Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ldhA and Synpcc7942_1348 are separated by 17 nucleotides Synpcc7942_1348 and Synpcc7942_1349 are separated by 35 nucleotides Synpcc7942_1349 and Synpcc7942_1350 overlap by 4 nucleotides Synpcc7942_1350 and Synpcc7942_1351 are separated by 30 nucleotides
Synpcc7942_1347: ldhA - 2-hydroxyacid dehydrogenase-like, at 1,380,019 to 1,381,014
ldhA
Synpcc7942_1348: Synpcc7942_1348 - hypothetical protein, at 1,381,032 to 1,381,331
_1348
Synpcc7942_1349: Synpcc7942_1349 - hypothetical protein, at 1,381,367 to 1,382,053
_1349
Synpcc7942_1350: Synpcc7942_1350 - hypothetical protein, at 1,382,050 to 1,382,355
_1350
Synpcc7942_1351: Synpcc7942_1351 - HAD-superfamily hydrolase subfamily IA, variant 3, at 1,382,386 to 1,383,096
_1351
Position (kb) 1381
1382
1383 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 1380.375 kb on + strand, within ldhA at 1380.390 kb on - strand, within ldhA at 1380.403 kb on + strand, within ldhA at 1380.428 kb on + strand, within ldhA at 1380.448 kb on + strand at 1380.456 kb on - strand, within ldhA at 1380.461 kb on - strand, within ldhA at 1380.492 kb on + strand, within ldhA at 1380.512 kb on - strand, within ldhA at 1380.521 kb on - strand, within ldhA at 1380.540 kb on + strand, within ldhA at 1380.585 kb on + strand, within ldhA at 1380.592 kb on - strand, within ldhA at 1380.599 kb on - strand, within ldhA at 1380.599 kb on - strand, within ldhA at 1380.600 kb on - strand, within ldhA at 1380.610 kb on + strand, within ldhA at 1380.625 kb on + strand, within ldhA at 1380.647 kb on - strand, within ldhA at 1380.675 kb on + strand, within ldhA at 1380.683 kb on - strand, within ldhA at 1380.723 kb on + strand, within ldhA at 1380.729 kb on + strand, within ldhA at 1380.734 kb on + strand, within ldhA at 1380.734 kb on + strand, within ldhA at 1380.737 kb on - strand, within ldhA at 1380.737 kb on - strand, within ldhA at 1380.768 kb on - strand, within ldhA at 1380.780 kb on + strand, within ldhA at 1380.780 kb on + strand, within ldhA at 1380.785 kb on + strand, within ldhA at 1380.788 kb on - strand, within ldhA at 1380.793 kb on - strand, within ldhA at 1380.798 kb on - strand, within ldhA at 1380.808 kb on - strand, within ldhA at 1380.823 kb on - strand, within ldhA at 1380.824 kb on - strand, within ldhA at 1380.828 kb on + strand, within ldhA at 1380.828 kb on + strand, within ldhA at 1380.828 kb on + strand, within ldhA at 1380.836 kb on - strand, within ldhA at 1380.851 kb on - strand, within ldhA at 1380.863 kb on + strand, within ldhA at 1380.865 kb on + strand, within ldhA at 1380.865 kb on + strand, within ldhA at 1380.865 kb on + strand, within ldhA at 1380.873 kb on - strand, within ldhA at 1380.873 kb on - strand at 1380.873 kb on - strand, within ldhA at 1380.873 kb on - strand, within ldhA at 1380.873 kb on - strand, within ldhA at 1380.873 kb on - strand, within ldhA at 1380.875 kb on + strand, within ldhA at 1380.920 kb on + strand at 1380.981 kb on - strand at 1380.987 kb on - strand at 1380.987 kb on - strand at 1380.987 kb on - strand at 1381.030 kb on + strand at 1381.050 kb on - strand at 1381.052 kb on + strand at 1381.056 kb on + strand at 1381.060 kb