Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2119 and Synpcc7942_2120 are separated by 41 nucleotides Synpcc7942_2120 and spoIIA are separated by 2 nucleotides spoIIA and carA are separated by 50 nucleotides
Synpcc7942_2119: Synpcc7942_2119 - RNA methyltransferase TrmH, group 3, at 2,200,097 to 2,201,656
_2119
Synpcc7942_2120: Synpcc7942_2120 - Ribonuclease III, at 2,201,698 to 2,202,114
_2120
Synpcc7942_2121: spoIIA - anti-anti-sigma regulatory factor, SpoIIAA, at 2,202,117 to 2,202,509
spoIIA
Synpcc7942_2122: carA - carbamoyl phosphate synthase small subunit, at 2,202,560 to 2,203,735
carA
Position (kb)
2201
2202
2203 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2201.339 kb on + strand, within Synpcc7942_2119 at 2201.697 kb on - strand at 2201.757 kb on + strand, within Synpcc7942_2120 at 2201.757 kb on + strand, within Synpcc7942_2120 at 2201.765 kb on - strand, within Synpcc7942_2120 at 2201.765 kb on - strand, within Synpcc7942_2120 at 2201.775 kb on - strand, within Synpcc7942_2120 at 2201.778 kb on + strand, within Synpcc7942_2120 at 2201.779 kb on + strand, within Synpcc7942_2120 at 2201.779 kb on + strand, within Synpcc7942_2120 at 2201.787 kb on - strand, within Synpcc7942_2120 at 2201.787 kb on - strand, within Synpcc7942_2120 at 2201.879 kb on + strand, within Synpcc7942_2120 at 2201.889 kb on + strand, within Synpcc7942_2120 at 2201.889 kb on + strand, within Synpcc7942_2120 at 2201.889 kb on + strand, within Synpcc7942_2120 at 2201.889 kb on + strand, within Synpcc7942_2120 at 2201.897 kb on - strand, within Synpcc7942_2120 at 2202.018 kb on + strand, within Synpcc7942_2120 at 2202.026 kb on - strand, within Synpcc7942_2120 at 2202.028 kb on + strand, within Synpcc7942_2120 at 2202.029 kb on + strand, within Synpcc7942_2120 at 2202.060 kb on + strand, within Synpcc7942_2120 at 2202.093 kb on + strand at 2202.093 kb on + strand at 2202.100 kb on - strand at 2202.101 kb on - strand at 2202.546 kb on + strand at 2202.557 kb on + strand at 2202.566 kb on - strand at 2202.566 kb on - strand at 2202.566 kb on - strand at 2202.575 kb on + strand at 2202.590 kb on + strand at 2202.605 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 2,201,339 + Synpcc7942_2119 0.80 -0.3 2,201,697 - -1.9 2,201,757 + Synpcc7942_2120 0.14 -0.7 2,201,757 + Synpcc7942_2120 0.14 -1.9 2,201,765 - Synpcc7942_2120 0.16 -1.2 2,201,765 - Synpcc7942_2120 0.16 +1.9 2,201,775 - Synpcc7942_2120 0.18 -3.2 2,201,778 + Synpcc7942_2120 0.19 -2.7 2,201,779 + Synpcc7942_2120 0.19 +1.5 2,201,779 + Synpcc7942_2120 0.19 -1.9 2,201,787 - Synpcc7942_2120 0.21 +0.5 2,201,787 - Synpcc7942_2120 0.21 -4.0 2,201,879 + Synpcc7942_2120 0.43 -1.7 2,201,889 + Synpcc7942_2120 0.46 -0.7 2,201,889 + Synpcc7942_2120 0.46 -1.4 2,201,889 + Synpcc7942_2120 0.46 -2.5 2,201,889 + Synpcc7942_2120 0.46 -3.0 2,201,897 - Synpcc7942_2120 0.48 -4.4 2,202,018 + Synpcc7942_2120 0.77 -0.7 2,202,026 - Synpcc7942_2120 0.79 +0.4 2,202,028 + Synpcc7942_2120 0.79 -3.1 2,202,029 + Synpcc7942_2120 0.79 -1.3 2,202,060 + Synpcc7942_2120 0.87 -3.2 2,202,093 + -2.8 2,202,093 + +0.1 2,202,100 - -2.9 2,202,101 - +0.7 2,202,546 + +1.3 2,202,557 + +1.0 2,202,566 - -2.0 2,202,566 - -2.4 2,202,566 - -1.3 2,202,575 + -1.9 2,202,590 + -2.2 2,202,605 - -2.1
Or see this region's nucleotide sequence