Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxJ and Synpcc7942_1195 are separated by 225 nucleotides Synpcc7942_1195 and sipA are separated by 76 nucleotides sipA and trpC are separated by 306 nucleotides
Synpcc7942_1194: pdxJ - pyridoxal phosphate biosynthetic protein PdxJ, at 1,222,698 to 1,223,495
pdxJ
Synpcc7942_1195: Synpcc7942_1195 - hypothetical protein, at 1,223,721 to 1,224,221
_1195
Synpcc7942_1196: sipA - hypothetical protein, at 1,224,298 to 1,224,534
sipA
Synpcc7942_1197: trpC - indole-3-glycerol-phosphate synthase, at 1,224,841 to 1,225,728
trpC
Position (kb)
1224
1225 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1223.489 kb on + strand at 1223.489 kb on + strand at 1223.489 kb on + strand at 1223.490 kb on + strand at 1223.490 kb on + strand at 1223.492 kb on - strand at 1223.497 kb on - strand at 1223.500 kb on + strand at 1223.509 kb on + strand at 1223.538 kb on + strand at 1223.552 kb on - strand at 1223.584 kb on + strand at 1223.599 kb on - strand at 1223.608 kb on - strand at 1223.646 kb on - strand at 1223.679 kb on - strand at 1223.713 kb on - strand at 1223.718 kb on + strand at 1223.728 kb on + strand at 1223.736 kb on - strand at 1223.736 kb on - strand at 1223.737 kb on + strand at 1223.742 kb on + strand at 1223.742 kb on + strand at 1223.742 kb on + strand at 1223.750 kb on - strand at 1223.773 kb on + strand, within Synpcc7942_1195 at 1223.775 kb on - strand, within Synpcc7942_1195 at 1223.775 kb on - strand, within Synpcc7942_1195 at 1223.807 kb on - strand, within Synpcc7942_1195 at 1223.820 kb on - strand, within Synpcc7942_1195 at 1223.837 kb on - strand, within Synpcc7942_1195 at 1223.850 kb on - strand at 1223.855 kb on - strand, within Synpcc7942_1195 at 1223.863 kb on - strand, within Synpcc7942_1195 at 1223.865 kb on + strand, within Synpcc7942_1195 at 1223.873 kb on - strand, within Synpcc7942_1195 at 1223.883 kb on - strand, within Synpcc7942_1195 at 1223.898 kb on + strand, within Synpcc7942_1195 at 1223.912 kb on - strand, within Synpcc7942_1195 at 1223.948 kb on - strand, within Synpcc7942_1195 at 1223.966 kb on + strand, within Synpcc7942_1195 at 1223.982 kb on + strand, within Synpcc7942_1195 at 1223.982 kb on + strand, within Synpcc7942_1195 at 1223.989 kb on + strand, within Synpcc7942_1195 at 1223.990 kb on - strand, within Synpcc7942_1195 at 1224.019 kb on + strand, within Synpcc7942_1195 at 1224.026 kb on + strand, within Synpcc7942_1195 at 1224.026 kb on + strand, within Synpcc7942_1195 at 1224.034 kb on - strand, within Synpcc7942_1195 at 1224.036 kb on - strand, within Synpcc7942_1195 at 1224.036 kb on - strand, within Synpcc7942_1195 at 1224.076 kb on - strand, within Synpcc7942_1195 at 1224.086 kb on - strand, within Synpcc7942_1195 at 1224.111 kb on - strand, within Synpcc7942_1195 at 1224.111 kb on - strand, within Synpcc7942_1195 at 1224.145 kb on + strand, within Synpcc7942_1195 at 1224.167 kb on + strand, within Synpcc7942_1195 at 1224.279 kb on + strand at 1224.279 kb on + strand at 1224.279 kb on + strand at 1224.280 kb on - strand at 1224.287 kb on - strand at 1224.287 kb on - strand at 1224.292 kb on - strand at 1224.292 kb on - strand at 1224.432 kb on + strand, within sipA at 1224.443 kb on + strand, within sipA at 1224.454 kb on - strand, within sipA at 1224.475 kb on - strand, within sipA at 1224.475 kb on - strand, within sipA at 1224.495 kb on + strand, within sipA at 1224.529 kb on + strand at 1224.529 kb on + strand at 1224.530 kb on + strand at 1224.537 kb