on - strand at 1381.149 kb on - strand, within Synpcc7942_1348 at 1381.169 kb on + strand, within Synpcc7942_1348 at 1381.177 kb on - strand, within Synpcc7942_1348 at 1381.177 kb on - strand, within Synpcc7942_1348 at 1381.177 kb on - strand, within Synpcc7942_1348 at 1381.177 kb on - strand, within Synpcc7942_1348 at 1381.193 kb on - strand, within Synpcc7942_1348 at 1381.209 kb on - strand, within Synpcc7942_1348 at 1381.219 kb on - strand, within Synpcc7942_1348 at 1381.253 kb on - strand, within Synpcc7942_1348 at 1381.319 kb on + strand at 1381.333 kb on - strand at 1381.335 kb on + strand at 1381.343 kb on - strand at 1381.355 kb on + strand at 1381.365 kb on + strand at 1381.365 kb on + strand at 1381.370 kb on + strand at 1381.370 kb on + strand at 1381.378 kb on - strand at 1381.389 kb on + strand at 1381.400 kb on + strand at 1381.408 kb on - strand at 1381.429 kb on - strand at 1381.478 kb on - strand, within Synpcc7942_1349 at 1381.481 kb on + strand, within Synpcc7942_1349 at 1381.482 kb on - strand, within Synpcc7942_1349 at 1381.512 kb on + strand, within Synpcc7942_1349 at 1381.520 kb on - strand, within Synpcc7942_1349 at 1381.582 kb on + strand, within Synpcc7942_1349 at 1381.590 kb on - strand, within Synpcc7942_1349 at 1381.590 kb on - strand, within Synpcc7942_1349 at 1381.604 kb on + strand, within Synpcc7942_1349 at 1381.657 kb on - strand, within Synpcc7942_1349 at 1381.685 kb on - strand, within Synpcc7942_1349 at 1381.713 kb on + strand, within Synpcc7942_1349 at 1381.736 kb on - strand, within Synpcc7942_1349 at 1381.803 kb on + strand, within Synpcc7942_1349 at 1381.813 kb on + strand, within Synpcc7942_1349 at 1381.821 kb on - strand, within Synpcc7942_1349 at 1381.821 kb on - strand, within Synpcc7942_1349 at 1381.821 kb on - strand, within Synpcc7942_1349 at 1381.827 kb on + strand, within Synpcc7942_1349 at 1381.838 kb on + strand, within Synpcc7942_1349 at 1381.838 kb on + strand, within Synpcc7942_1349 at 1381.838 kb on + strand, within Synpcc7942_1349 at 1381.838 kb on + strand, within Synpcc7942_1349 at 1381.843 kb on + strand, within Synpcc7942_1349 at 1381.846 kb on - strand at 1381.851 kb on - strand, within Synpcc7942_1349 at 1381.856 kb on - strand, within Synpcc7942_1349 at 1381.883 kb on + strand, within Synpcc7942_1349 at 1381.891 kb on - strand, within Synpcc7942_1349 at 1381.897 kb on - strand, within Synpcc7942_1349 at 1381.936 kb on + strand, within Synpcc7942_1349 at 1381.947 kb on + strand, within Synpcc7942_1349 at 1381.950 kb on - strand, within Synpcc7942_1349 at 1381.952 kb on + strand, within Synpcc7942_1349 at 1381.952 kb on + strand, within Synpcc7942_1349 at 1381.955 kb on - strand, within Synpcc7942_1349 at 1381.957 kb on + strand, within Synpcc7942_1349 at 1381.957 kb on + strand, within Synpcc7942_1349 at 1381.957 kb on + strand, within Synpcc7942_1349 at 1381.972 kb on + strand, within Synpcc7942_1349 at 1381.972 kb on + strand, within Synpcc7942_1349 at 1381.994 kb on - strand at 1381.998 kb on + strand at 1381.998 kb on + strand at 1382.000 kb on - strand at 1382.008 kb on + strand at 1382.035 kb on + strand at 1382.035 kb on + strand at 1382.043 kb on - strand at 1382.076 kb on + strand at 