Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaA and Synpcc7942_1101 are separated by 109 nucleotides Synpcc7942_1101 and Synpcc7942_1102 are separated by 11 nucleotides Synpcc7942_1102 and Synpcc7942_1103 are separated by 64 nucleotides
Synpcc7942_1100: dnaA - chromosomal replication initiation protein, at 1,117,103 to 1,118,551
dnaA
Synpcc7942_1101: Synpcc7942_1101 - PDZ/DHR/GLGF, at 1,118,661 to 1,119,818
_1101
Synpcc7942_1102: Synpcc7942_1102 - hypothetical protein, at 1,119,830 to 1,120,258
_1102
Synpcc7942_1103: Synpcc7942_1103 - glucosamine-6-phosphate isomerase 2, at 1,120,323 to 1,121,054
_1103
Position (kb) 1118
1119
1120 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 1118.463 kb on + strand at 1118.485 kb on + strand at 1118.485 kb on + strand at 1118.493 kb on - strand at 1118.521 kb on + strand at 1118.526 kb on - strand at 1118.534 kb on - strand at 1118.595 kb on + strand at 1118.595 kb on + strand at 1118.597 kb on + strand at 1118.622 kb on + strand at 1118.642 kb on - strand at 1118.654 kb on - strand at 1118.657 kb on + strand at 1118.665 kb on - strand at 1118.667 kb on - strand at 1118.694 kb on - strand at 1118.701 kb on + strand at 1118.723 kb on + strand at 1118.728 kb on + strand at 1118.728 kb on + strand at 1118.728 kb on + strand at 1118.728 kb on + strand at 1118.728 kb on + strand at 1118.728 kb on + strand at 1118.736 kb on - strand at 1118.788 kb on + strand, within Synpcc7942_1101 at 1118.808 kb on + strand, within Synpcc7942_1101 at 1118.808 kb on + strand, within Synpcc7942_1101 at 1118.808 kb on + strand, within Synpcc7942_1101 at 1118.816 kb on - strand, within Synpcc7942_1101 at 1118.874 kb on + strand, within Synpcc7942_1101 at 1118.882 kb on - strand, within Synpcc7942_1101 at 1118.887 kb on - strand, within Synpcc7942_1101 at 1118.910 kb on + strand, within Synpcc7942_1101 at 1118.966 kb on + strand, within Synpcc7942_1101 at 1118.974 kb on + strand, within Synpcc7942_1101 at 1118.995 kb on + strand, within Synpcc7942_1101 at 1119.064 kb on - strand, within Synpcc7942_1101 at 1119.087 kb on + strand at 1119.087 kb on + strand, within Synpcc7942_1101 at 1119.087 kb on + strand, within Synpcc7942_1101 at 1119.094 kb on + strand, within Synpcc7942_1101 at 1119.095 kb on - strand, within Synpcc7942_1101 at 1119.095 kb on - strand, within Synpcc7942_1101 at 1119.111 kb on + strand, within Synpcc7942_1101 at 1119.140 kb on + strand at 1119.176 kb on + strand, within Synpcc7942_1101 at 1119.222 kb on + strand, within Synpcc7942_1101 at 1119.224 kb on + strand, within Synpcc7942_1101 at 1119.228 kb on - strand, within Synpcc7942_1101 at 1119.230 kb on - strand, within Synpcc7942_1101 at 1119.232 kb on - strand, within Synpcc7942_1101 at 1119.232 kb on - strand, within Synpcc7942_1101 at 1119.232 kb on - strand, within Synpcc7942_1101 at 1119.284 kb on - strand, within Synpcc7942_1101 at 1119.322 kb on - strand, within Synpcc7942_1101 at 1119.339 kb on - strand, within Synpcc7942_1101 at 1119.356 kb on + strand, within Synpcc7942_1101 at 1119.364 kb on - strand, within Synpcc7942_1101 at 1119.364 kb on - strand, within Synpcc7942_1101 at 1119.413 kb on + strand, within Synpcc7942_1101 at 1119.432 kb on - strand, within Synpcc7942_1101 at 1119.437 kb on - strand, within Synpcc7942_1101 at 1119.437 kb on - strand, within Synpcc7942_1101 at 1119.452 kb on - strand, within Synpcc7942_1101 at 1119.495 kb on + strand, within Synpcc7942_1101 at 1119.502 kb on - strand, within Synpcc7942_1101 at 1119.503 kb on - strand, within Synpcc7942_1101 at 1119.512 kb on - strand, within Synpcc7942_1101 at 1119.513 kb on - strand, within Synpcc7942_1101 at 1119.608 kb on - strand, within Synpcc7942_1101 at 1119.625 kb on - strand, within Synpcc7942_1101 at 1119.812 kb on + strand at 1119.815 kb on - strand at 1119.828 kb on + strand at 1119.853 kb on + strand at 1120.144 kb on - strand, within Synpcc7942_1102 at 1120.210 kb on - strand, within Synpcc7942_1102 at 1120.242 kb on - strand at 1120.254 kb on + strand at 1120.282 kb on + strand at 1120.284 kb on + strand at 1120.306 kb on + strand at 1120.314 kb on - strand at 1120.314 kb on - strand at 1120.374 kb on - strand at 1120.374 kb on - strand at 1120.374 kb on - strand at 1120.397 kb on + strand, within Synpcc7942_1103 at 1120.438 kb on - strand, within Synpcc7942_1103 at 1120.439 kb on - strand, within Synpcc7942_1103 at 1120.524 kb on - strand, within Synpcc7942_1103 at 1120.541 kb on - strand, within Synpcc7942_1103 at 1120.580 kb on + strand, within Synpcc7942_1103 at 1120.583 kb on - strand, within Synpcc7942_1103 at 1120.606 kb on - strand, within Synpcc7942_1103 at 1120.621 kb on - strand, within Synpcc7942_1103 at 1120.630 kb on - strand, within Synpcc7942_1103 at 1120.664 kb on + strand, within Synpcc7942_1103 at 1120.671 kb on + strand, within Synpcc7942_1103 at 1120.672 kb on - strand, within Synpcc7942_1103 at 1120.679 kb on - strand, within Synpcc7942_1103 at 1120.703 kb on + strand, within Synpcc7942_1103 at 1120.711 kb on - strand, within Synpcc7942_1103 at 1120.711 kb on - strand, within Synpcc7942_1103 at 1120.711 kb on - strand, within Synpcc7942_1103 at 1120.711 kb on - strand, within Synpcc7942_1103 at 1120.711 kb on - strand, within Synpcc7942_1103 at 1120.711 kb on - strand, within Synpcc7942_1103 at 1120.711 kb on - strand, within Synpcc7942_1103 at 1120.711 kb on - strand, within Synpcc7942_1103 at 1120.711 kb on - strand, within Synpcc7942_1103 at 1120.711 kb on - strand, within Synpcc7942_1103 at 1120.711 kb on - strand, within Synpcc7942_1103 at 1120.743 kb on + strand, within Synpcc7942_1103 at 1120.743 kb on + strand, within Synpcc7942_1103 at 1120.748 kb on + strand, within Synpcc7942_1103 at 1120.751 kb on - strand, within Synpcc7942_1103 at 1120.751 kb on - strand, within Synpcc7942_1103 at 1120.751 kb on - strand, within Synpcc7942_1103 at 1120.751 kb on - strand, within Synpcc7942_1103 at 1120.751 kb on - strand, within Synpcc7942_1103 at 1120.753 kb on + strand, within Synpcc7942_1103
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 1,118,463 + -1.3 1,118,485 + -1.0 1,118,485 + -2.4 1,118,493 - +1.2 1,118,521 + -0.1 1,118,526 - -3.1 1,118,534 - +1.0 1,118,595 + +1.5 1,118,595 + +0.5 1,118,597 + +0.3 1,118,622 + +1.0 1,118,642 - +4.2 1,118,654 - -0.5 1,118,657 + +1.1 1,118,665 - -1.0 1,118,667 - -1.6 1,118,694 - +0.1 1,118,701 + +1.5 1,118,723 + +2.3 1,118,728 + +0.4 1,118,728 + -1.2 1,118,728 + -1.6 1,118,728 + -0.6 1,118,728 + -0.8 1,118,728 + +1.2 1,118,736 - +0.2 1,118,788 + Synpcc7942_1101 0.11 +3.7 1,118,808 + Synpcc7942_1101 0.13 +3.8 1,118,808 + Synpcc7942_1101 0.13 +3.4 