on - strand at 1224.537 kb on - strand at 1224.559 kb on - strand at 1224.561 kb on + strand at 1224.587 kb on - strand at 1224.593 kb on - strand at 1224.622 kb on - strand at 1224.623 kb on - strand at 1224.630 kb on - strand at 1224.663 kb on - strand at 1224.683 kb on - strand at 1224.684 kb on - strand at 1224.712 kb on + strand at 1224.716 kb on + strand at 1224.721 kb on + strand at 1224.753 kb on - strand at 1224.753 kb on - strand at 1224.754 kb on - strand at 1224.772 kb on + strand at 1224.772 kb on + strand at 1224.772 kb on + strand at 1224.792 kb on + strand at 1224.839 kb on + strand at 1224.839 kb on + strand at 1224.842 kb on - strand at 1224.846 kb on - strand at 1224.846 kb on - strand at 1224.847 kb on - strand at 1224.847 kb on - strand at 1224.853 kb on - strand at 1224.871 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 1,223,489 + -1.3 1,223,489 + -1.8 1,223,489 + -1.1 1,223,490 + -2.6 1,223,490 + -0.1 1,223,492 - -4.4 1,223,497 - -2.9 1,223,500 + -1.2 1,223,509 + -0.1 1,223,538 + -0.6 1,223,552 - -2.0 1,223,584 + -1.3 1,223,599 - +0.2 1,223,608 - -5.1 1,223,646 - -0.0 1,223,679 - -1.7 1,223,713 - +0.9 1,223,718 + -1.6 1,223,728 + -1.8 1,223,736 - -1.3 1,223,736 - -2.4 1,223,737 + -0.5 1,223,742 + -2.7 1,223,742 + -1.1 1,223,742 + -3.0 1,223,750 - -2.3 1,223,773 + Synpcc7942_1195 0.10 -0.6 1,223,775 - Synpcc7942_1195 0.11 -3.9 1,223,775 - Synpcc7942_1195 0.11 -1.3 1,223,807 - Synpcc7942_1195 0.17 -2.1 1,223,820 - Synpcc7942_1195 0.20 -2.1 1,223,837 - Synpcc7942_1195 0.23 -3.1 1,223,850 - -0.4 1,223,855 - Synpcc7942_1195 0.27 -4.5 1,223,863 - Synpcc7942_1195 0.28 -2.6 1,223,865 + Synpcc7942_1195 0.29 -0.9 1,223,873 - Synpcc7942_1195 0.30 -0.4 1,223,883 - Synpcc7942_1195 0.32 -1.0 1,223,898 + Synpcc7942_1195 0.35 -1.8 1,223,912 - Synpcc7942_1195 0.38 -2.9 1,223,948 - Synpcc7942_1195 0.45 -3.2 1,223,966 + Synpcc7942_1195 0.49 -3.8 1,223,982 + Synpcc7942_1195 0.52 -2.5 1,223,982 + Synpcc7942_1195 0.52 -0.7 1,223,989 + Synpcc7942_1195 0.53 -5.0 1,223,990 - Synpcc7942_1195 0.54 -0.8 1,224,019 + Synpcc7942_1195 0.59 -2.6 1,224,026 + Synpcc7942_1195 0.61 -2.1 1,224,026 + Synpcc7942_1195 0.61 -1.6 1,224,034 - Synpcc7942_1195 0.62 -2.6 1,224,036 - Synpcc7942_1195 0.63 -3.0 1,224,036 - Synpcc7942_1195 0.63 -3.2 1,224,076 - Synpcc7942_1195 0.71 -0.6 1,224,086 - Synpcc7942_1195 0.73 +0.1 1,224,111 - Synpcc7942_1195 0.78 -1.0 1,224,111 - Synpcc7942_1195 0.78 -4.4 1,224,145 + Synpcc7942_1195 0.85 -1.6 1,224,167 + Synpcc7942_1195 0.89 -1.1 1,224,279 + -1.2 1,224,279 + -0.4 1,224,279 + +2.0 1,224,280 - -2.6 1,224,287 - -0.1 1,224,287 - -0.1 1,224,292 - -2.6 1,224,292 - -1.0 1,224,432 + sipA Synpcc7942_1196 0.57 +4.3 1,224,443 + sipA Synpcc7942_1196 0.61 +2.7 1,224,454 - sipA Synpcc7942_1196 0.66 +3.9 1,224,475 - sipA Synpcc7942_1196 0.75 +2.7 1,224,475 - sipA Synpcc7942_1196 0.75 +2.5 1,224,495 + sipA Synpcc7942_1196 0.83 -1.6 1,224,529 + +1.1 1,224,529 + +2.2 1,224,530 + -0.7 1,224,537 - -0.7 1,224,537 - -0.5 1,224,559 - +2.3 1,224,561 + +0.4 1,224,587 - -2.8 1,224,593 - -0.7 1,224,622 - -3.5 1,224,623 - -2.8 1,224,630 - -2.5 1,224,663 - -0.3 1,224,683 - -3.4 1,224,684 - +2.6 1,224,712 + -2.7 1,224,716 + -2.4 1,224,721 + -0.7 1,224,753 - +0.5 1,224,753 - -4.6 1,224,754 - -0.6 1,224,772 + -0.4 1,224,772 + -0.6 1,224,772 + -2.2 1,224,792 + -1.3 1,224,839 + -0.3 1,224,839 + -0.6 1,224,842 - +1.0 1,224,846 - +1.1 1,224,846 - +0.5 1,224,847 - -1.2 1,224,847 - +0.5 1,224,853 - -2.4 1,224,871 + +0.3
Or see this region's nucleotide sequence