Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt apt and Synpcc7942_2455 are separated by 161 nucleotides Synpcc7942_2455 and Synpcc7942_2456 are separated by 6 nucleotides Synpcc7942_2456 and glyQ are separated by 114 nucleotides
Synpcc7942_2454: apt - adenine phosphoribosyltransferase, at 2,532,100 to 2,533,374
apt
Synpcc7942_2455: Synpcc7942_2455 - hypothetical protein, at 2,533,536 to 2,534,168
_2455
Synpcc7942_2456: Synpcc7942_2456 - hypothetical protein, at 2,534,175 to 2,535,125
_2456
Synpcc7942_2457: glyQ - glycyl-tRNA synthetase subunit alpha, at 2,535,240 to 2,536,118
glyQ
Position (kb)
2534
2535
2536 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2533.183 kb on - strand, within apt at 2533.185 kb on + strand, within apt at 2533.188 kb on - strand, within apt at 2533.195 kb on - strand, within apt at 2533.199 kb on - strand, within apt at 2533.238 kb on + strand, within apt at 2533.238 kb on - strand, within apt at 2533.280 kb on + strand at 2533.280 kb on + strand at 2533.282 kb on - strand at 2533.291 kb on + strand at 2533.299 kb on - strand at 2533.301 kb on + strand at 2533.301 kb on + strand at 2533.301 kb on + strand at 2533.304 kb on + strand at 2533.307 kb on - strand at 2533.373 kb on + strand at 2533.408 kb on - strand at 2533.408 kb on - strand at 2533.408 kb on - strand at 2533.433 kb on + strand at 2533.565 kb on - strand at 2533.582 kb on - strand at 2534.150 kb on + strand at 2534.738 kb on + strand, within Synpcc7942_2456 at 2534.738 kb on + strand, within Synpcc7942_2456 at 2534.738 kb on + strand, within Synpcc7942_2456 at 2534.738 kb on + strand, within Synpcc7942_2456 at 2534.738 kb on + strand, within Synpcc7942_2456 at 2534.738 kb on + strand, within Synpcc7942_2456 at 2534.746 kb on - strand, within Synpcc7942_2456 at 2534.746 kb on - strand, within Synpcc7942_2456 at 2534.756 kb on - strand, within Synpcc7942_2456 at 2534.758 kb on + strand, within Synpcc7942_2456 at 2534.758 kb on + strand, within Synpcc7942_2456 at 2534.793 kb on + strand, within Synpcc7942_2456 at 2534.839 kb on + strand, within Synpcc7942_2456 at 2534.847 kb on - strand, within Synpcc7942_2456 at 2534.852 kb on + strand, within Synpcc7942_2456 at 2534.854 kb on + strand, within Synpcc7942_2456 at 2534.857 kb on - strand, within Synpcc7942_2456 at 2534.882 kb on + strand, within Synpcc7942_2456 at 2534.882 kb on - strand, within Synpcc7942_2456 at 2534.921 kb on - strand, within Synpcc7942_2456 at 2534.921 kb on - strand, within Synpcc7942_2456 at 2534.943 kb on + strand, within Synpcc7942_2456 at 2535.009 kb on + strand, within Synpcc7942_2456 at 2535.011 kb on + strand, within Synpcc7942_2456 at 2535.017 kb on - strand, within Synpcc7942_2456 at 2535.019 kb on - strand, within Synpcc7942_2456 at 2535.019 kb on - strand, within Synpcc7942_2456 at 2535.030 kb on + strand, within Synpcc7942_2456 at 2535.030 kb on + strand, within Synpcc7942_2456 at 2535.036 kb on + strand at 2535.049 kb on - strand at 2535.050 kb on - strand at 2535.051 kb on - strand at 2535.063 kb on + strand at 2535.084 kb on + strand at 2535.084 kb on + strand at 2535.097 kb on - strand at 2535.168 kb on - strand at 2535.199 kb on - strand at 2536.113 kb on + strand at 2536.113 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 2,533,183 - apt Synpcc7942_2454 0.85 -0.5 2,533,185 + apt Synpcc7942_2454 0.85 -2.7 2,533,188 - apt Synpcc7942_2454 0.85 -2.5 2,533,195 - apt Synpcc7942_2454 0.86 +4.6 2,533,199 - apt Synpcc7942_2454 0.86 -1.5 2,533,238 + apt Synpcc7942_2454 0.89 +0.2 2,533,238 - apt Synpcc7942_2454 0.89 +2.0 2,533,280 + -3.9 2,533,280 + +1.2 2,533,282 - -1.0 2,533,291 + -4.1 2,533,299 - -1.4 2,533,301 + -2.1 2,533,301 + +0.3 2,533,301 + -1.8 2,533,304 + -1.9 2,533,307 - -3.3 2,533,373 + -0.4 2,533,408 - +3.3 2,533,408 - +1.8 2,533,408 - -1.1 2,533,433 + -0.4 2,533,565 - -0.5 2,533,582 - -2.0 2,534,150 + -2.5 2,534,738 + Synpcc7942_2456 0.59 -2.8 2,534,738 + Synpcc7942_2456 0.59 -2.2 2,534,738 + Synpcc7942_2456 0.59 -4.4 2,534,738 + Synpcc7942_2456 0.59 -3.5 2,534,738 + Synpcc7942_2456 0.59 -3.2 2,534,738 + Synpcc7942_2456 0.59 -1.8 2,534,746 - Synpcc7942_2456 0.60 -2.8 2,534,746 - Synpcc7942_2456 0.60 -3.3 2,534,756 - Synpcc7942_2456 0.61 -1.2 2,534,758 + Synpcc7942_2456 0.61 +0.7 2,534,758 + Synpcc7942_2456 0.61 -1.4 2,534,793 + Synpcc7942_2456 0.65 -1.2 2,534,839 + Synpcc7942_2456 0.70 -1.5 2,534,847 - Synpcc7942_2456 0.71 -0.8 2,534,852 + Synpcc7942_2456 0.71 -2.9 2,534,854 + Synpcc7942_2456 0.71 -2.2 2,534,857 - Synpcc7942_2456 0.72 -0.8 2,534,882 + Synpcc7942_2456 0.74 -1.0 2,534,882 - Synpcc7942_2456 0.74 -2.4 2,534,921 - Synpcc7942_2456 0.78 -1.9 2,534,921 - Synpcc7942_2456 0.78 -0.3 2,534,943 + Synpcc7942_2456 0.81 -0.1 2,535,009 + Synpcc7942_2456 0.88 -1.0 2,535,011 + Synpcc7942_2456 0.88 -1.0 2,535,017 - Synpcc7942_2456 0.89 -1.7 2,535,019 - Synpcc7942_2456 0.89 -2.5 2,535,019 - Synpcc7942_2456 0.89 -2.8 2,535,030 + Synpcc7942_2456 0.90 -1.1 2,535,030 + Synpcc7942_2456 0.90 +0.7 2,535,036 + -1.3 2,535,049 - -2.2 2,535,050 - -1.4 2,535,051 - -2.3 2,535,063 + -3.9 2,535,084 + -1.9 2,535,084 + -1.1 2,535,097 - -2.8 2,535,168 - +2.4 2,535,199 - +1.3 2,536,113 + -1.8 2,536,113 + -0.9
Or see this region's nucleotide sequence