Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisB and Synpcc7942_2347 overlap by 4 nucleotides Synpcc7942_2347 and queA are separated by 18 nucleotides
Synpcc7942_2346: hisB - HAD-superfamily subfamily IA, at 2,413,356 to 2,414,141
hisB
Synpcc7942_2347: Synpcc7942_2347 - hypothetical protein, at 2,414,138 to 2,415,214
_2347
Synpcc7942_2348: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 2,415,233 to 2,416,312
queA
Position (kb)
2414
2415
2416 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2413.217 kb on + strand at 2413.291 kb on - strand at 2413.305 kb on + strand at 2413.327 kb on + strand at 2413.327 kb on + strand at 2413.327 kb on + strand at 2413.327 kb on + strand at 2413.327 kb on + strand at 2413.335 kb on - strand at 2413.335 kb on - strand at 2413.335 kb on - strand at 2413.335 kb on - strand at 2413.335 kb on - strand at 2413.335 kb on - strand at 2413.338 kb on - strand at 2413.340 kb on + strand at 2413.341 kb on + strand at 2413.351 kb on + strand at 2413.354 kb on + strand at 2413.356 kb on - strand at 2413.359 kb on - strand at 2413.359 kb on - strand at 2413.359 kb on - strand at 2413.364 kb on + strand at 2413.364 kb on + strand at 2413.364 kb on + strand at 2414.120 kb on + strand at 2414.120 kb on + strand at 2414.138 kb on - strand at 2414.138 kb on - strand at 2414.138 kb on - strand at 2414.151 kb on + strand at 2414.193 kb on - strand at 2414.202 kb on + strand at 2414.251 kb on - strand, within Synpcc7942_2347 at 2414.251 kb on - strand, within Synpcc7942_2347 at 2414.251 kb on - strand, within Synpcc7942_2347 at 2414.251 kb on - strand, within Synpcc7942_2347 at 2414.266 kb on + strand, within Synpcc7942_2347 at 2414.266 kb on + strand, within Synpcc7942_2347 at 2414.301 kb on + strand, within Synpcc7942_2347 at 2414.301 kb on + strand at 2414.309 kb on - strand, within Synpcc7942_2347 at 2414.336 kb on + strand, within Synpcc7942_2347 at 2414.361 kb on + strand, within Synpcc7942_2347 at 2414.361 kb on + strand at 2414.365 kb on + strand, within Synpcc7942_2347 at 2414.365 kb on + strand, within Synpcc7942_2347 at 2414.369 kb on - strand, within Synpcc7942_2347 at 2414.462 kb on + strand at 2414.522 kb on + strand, within Synpcc7942_2347 at 2414.530 kb on - strand, within Synpcc7942_2347 at 2414.552 kb on + strand, within Synpcc7942_2347 at 2414.616 kb on + strand, within Synpcc7942_2347 at 2414.644 kb on + strand at 2414.679 kb on + strand, within Synpcc7942_2347 at 2414.679 kb on + strand, within Synpcc7942_2347 at 2414.679 kb on + strand, within Synpcc7942_2347 at 2414.681 kb on + strand, within Synpcc7942_2347 at 2414.681 kb on + strand, within Synpcc7942_2347 at 2414.699 kb on - strand, within Synpcc7942_2347 at 2414.734 kb on + strand, within