1,118,808 + Synpcc7942_1101 0.13 -0.0 1,118,816 - Synpcc7942_1101 0.13 +2.3 1,118,874 + Synpcc7942_1101 0.18 +1.7 1,118,882 - Synpcc7942_1101 0.19 -2.1 1,118,887 - Synpcc7942_1101 0.20 -0.3 1,118,910 + Synpcc7942_1101 0.22 +0.1 1,118,966 + Synpcc7942_1101 0.26 +2.8 1,118,974 + Synpcc7942_1101 0.27 +1.0 1,118,995 + Synpcc7942_1101 0.29 +2.1 1,119,064 - Synpcc7942_1101 0.35 +2.4 1,119,087 + +3.4 1,119,087 + Synpcc7942_1101 0.37 -0.2 1,119,087 + Synpcc7942_1101 0.37 -0.8 1,119,094 + Synpcc7942_1101 0.37 -0.3 1,119,095 - Synpcc7942_1101 0.37 +4.9 1,119,095 - Synpcc7942_1101 0.37 +2.2 1,119,111 + Synpcc7942_1101 0.39 +1.4 1,119,140 + +2.7 1,119,176 + Synpcc7942_1101 0.44 +2.9 1,119,222 + Synpcc7942_1101 0.48 -2.1 1,119,224 + Synpcc7942_1101 0.49 +1.5 1,119,228 - Synpcc7942_1101 0.49 +2.6 1,119,230 - Synpcc7942_1101 0.49 +1.0 1,119,232 - Synpcc7942_1101 0.49 +2.7 1,119,232 - Synpcc7942_1101 0.49 -0.0 1,119,232 - Synpcc7942_1101 0.49 +1.7 1,119,284 - Synpcc7942_1101 0.54 +0.7 1,119,322 - Synpcc7942_1101 0.57 +2.2 1,119,339 - Synpcc7942_1101 0.59 +2.1 1,119,356 + Synpcc7942_1101 0.60 +0.4 1,119,364 - Synpcc7942_1101 0.61 +1.6 1,119,364 - Synpcc7942_1101 0.61 +2.5 1,119,413 + Synpcc7942_1101 0.65 +4.6 1,119,432 - Synpcc7942_1101 0.67 +2.2 1,119,437 - Synpcc7942_1101 0.67 +0.9 1,119,437 - Synpcc7942_1101 0.67 +1.1 1,119,452 - Synpcc7942_1101 0.68 +1.6 1,119,495 + Synpcc7942_1101 0.72 +3.5 1,119,502 - Synpcc7942_1101 0.73 +0.4 1,119,503 - Synpcc7942_1101 0.73 +0.1 1,119,512 - Synpcc7942_1101 0.73 +1.2 1,119,513 - Synpcc7942_1101 0.74 -1.4 1,119,608 - Synpcc7942_1101 0.82 -0.9 1,119,625 - Synpcc7942_1101 0.83 -1.0 1,119,812 + +0.3 1,119,815 - -2.2 1,119,828 + -0.9 1,119,853 + -1.5 1,120,144 - Synpcc7942_1102 0.73 -0.5 1,120,210 - Synpcc7942_1102 0.89 -1.0 1,120,242 - -1.6 1,120,254 + -1.2 1,120,282 + -1.9 1,120,284 + -1.1 1,120,306 + +1.5 1,120,314 - +0.9 1,120,314 - +0.1 1,120,374 - -3.0 1,120,374 - +1.1 1,120,374 - +0.4 1,120,397 + Synpcc7942_1103 0.10 +1.3 1,120,438 - Synpcc7942_1103 0.16 +1.0 1,120,439 - Synpcc7942_1103 0.16 -1.6 1,120,524 - Synpcc7942_1103 0.27 -0.3 1,120,541 - Synpcc7942_1103 0.30 -0.1 1,120,580 + Synpcc7942_1103 0.35 +2.9 1,120,583 - Synpcc7942_1103 0.36 -1.5 1,120,606 - Synpcc7942_1103 0.39 -0.1 1,120,621 - Synpcc7942_1103 0.41 -0.1 1,120,630 - Synpcc7942_1103 0.42 +0.8 1,120,664 + Synpcc7942_1103 0.47 +0.9 1,120,671 + Synpcc7942_1103 0.48 +0.7 1,120,672 - Synpcc7942_1103 0.48 +3.0 1,120,679 - Synpcc7942_1103 0.49 -1.1 1,120,703 + Synpcc7942_1103 0.52 +1.5 1,120,711 - Synpcc7942_1103 0.53 -0.1 1,120,711 - Synpcc7942_1103 0.53 -2.3 1,120,711 - Synpcc7942_1103 0.53 +0.3 1,120,711 - Synpcc7942_1103 0.53 -3.0 1,120,711 - Synpcc7942_1103 0.53 +2.5 1,120,711 - Synpcc7942_1103 0.53 -1.9 1,120,711 - Synpcc7942_1103 0.53 -1.6 1,120,711 - Synpcc7942_1103 0.53 +0.7 1,120,711 - Synpcc7942_1103 0.53 +1.4 1,120,711 - Synpcc7942_1103 0.53 -0.5 1,120,711 - Synpcc7942_1103 0.53 +0.2 1,120,743 + Synpcc7942_1103 0.57 -1.4 1,120,743 + Synpcc7942_1103 0.57 +2.4 1,120,748 + Synpcc7942_1103 0.58 -0.9 1,120,751 - Synpcc7942_1103 0.58 +1.3 1,120,751 - Synpcc7942_1103 0.58 -1.0 1,120,751 - Synpcc7942_1103 0.58 -4.5 1,120,751 - Synpcc7942_1103 0.58 +0.5 1,120,751 - Synpcc7942_1103 0.58 +1.5 1,120,753 + Synpcc7942_1103 0.59 -1.0
Or see this region's nucleotide sequence