Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1797 and Synpcc7942_1798 are separated by 487 nucleotides Synpcc7942_1798 and hypE are separated by 449 nucleotides
Synpcc7942_1797: Synpcc7942_1797 - hypothetical protein, at 1,866,873 to 1,867,376
_1797
Synpcc7942_1798: Synpcc7942_1798 - hypothetical protein, at 1,867,864 to 1,868,490
_1798
Synpcc7942_1799: hypE - Hydrogenase expression/formation protein HypE, at 1,868,940 to 1,869,971
hypE
Position (kb) 1867
1868
1869 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 1866.869 kb on + strand at 1866.869 kb on + strand at 1866.870 kb on + strand at 1866.877 kb on - strand at 1866.877 kb on - strand at 1866.877 kb on - strand at 1866.879 kb on + strand at 1866.879 kb on + strand at 1866.879 kb on + strand at 1866.879 kb on + strand at 1866.880 kb on + strand at 1866.880 kb on + strand at 1866.887 kb on - strand at 1866.887 kb on - strand at 1866.887 kb on - strand at 1866.887 kb on - strand at 1866.925 kb on + strand, within Synpcc7942_1797 at 1866.943 kb on + strand, within Synpcc7942_1797 at 1867.006 kb on + strand, within Synpcc7942_1797 at 1867.006 kb on + strand, within Synpcc7942_1797 at 1867.060 kb on - strand, within Synpcc7942_1797 at 1867.078 kb on - strand, within Synpcc7942_1797 at 1867.093 kb on + strand at 1867.101 kb on - strand, within Synpcc7942_1797 at 1867.101 kb on - strand, within Synpcc7942_1797 at 1867.105 kb on + strand, within Synpcc7942_1797 at 1867.105 kb on + strand, within Synpcc7942_1797 at 1867.105 kb on + strand, within Synpcc7942_1797 at 1867.113 kb on - strand, within Synpcc7942_1797 at 1867.154 kb on + strand, within Synpcc7942_1797 at 1867.154 kb on + strand, within Synpcc7942_1797 at 1867.162 kb on - strand, within Synpcc7942_1797 at 1867.162 kb on - strand, within Synpcc7942_1797 at 1867.162 kb on - strand, within Synpcc7942_1797 at 1867.164 kb on + strand, within Synpcc7942_1797 at 1867.172 kb on - strand, within Synpcc7942_1797 at 1867.172 kb on - strand, within Synpcc7942_1797 at 1867.181 kb on + strand, within Synpcc7942_1797 at 1867.194 kb on + strand, within Synpcc7942_1797 at 1867.196 kb on + strand, within Synpcc7942_1797 at 1867.229 kb on + strand, within Synpcc7942_1797 at 1867.229 kb on + strand, within Synpcc7942_1797 at 1867.237 kb on + strand, within Synpcc7942_1797 at 1867.237 kb on + strand, within Synpcc7942_1797 at 1867.239 kb on + strand, within Synpcc7942_1797 at 1867.247 kb on + strand, within Synpcc7942_1797 at 1867.263 kb on + strand, within Synpcc7942_1797 at 1867.271 kb on - strand, within Synpcc7942_1797 at 1867.271 kb on - strand, within Synpcc7942_1797 at 1867.271 kb on - strand, within Synpcc7942_1797 at 1867.271 kb on - strand, within Synpcc7942_1797 at 1867.278 kb on - strand, within Synpcc7942_1797 at 1867.296 kb on + strand, within Synpcc7942_1797 at 1867.298 kb on - strand, within Synpcc7942_1797 at 1867.311 kb on + strand, within Synpcc7942_1797 at 1867.312 kb on + strand, within Synpcc7942_1797 at 1867.321 kb on - strand, within Synpcc7942_1797 at 1867.331 kb on - strand at 1867.337 kb on + strand at 1867.347 kb on + strand at 1867.347 kb on + strand at 1867.347 kb on + strand at 1867.355 kb on - strand at 1867.365 kb on + strand at 1867.373 kb on - strand at 1867.408 kb on + strand at 1867.418 kb on + strand at 1867.426 kb on - strand at 1867.468 kb on + strand at 1867.843 kb on + strand at 1867.871 kb on + strand at 1867.879 kb on - strand at 1867.879 kb on - strand at 1867.879 kb on - strand at 