Synpcc7942_2347 at 2414.737 kb on + strand, within Synpcc7942_2347 at 2414.742 kb on - strand, within Synpcc7942_2347 at 2414.742 kb on - strand, within Synpcc7942_2347 at 2414.788 kb on + strand, within Synpcc7942_2347 at 2414.788 kb on + strand, within Synpcc7942_2347 at 2414.806 kb on + strand, within Synpcc7942_2347 at 2414.806 kb on + strand, within Synpcc7942_2347 at 2414.821 kb on - strand, within Synpcc7942_2347 at 2414.837 kb on + strand, within Synpcc7942_2347 at 2414.861 kb on + strand, within Synpcc7942_2347 at 2414.895 kb on + strand, within Synpcc7942_2347 at 2414.912 kb on + strand at 2414.912 kb on + strand, within Synpcc7942_2347 at 2414.912 kb on + strand, within Synpcc7942_2347 at 2414.920 kb on - strand, within Synpcc7942_2347 at 2414.920 kb on - strand, within Synpcc7942_2347 at 2414.920 kb on - strand, within Synpcc7942_2347 at 2414.920 kb on - strand, within Synpcc7942_2347 at 2414.958 kb on - strand, within Synpcc7942_2347 at 2414.963 kb on + strand, within Synpcc7942_2347 at 2414.976 kb on + strand, within Synpcc7942_2347 at 2414.976 kb on - strand, within Synpcc7942_2347 at 2415.003 kb on + strand, within Synpcc7942_2347 at 2415.011 kb on - strand, within Synpcc7942_2347 at 2415.016 kb on - strand, within Synpcc7942_2347 at 2415.039 kb on - strand, within Synpcc7942_2347 at 2415.050 kb on - strand, within Synpcc7942_2347 at 2415.050 kb on - strand, within Synpcc7942_2347 at 2415.055 kb on - strand, within Synpcc7942_2347 at 2415.085 kb on - strand, within Synpcc7942_2347 at 2415.089 kb on + strand, within Synpcc7942_2347 at 2415.097 kb on - strand, within Synpcc7942_2347 at 2415.097 kb on - strand, within Synpcc7942_2347 at 2415.097 kb on - strand, within Synpcc7942_2347 at 2415.123 kb on + strand at 2415.123 kb on + strand at 2415.131 kb on - strand at 2415.140 kb on + strand at 2415.202 kb on + strand at 2415.210 kb on - strand at 2415.210 kb on - strand at 2415.210 kb on - strand at 2415.210 kb on - strand at 2415.216 kb on + strand at 2415.240 kb on + strand at 2415.248 kb on - strand at 2415.248 kb on - strand at 2415.288 kb on + strand at 2415.302 kb on + strand at 2415.302 kb on + strand at 2415.302 kb on + strand at 2415.302 kb on + strand at 2415.309 kb on + strand at 2415.310 kb on - strand at 2415.310 kb on - strand at 2415.317 kb on - strand at 2415.344 kb on + strand, within queA at 2415.439 kb on + strand, within queA at 2415.447 kb on - strand, within queA at 2415.447 kb on - strand, within queA at 2415.447 kb on - strand, within queA at 2415.447 kb on - strand, within queA at 2415.455 kb on - strand, within queA at 2415.487 kb on + strand, within queA at 2415.531 kb on - strand, within queA at 2415.564 kb on - strand, within queA at 2415.579 kb