61,064 - +0.2 61,187 + Shew_0050 0.15 -0.4 61,198 - Shew_0050 0.17 -0.4 61,204 - Shew_0050 0.18 +0.2 61,308 + Shew_0050 0.32 -1.8 61,473 - Shew_0050 0.55 -0.1 61,513 + Shew_0050 0.61 +0.7 61,572 + Shew_0050 0.69 +0.2 61,599 + Shew_0050 0.73 -0.7 61,616 + Shew_0050 0.75 +0.0 61,616 + Shew_0050 0.75 +0.0 61,736 - -0.5 61,812 - +0.0 61,921 + +1.1
Or see this region's nucleotide sequence