1867.879 kb on - strand at 1867.881 kb on + strand at 1868.025 kb on + strand, within Synpcc7942_1798 at 1868.040 kb on - strand, within Synpcc7942_1798 at 1868.136 kb on + strand, within Synpcc7942_1798 at 1868.157 kb on + strand, within Synpcc7942_1798 at 1868.265 kb on + strand, within Synpcc7942_1798 at 1868.286 kb on + strand, within Synpcc7942_1798 at 1868.318 kb on - strand, within Synpcc7942_1798 at 1868.375 kb on - strand, within Synpcc7942_1798 at 1868.426 kb on + strand at 1868.426 kb on + strand, within Synpcc7942_1798 at 1868.451 kb on + strand at 1868.492 kb on - strand at 1868.521 kb on + strand at 1868.752 kb on - strand at 1868.782 kb on + strand at 1868.789 kb on - strand at 1868.790 kb on - strand at 1868.792 kb on + strand at 1868.823 kb on - strand at 1868.836 kb on + strand at 1868.845 kb on - strand at 1868.876 kb on - strand at 1868.886 kb on + strand at 1868.886 kb on + strand at 1868.894 kb on + strand at 1868.894 kb on - strand at 1868.896 kb on + strand at 1868.896 kb on + strand at 1868.904 kb on - strand at 1868.904 kb on - strand at 1868.904 kb on - strand at 1868.941 kb on - strand at 1868.943 kb on + strand at 1868.943 kb on + strand at 1868.943 kb on + strand at 1868.943 kb on + strand at 1868.943 kb on + strand at 1868.951 kb on - strand at 1868.951 kb on - strand at 1868.951 kb on - strand at 1868.961 kb on + strand at 1868.961 kb on + strand at 1868.961 kb on - strand at 1868.966 kb on + strand at 1868.999 kb on - strand at 1868.999 kb on - strand at 1868.999 kb on - strand at 1869.137 kb on + strand, within hypE at 1869.162 kb on + strand, within hypE at 1869.165 kb on + strand, within hypE at 1869.193 kb on - strand, within hypE at 1869.195 kb on + strand, within hypE at 1869.195 kb on + strand, within hypE at 1869.203 kb on - strand, within hypE at 1869.294 kb on - strand, within hypE at 1869.302 kb on + strand, within hypE at 1869.302 kb on + strand, within hypE at 1869.310 kb on - strand, within hypE at 1869.312 kb on + strand, within hypE at 1869.312 kb on + strand, within hypE at 1869.312 kb on + strand, within hypE at 1869.312 kb on + strand, within hypE at 1869.320 kb on - strand, within hypE at 1869.320 kb on - strand, within hypE at 1869.320 kb on - strand, within hypE at 1869.320 kb on - strand, within hypE at 1869.320 kb on - strand, within hypE at 1869.340 kb on - strand, within hypE at 1869.362 kb on - strand, within hypE at 1869.462 kb on + strand, within hypE at 1869.462 kb on + strand, within hypE at 1869.470 kb on - strand, within hypE at 1869.470 kb on - strand, within hypE at 1869.488 kb on + strand, within hypE
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 1,866,869 + -2.1 1,866,869 + -0.5 1,866,870 + -0.9 1,866,877 - -0.3 1,866,877 - -1.8 1,866,877 - -1.0 1,866,879 + -1.2 1,866,879 + -1.1 1,866,879 + +0.5 1,866,879 + +0.9 1,866,880 + +0.2 1,866,880 + -1.6 1,866,887 - +0.2 1,866,887 - -0.7 1,866,887 - -0.0 1,866,887 - +1.8 1,866,925 + Synpcc7942_1797 0.10 -0.7 1,866,943 + Synpcc7942_1797 0.14 +2.4 1,867,006 + Synpcc7942_1797 0.26 -2.0 1,867,006 + Synpcc7942_1797 0.26 +0.3 1,867,060 - Synpcc7942_1797 0.37 +2.6 1,867,078 - Synpcc7942_1797 0.41 +0.2 1,867,093 + -0.8 1,867,101 - Synpcc7942_1797 0.45 -1.9 1,867,101 - Synpcc7942_1797 0.45 +3.5 1,867,105 + Synpcc7942_1797 0.46 +1.9 1,867,105 + Synpcc7942_1797 0.46 +0.9 1,867,105 + Synpcc7942_1797 0.46 +0.9 1,867,113 - Synpcc7942_1797 0.48 +0.3 1,867,154 + Synpcc7942_1797 0.56 -1.5 1,867,154 + Synpcc7942_1797 0.56 -1.1 1,867,162 - Synpcc7942_1797 0.57 -0.0 1,867,162 - Synpcc7942_1797 0.57 +1.2 1,867,162 - Synpcc7942_1797 0.57 +1.9 1,867,164 + Synpcc7942_1797 0.58 +0.2 1,867,172 - Synpcc7942_1797 0.59 -0.2 1,867,172 - Synpcc7942_1797 0.59 -1.7 1,867,181 + Synpcc7942_1797 0.61 +1.1 1,867,194 + Synpcc7942_1797 0.64 -0.3 1,867,196 + Synpcc7942_1797 0.64 +0.8 1,867,229 + Synpcc7942_1797 0.71 +0.4 1,867,229 + Synpcc7942_1797 0.71 -0.1 1,867,237 + Synpcc7942_1797 0.72 -3.2 1,867,237 + Synpcc7942_1797 0.72 -1.5 1,867,239 + Synpcc7942_1797 0.73 -0.2 1,867,247 + Synpcc7942_1797 0.74 +0.8 1,867,263 + Synpcc7942_1797 0.77 -2.3 1,867,271 - Synpcc7942_1797 0.79 +2.9 1,867,271 - Synpcc7942_1797 0.79 +0.4 1,867,271 - Synpcc7942_1797 0.79 +0.7 1,867,271 - Synpcc7942_1797 0.79 -3.6 1,867,278 - Synpcc7942_1797 0.80 +2.7 1,867,296 + Synpcc7942_1797 0.84 +0.1 1,867,298 - Synpcc7942_1797 0.84 -2.4 1,867,311 + Synpcc7942_1797 0.87 -0.2 1,867,312 + Synpcc7942_1797 0.87 +2.8 1,867,321 - Synpcc7942_1797 0.89 -2.1 1,867,331 - +1.4 1,867,337 + -0.6 1,867,347 + -1.0 1,867,347 + +0.7 1,867,347 + -0.2 1,867,355 - +2.8 1,867,365 + -1.1 1,867,373 - -1.8 1,867,408 + -4.5 1,867,418 + +0.3 1,867,426 - +1.4 1,867,468 + -2.1 1,867,843 + -0.3 1,867,871 + +0.4 1,867,879 - +1.4 1,867,879 - +0.3 1,867,879 - +1.9 1,867,879 - -3.0 1,867,881 + -1.1 1,868,025 + Synpcc7942_1798 0.26 -1.3 1,868,040 - Synpcc7942_1798 0.28 +0.7 1,868,136 + Synpcc7942_1798 0.43 +2.9 1,868,157 + Synpcc7942_1798 0.47 -1.0 1,868,265 + Synpcc7942_1798 0.64 +2.0 1,868,286 + Synpcc7942_1798 0.67 -0.6 1,868,318 - Synpcc7942_1798 0.72 -1.3 1,868,375 - Synpcc7942_1798 0.81 -2.6 1,868,426 + -0.8 1,868,426 + Synpcc7942_1798 0.90 +1.6 1,868,451 + +0.2 1,868,492 - -2.7 1,868,521 + +1.8 1,868,752 - -0.3 1,868,782 + +0.1 1,868,789 - +0.2 1,868,790 - -0.9 1,868,792 + +1.0 1,868,823 - +1.2 1,868,836 + +1.7 1,868,845 - -1.2 1,868,876 - -4.8 1,868,886 + +0.4 1,868,886 + -0.5 1,868,894 + +1.1 1,868,894 - +1.9 1,868,896 + -2.1 1,868,896 + -1.2 1,868,904 - -0.4 1,868,904 - +0.2 1,868,904 - -2.0 1,868,941 - -1.6 1,868,943 + +1.3 1,868,943 + +2.4 1,868,943 + -0.6 1,868,943 + +1.6 1,868,943 + -0.7 1,868,951 - +2.1 1,868,951 - -0.4 1,868,951 - -1.8 1,868,961 + +0.3 1,868,961 + +0.1 1,868,961 - +0.9 1,868,966 + +2.2 1,868,999 - -3.2 1,868,999 - -1.8 1,868,999 - +3.0 1,869,137 + hypE Synpcc7942_1799 0.19 +1.0 1,869,162 + hypE Synpcc7942_1799 0.22 -1.8 1,869,165 + hypE Synpcc7942_1799 0.22 +0.2 1,869,193 - hypE Synpcc7942_1799 0.25 -0.3 1,869,195 + hypE Synpcc7942_1799 0.25 +2.5 1,869,195 + hypE Synpcc7942_1799 0.25 -1.5 1,869,203 - hypE Synpcc7942_1799 0.25 +0.4 1,869,294 - hypE Synpcc7942_1799 0.34 +2.2 1,869,302 + hypE Synpcc7942_1799 0.35 +0.9 1,869,302 + hypE Synpcc7942_1799 0.35 +2.8 1,869,310 - hypE Synpcc7942_1799 0.36 +3.1 1,869,312 + hypE Synpcc7942_1799 0.36 +1.1 1,869,312 + hypE Synpcc7942_1799 0.36 +2.0 1,869,312 + hypE Synpcc7942_1799 0.36 -3.0 1,869,312 + hypE Synpcc7942_1799 0.36 -1.1 1,869,320 - hypE Synpcc7942_1799 0.37 +0.5 1,869,320 - hypE Synpcc7942_1799 0.37 -2.7 1,869,320 - hypE Synpcc7942_1799 0.37 -3.2 1,869,320 - hypE Synpcc7942_1799 0.37 +1.8 1,869,320 - hypE Synpcc7942_1799 0.37 +0.1 1,869,340 - hypE Synpcc7942_1799 0.39 +0.1 1,869,362 - hypE Synpcc7942_1799 0.41 +0.2 1,869,462 + hypE Synpcc7942_1799 0.51 +0.9 1,869,462 + hypE Synpcc7942_1799 0.51 -1.8 1,869,470 - hypE Synpcc7942_1799 0.51 -3.7 1,869,470 - hypE Synpcc7942_1799 0.51 -2.1 1,869,488 + hypE Synpcc7942_1799 0.53 -1.1
Or see this region's nucleotide sequence