1382.076 kb on - strand at 1382.077 kb on - strand at 1382.086 kb on - strand, within Synpcc7942_1350 at 1382.092 kb on + strand, within Synpcc7942_1350 at 1382.099 kb on - strand, within Synpcc7942_1350 at 1382.113 kb on + strand, within Synpcc7942_1350 at 1382.121 kb on - strand, within Synpcc7942_1350 at 1382.148 kb on - strand, within Synpcc7942_1350 at 1382.237 kb on + strand, within Synpcc7942_1350 at 1382.237 kb on + strand, within Synpcc7942_1350 at 1382.245 kb on - strand, within Synpcc7942_1350 at 1382.247 kb on + strand, within Synpcc7942_1350 at 1382.247 kb on + strand, within Synpcc7942_1350 at 1382.254 kb on + strand, within Synpcc7942_1350 at 1382.255 kb on - strand, within Synpcc7942_1350 at 1382.280 kb on + strand, within Synpcc7942_1350 at 1382.311 kb on + strand, within Synpcc7942_1350 at 1382.356 kb on - strand at 1382.363 kb on + strand at 1382.363 kb on + strand at 1382.363 kb on + strand at 1382.410 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 1,380,375 + ldhA Synpcc7942_1347 0.36 -1.2 1,380,390 - ldhA Synpcc7942_1347 0.37 +1.3 1,380,403 + ldhA Synpcc7942_1347 0.39 -3.8 1,380,428 + ldhA Synpcc7942_1347 0.41 +3.0 1,380,448 + -0.9 1,380,456 - ldhA Synpcc7942_1347 0.44 +2.0 1,380,461 - ldhA Synpcc7942_1347 0.44 +0.3 1,380,492 + ldhA Synpcc7942_1347 0.47 -0.0 1,380,512 - ldhA Synpcc7942_1347 0.49 +2.7 1,380,521 - ldhA Synpcc7942_1347 0.50 +0.2 1,380,540 + ldhA Synpcc7942_1347 0.52 +0.7 1,380,585 + ldhA Synpcc7942_1347 0.57 -0.9 1,380,592 - ldhA Synpcc7942_1347 0.58 -4.1 1,380,599 - ldhA Synpcc7942_1347 0.58 -1.4 1,380,599 - ldhA Synpcc7942_1347 0.58 -1.2 1,380,600 - ldhA Synpcc7942_1347 0.58 +0.1 1,380,610 + ldhA Synpcc7942_1347 0.59 -0.2 1,380,625 + ldhA Synpcc7942_1347 0.61 -2.0 1,380,647 - ldhA Synpcc7942_1347 0.63 +0.1 1,380,675 + ldhA Synpcc7942_1347 0.66 +0.1 1,380,683 - ldhA Synpcc7942_1347 0.67 +0.8 1,380,723 + ldhA Synpcc7942_1347 0.71 -2.6 1,380,729 + ldhA Synpcc7942_1347 0.71 +0.1 1,380,734 + ldhA Synpcc7942_1347 0.72 +2.8 1,380,734 + ldhA Synpcc7942_1347 0.72 -0.0 1,380,737 - ldhA Synpcc7942_1347 0.72 -0.2 1,380,737 - ldhA Synpcc7942_1347 0.72 +0.7 1,380,768 - ldhA Synpcc7942_1347 0.75 -0.0 1,380,780 + ldhA Synpcc7942_1347 0.76 +0.7 1,380,780 + ldhA Synpcc7942_1347 0.76 -0.2 1,380,785 + ldhA Synpcc7942_1347 0.77 +3.6 1,380,788 - ldhA Synpcc7942_1347 0.77 -1.7 1,380,793 - ldhA Synpcc7942_1347 0.78 +0.3 1,380,798 - ldhA Synpcc7942_1347 0.78 +0.5 1,380,808 - ldhA Synpcc7942_1347 0.79 -1.4 1,380,823 - ldhA Synpcc7942_1347 0.81 +0.4 1,380,824 - ldhA Synpcc7942_1347 0.81 +0.3 1,380,828 + ldhA Synpcc7942_1347 0.81 -0.4 1,380,828 + ldhA Synpcc7942_1347 0.81 -2.8 1,380,828 + ldhA Synpcc7942_1347 0.81 +1.9 1,380,836 - ldhA Synpcc7942_1347 0.82 +1.9 1,380,851 - ldhA Synpcc7942_1347 0.84 +2.9 1,380,863 + ldhA Synpcc7942_1347 0.85 +3.4 1,380,865 + ldhA Synpcc7942_1347 0.85 +2.5 1,380,865 + ldhA Synpcc7942_1347 0.85 +1.1 1,380,865 + ldhA Synpcc7942_1347 0.85 -4.8 1,380,873 - ldhA Synpcc7942_1347 0.86 -0.0 1,380,873 - +3.2 1,380,873 - ldhA Synpcc7942_1347 0.86 -3.3 1,380,873 - ldhA Synpcc7942_1347 0.86 -2.2 1,380,873 - ldhA Synpcc7942_1347 0.86 -2.6 1,380,873 - ldhA Synpcc7942_1347 0.86 -0.9 1,380,875 + ldhA Synpcc7942_1347 0.86 -0.3 1,380,920 + +2.7 1,380,981 - +0.1 1,380,987 - +1.8 1,380,987 - -2.8 1,380,987 - -0.6 1,381,030 + -0.9 1,381,050 - -0.6 1,381,052 + -1.4 1,381,056 + -0.2 1,381,060 - +0.3 1,381,149 - Synpcc7942_1348 0.39 +0.4 1,381,169 + Synpcc7942_1348 0.46 +0.1 1,381,177 - Synpcc7942_1348 0.48 -0.4 1,381,177 - Synpcc7942_1348 0.48 -1.9 1,381,177 - Synpcc7942_1348 0.48 +1.3 1,381,177 - Synpcc7942_1348 0.48 -3.8 1,381,193 - Synpcc7942_1348 0.54 -1.3 1,381,209 - Synpcc7942_1348 0.59 -2.9 1,381,219 - Synpcc7942_1348 0.62 -3.1 1,381,253 - Synpcc7942_1348 0.74 +0.4 1,381,319 + -2.4 1,381,333 - -2.4 1,381,335 + -2.7 1,381,343 - +0.3 1,381,355 + -2.0 1,381,365 + +0.6 1,381,365 + -1.4 1,381,370 + +1.4 1,381,370 + -0.4 1,381,378 - -2.4 1,381,389 + +2.5 1,381,400 + +3.3 1,381,408 - +1.5 1,381,429 - +2.2 1,381,478 - Synpcc7942_1349 0.16 +4.8 1,381,481 + Synpcc7942_1349 0.17 +0.5 1,381,482 - Synpcc7942_1349 0.17 +1.5 1,381,512 + Synpcc7942_1349 0.21 +4.8 1,381,520 - Synpcc7942_1349 0.22 -0.3 1,381,582 + Synpcc7942_1349 0.31 +2.8 1,381,590 - Synpcc7942_1349 0.32 +1.7 1,381,590 - Synpcc7942_1349 0.32 +1.7 1,381,604 + Synpcc7942_1349 0.34 +3.1 1,381,657 - Synpcc7942_1349 0.42 +1.2 1,381,685 - Synpcc7942_1349 0.46 +3.2 1,381,713 + Synpcc7942_1349 0.50 -0.7 1,381,736 - Synpcc7942_1349 0.54 +1.1 1,381,803 + Synpcc7942_1349 0.63 +2.2 1,381,813 + Synpcc7942_1349 0.65 +3.3 1,381,821 - Synpcc7942_1349 0.66 +2.3 1,381,821 - Synpcc7942_1349 0.66 -0.7 1,381,821 - Synpcc7942_1349 0.66 +1.4 1,381,827 + Synpcc7942_1349 0.67 +2.1 1,381,838 + Synpcc7942_1349 0.69 +5.2 1,381,838 + Synpcc7942_1349 0.69 -2.3 1,381,838 + Synpcc7942_1349 0.69 -1.7 1,381,838 + Synpcc7942_1349 0.69 -1.2 1,381,843 + Synpcc7942_1349 0.69 +3.0 1,381,846 - +0.8 1,381,851 - Synpcc7942_1349 0.70 +4.2 1,381,856 - Synpcc7942_1349 0.71 -0.2 1,381,883 + Synpcc7942_1349 0.75 +2.4 1,381,891 - Synpcc7942_1349 0.76 +2.7 1,381,897 - Synpcc7942_1349 0.77 -1.7 1,381,936 + Synpcc7942_1349 0.83 +2.4 1,381,947 + Synpcc7942_1349 0.84 +1.4 1,381,950 - Synpcc7942_1349 0.85 -1.4 1,381,952 + Synpcc7942_1349 0.85 -0.6 1,381,952 + Synpcc7942_1349 0.85 +3.0 1,381,955 - Synpcc7942_1349 0.86 +2.9 1,381,957 + Synpcc7942_1349 0.86 +1.2 1,381,957 + Synpcc7942_1349 0.86 -0.5 1,381,957 + Synpcc7942_1349 0.86 +0.9 1,381,972 + Synpcc7942_1349 0.88 +0.1 1,381,972 + Synpcc7942_1349 0.88 +0.6 1,381,994 - +2.5 1,381,998 + -1.0 1,381,998 + +1.7 1,382,000 - +3.6 1,382,008 + +3.0 1,382,035 + +1.7 1,382,035 + +2.4 1,382,043 - -0.5 1,382,076 + +2.2 1,382,076 - +0.8 1,382,077 - -2.4 1,382,086 - Synpcc7942_1350 0.12 -1.0 1,382,092 + Synpcc7942_1350 0.14 +1.0 1,382,099 - Synpcc7942_1350 0.16 +1.0 1,382,113 + Synpcc7942_1350 0.21 -0.2 1,382,121 - Synpcc7942_1350 0.23 -2.7 1,382,148 - Synpcc7942_1350 0.32 -0.6 1,382,237 + Synpcc7942_1350 0.61 -3.0 1,382,237 + Synpcc7942_1350 0.61 +0.9 1,382,245 - Synpcc7942_1350 0.64 -3.0 1,382,247 + Synpcc7942_1350 0.64 -2.8 1,382,247 + Synpcc7942_1350 0.64 -2.4 1,382,254 + Synpcc7942_1350 0.67 -1.2 1,382,255 - Synpcc7942_1350 0.67 +0.4 1,382,280 + Synpcc7942_1350 0.75 -0.1 1,382,311 + Synpcc7942_1350 0.85 +1.1 1,382,356 - -1.7 1,382,363 + -0.5 1,382,363 + -1.2 1,382,363 + -3.2 1,382,410 + -3.1
Or see this region's nucleotide sequence