on + strand, within queA at 2415.579 kb on + strand, within queA at 2415.579 kb on + strand, within queA at 2415.587 kb on - strand, within queA at 2415.648 kb on + strand, within queA at 2415.682 kb on + strand, within queA at 2415.694 kb on + strand, within queA at 2415.703 kb on + strand, within queA at 2415.725 kb on + strand, within queA at 2415.731 kb on + strand, within queA at 2415.731 kb on + strand, within queA at 2415.734 kb on - strand, within queA at 2415.750 kb on + strand, within queA at 2415.750 kb on + strand, within queA at 2415.770 kb on + strand, within queA at 2415.770 kb on + strand, within queA at 2415.770 kb on + strand, within queA at 2415.778 kb on - strand, within queA at 2415.778 kb on - strand, within queA at 2415.797 kb on - strand, within queA at 2415.798 kb on - strand, within queA at 2415.812 kb on + strand, within queA at 2415.815 kb on - strand, within queA at 2415.825 kb on + strand, within queA at 2415.828 kb on - strand, within queA at 2415.842 kb on + strand, within queA at 2415.843 kb on + strand, within queA at 2415.849 kb on - strand, within queA at 2415.861 kb on - strand, within queA at 2415.869 kb on + strand, within queA at 2415.870 kb on - strand, within queA at 2415.881 kb on - strand, within queA at 2415.891 kb on + strand, within queA at 2415.894 kb on - strand, within queA at 2415.900 kb on - strand, within queA at 2415.909 kb on - strand, within queA at 2415.923 kb on - strand, within queA at 2415.923 kb on - strand, within queA at 2415.924 kb on + strand, within queA at 2415.927 kb on + strand, within queA at 2415.934 kb on + strand, within queA at 2416.027 kb on + strand, within queA at 2416.036 kb on - strand, within queA at 2416.056 kb on - strand, within queA at 2416.064 kb on + strand, within queA at 2416.064 kb on + strand, within queA at 2416.072 kb on - strand, within queA at 2416.075 kb on + strand, within queA at 2416.075 kb on + strand, within queA at 2416.075 kb on + strand, within queA at 2416.118 kb on + strand, within queA at 2416.122 kb on + strand, within queA at 2416.122 kb on + strand, within queA at 2416.192 kb on - strand, within queA at 2416.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 2,413,217 + -0.5 2,413,291 - -1.1 2,413,305 + +1.7 2,413,327 + -1.2 2,413,327 + -2.5 2,413,327 + +0.4 2,413,327 + +0.3 2,413,327 + -2.0 2,413,335 - +1.5 2,413,335 - +1.0 2,413,335 - +0.1 2,413,335 - +1.6 2,413,335 - -0.9 2,413,335 - -2.2 2,413,338 - +2.4 2,413,340 + +0.6 2,413,341 + +1.3 2,413,351 + +0.1 2,413,354 + +2.2 2,413,356 - -0.5 2,413,359 - -1.0 2,413,359 - +0.6 2,413,359 - +1.6 2,413,364 + +2.6 2,413,364 + -2.6 2,413,364 + +3.7 2,414,120 + -1.7 2,414,120 + +1.6 2,414,138 - -0.1 2,414,138 - -1.1 2,414,138 - -1.2 2,414,151 + -1.5 2,414,193 - -1.0 2,414,202 + +0.5 2,414,251 - Synpcc7942_2347 0.10 -0.1 2,414,251 - Synpcc7942_2347 0.10 -1.8 2,414,251 - Synpcc7942_2347 0.10 -0.5 2,414,251 - Synpcc7942_2347 0.10 -2.5 2,414,266 + Synpcc7942_2347 0.12 -2.5 2,414,266 + Synpcc7942_2347 0.12 -1.7 2,414,301 + Synpcc7942_2347 0.15 +2.2 2,414,301 + -0.5 2,414,309 - Synpcc7942_2347 0.16 -4.8 2,414,336 + Synpcc7942_2347 0.18 -2.1 2,414,361 + Synpcc7942_2347 0.21 -2.6 2,414,361 + +1.9 2,414,365 + Synpcc7942_2347 0.21 -3.0 2,414,365 + Synpcc7942_2347 0.21 -2.5 2,414,369 - Synpcc7942_2347 0.21 -0.4 2,414,462 + +0.9 2,414,522 + Synpcc7942_2347 0.36 -1.4 2,414,530 - Synpcc7942_2347 0.36 -1.6 2,414,552 + Synpcc7942_2347 0.38 -0.2 2,414,616 + Synpcc7942_2347 0.44 -0.8 2,414,644 + -0.7 2,414,679 + Synpcc7942_2347 0.50 -0.9 2,414,679 + Synpcc7942_2347 0.50 -3.1 2,414,679 + Synpcc7942_2347 0.50 -0.1 2,414,681 + Synpcc7942_2347 0.50 -1.7 2,414,681 + Synpcc7942_2347 0.50 -1.5 2,414,699 - Synpcc7942_2347 0.52 -2.1 2,414,734 + Synpcc7942_2347 0.55 -0.7 2,414,737 + Synpcc7942_2347 0.56 -2.1 2,414,742 - Synpcc7942_2347 0.56 -2.9 2,414,742 - Synpcc7942_2347 0.56 -2.5 2,414,788 + Synpcc7942_2347 0.60 -2.3 2,414,788 + Synpcc7942_2347 0.60 -1.4 2,414,806 + Synpcc7942_2347 0.62 -2.5 2,414,806 + Synpcc7942_2347 0.62 -0.7 2,414,821 - Synpcc7942_2347 0.63 -1.5 2,414,837 + Synpcc7942_2347 0.65 -1.0 2,414,861 + Synpcc7942_2347 0.67 -2.9 2,414,895 + Synpcc7942_2347 0.70 -1.0 2,414,912 + +0.5 2,414,912 + Synpcc7942_2347 0.72 -0.5 2,414,912 + Synpcc7942_2347 0.72 -1.8 2,414,920 - Synpcc7942_2347 0.73 +0.2 2,414,920 - Synpcc7942_2347 0.73 +0.5 2,414,920 - Synpcc7942_2347 0.73 -0.5 2,414,920 - Synpcc7942_2347 0.73 -3.9 2,414,958 - Synpcc7942_2347 0.76 -1.0 2,414,963 + Synpcc7942_2347 0.77 -1.5 2,414,976 + Synpcc7942_2347 0.78 -0.7 2,414,976 - Synpcc7942_2347 0.78 +0.5 2,415,003 + Synpcc7942_2347 0.80 -1.6 2,415,011 - Synpcc7942_2347 0.81 -0.4 2,415,016 - Synpcc7942_2347 0.82 -2.3 2,415,039 - Synpcc7942_2347 0.84 -1.1 2,415,050 - Synpcc7942_2347 0.85 -2.0 2,415,050 - Synpcc7942_2347 0.85 -1.4 2,415,055 - Synpcc7942_2347 0.85 -1.6 2,415,085 - Synpcc7942_2347 0.88 +0.5 2,415,089 + Synpcc7942_2347 0.88 -2.0 2,415,097 - Synpcc7942_2347 0.89 -1.8 2,415,097 - Synpcc7942_2347 0.89 -0.5 2,415,097 - Synpcc7942_2347 0.89 -2.9 2,415,123 + -0.6 2,415,123 + +0.1 2,415,131 - -0.1 2,415,140 + -0.8 2,415,202 + -1.5 2,415,210 - +1.1 2,415,210 - +1.9 2,415,210 - -1.1 2,415,210 - -1.9 2,415,216 + -2.2 2,415,240 + -1.4 2,415,248 - -1.4 2,415,248 - -0.0 2,415,288 + -1.6 2,415,302 + -2.8 2,415,302 + -2.7 2,415,302 + -3.4 2,415,302 + -1.4 2,415,309 + -1.1 2,415,310 - -1.5 2,415,310 - -3.6 2,415,317 - +4.0 2,415,344 + queA Synpcc7942_2348 0.10 +0.1 2,415,439 + queA Synpcc7942_2348 0.19 +1.2 2,415,447 - queA Synpcc7942_2348 0.20 -1.6 2,415,447 - queA Synpcc7942_2348 0.20 -1.9 2,415,447 - queA Synpcc7942_2348 0.20 -2.2 2,415,447 - queA Synpcc7942_2348 0.20 +1.5 2,415,455 - queA Synpcc7942_2348 0.21 -0.2 2,415,487 + queA Synpcc7942_2348 0.24 +0.1 2,415,531 - queA Synpcc7942_2348 0.28 -2.0 2,415,564 - queA Synpcc7942_2348 0.31 -1.0 2,415,579 + queA Synpcc7942_2348 0.32 -2.2 2,415,579 + queA Synpcc7942_2348 0.32 +0.8 2,415,579 + queA Synpcc7942_2348 0.32 -0.7 2,415,587 - queA Synpcc7942_2348 0.33 -3.2 2,415,648 + queA Synpcc7942_2348 0.38 +0.5 2,415,682 + queA Synpcc7942_2348 0.42 -2.0 2,415,694 + queA Synpcc7942_2348 0.43 +0.1 2,415,703 + queA Synpcc7942_2348 0.44 +1.2 2,415,725 + queA Synpcc7942_2348 0.46 -2.3 2,415,731 + queA Synpcc7942_2348 0.46 +1.2 2,415,731 + queA Synpcc7942_2348 0.46 -1.2 2,415,734 - queA Synpcc7942_2348 0.46 -0.4 2,415,750 + queA Synpcc7942_2348 0.48 +0.1 2,415,750 + queA Synpcc7942_2348 0.48 -2.8 2,415,770 + queA Synpcc7942_2348 0.50 -1.9 2,415,770 + queA Synpcc7942_2348 0.50 +1.7 2,415,770 + queA Synpcc7942_2348 0.50 -3.1 2,415,778 - queA Synpcc7942_2348 0.50 -1.0 2,415,778 - queA Synpcc7942_2348 0.50 -0.1 2,415,797 - queA Synpcc7942_2348 0.52 -2.4 2,415,798 - queA Synpcc7942_2348 0.52 -1.8 2,415,812 + queA Synpcc7942_2348 0.54 -1.0 2,415,815 - queA Synpcc7942_2348 0.54 -0.5 2,415,825 + queA Synpcc7942_2348 0.55 -1.6 2,415,828 - queA Synpcc7942_2348 0.55 -0.0 2,415,842 + queA Synpcc7942_2348 0.56 -1.4 2,415,843 + queA Synpcc7942_2348 0.56 -1.8 2,415,849 - queA Synpcc7942_2348 0.57 -0.8 2,415,861 - queA Synpcc7942_2348 0.58 +0.3 2,415,869 + queA Synpcc7942_2348 0.59 -1.2 2,415,870 - queA Synpcc7942_2348 0.59 -0.4 2,415,881 - queA Synpcc7942_2348 0.60 +0.1 2,415,891 + queA Synpcc7942_2348 0.61 +2.3 2,415,894 - queA Synpcc7942_2348 0.61 +0.3 2,415,900 - queA Synpcc7942_2348 0.62 +0.1 2,415,909 - queA Synpcc7942_2348 0.63 -1.7 2,415,923 - queA Synpcc7942_2348 0.64 -2.0 2,415,923 - queA Synpcc7942_2348 0.64 +1.7 2,415,924 + queA Synpcc7942_2348 0.64 -1.9 2,415,927 + queA Synpcc7942_2348 0.64 -1.5 2,415,934 + queA Synpcc7942_2348 0.65 -2.7 2,416,027 + queA Synpcc7942_2348 0.74 -1.4 2,416,036 - queA Synpcc7942_2348 0.74 -1.2 2,416,056 - queA Synpcc7942_2348 0.76 -1.5 2,416,064 + queA Synpcc7942_2348 0.77 -0.4 2,416,064 + queA Synpcc7942_2348 0.77 -1.5 2,416,072 - queA Synpcc7942_2348 0.78 +0.3 2,416,075 + queA Synpcc7942_2348 0.78 -1.6 2,416,075 + queA Synpcc7942_2348 0.78 -0.1 2,416,075 + queA Synpcc7942_2348 0.78 -0.7 2,416,118 + queA Synpcc7942_2348 0.82 -1.2 2,416,122 + queA Synpcc7942_2348 0.82 -3.2 2,416,122 + queA Synpcc7942_2348 0.82 -0.0 2,416,192 - queA Synpcc7942_2348 0.89 +0.8 2,416,206 + -0.5
Or see this region's nucleotide sequence