Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rplK and nusG are separated by 7 nucleotides nusG and secE are separated by 8 nucleotides secE and clpB are separated by 104 nucleotides clpB and gloA are separated by 87 nucleotides gloA and eno are separated by 213 nucleotides
Synpcc7942_0634: rplK - 50S ribosomal protein L11, at 625,866 to 626,291
rplK
Synpcc7942_0635: nusG - transcription antitermination protein NusG, at 626,299 to 626,916
nusG
Synpcc7942_0636: secE - preprotein translocase subunit SecE, at 626,925 to 627,170
secE
Synpcc7942_0637: clpB - ATPase, at 627,275 to 629,962
clpB
Synpcc7942_0638: gloA - Glyoxalase I, at 630,050 to 630,463
gloA
Synpcc7942_0639: eno - phosphopyruvate hydratase, at 630,677 to 631,969
eno
Position (kb) 627
628
629
630 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 627.281 kb on - strand at 627.330 kb on - strand at 629.968 kb on + strand at 629.968 kb on + strand at 629.968 kb on - strand at 629.976 kb on - strand at 630.019 kb on - strand at 630.021 kb on + strand at 630.026 kb on + strand at 630.041 kb on + strand at 630.041 kb on + strand at 630.049 kb on - strand at 630.073 kb on + strand at 630.081 kb on - strand at 630.091 kb on + strand at 630.154 kb on - strand, within gloA at 630.221 kb on - strand, within gloA at 630.221 kb on - strand, within gloA at 630.223 kb on + strand, within gloA at 630.238 kb on + strand, within gloA at 630.245 kb on + strand, within gloA at 630.257 kb on + strand, within gloA at 630.266 kb on + strand, within gloA at 630.284 kb on + strand at 630.287 kb on - strand, within gloA at 630.291 kb on - strand, within gloA at 630.291 kb on - strand, within gloA at 630.296 kb on + strand, within gloA at 630.303 kb on - strand, within gloA at 630.304 kb on + strand, within gloA at 630.305 kb on + strand, within gloA at 630.310 kb on + strand, within gloA at 630.333 kb on + strand, within gloA at 630.333 kb on + strand, within gloA at 630.333 kb on + strand, within gloA at 630.341 kb on - strand, within gloA at 630.351 kb on - strand, within gloA at 630.351 kb on - strand, within gloA at 630.357 kb on + strand, within gloA at 630.368 kb on - strand, within gloA at 630.397 kb on - strand, within gloA at 630.398 kb on - strand, within gloA at 630.402 kb on + strand, within gloA at 630.418 kb on - strand, within gloA at 630.449 kb on + strand at 630.452 kb on - strand at 630.503 kb on + strand at 630.511 kb on - strand at 630.518 kb on - strand at 630.539 kb on + strand at 630.541 kb on + strand at 630.549 kb on - strand at 630.549 kb on - strand at 630.591 kb on - strand at 630.666 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 627,281 - -1.0 627,330 - +0.0 629,968 + -1.0 629,968 + -0.2 629,968 - -0.3 629,976 - -2.9 630,019 - +1.2 630,021 + +0.2 630,026 + +1.3 630,041 + -0.4 630,041 + -1.8 630,049 - -0.2 630,073 + -0.2 630,081 - -1.0 630,091 + +0.0 630,154 - gloA Synpcc7942_0638 0.25 -0.4 630,221 - gloA Synpcc7942_0638 0.41 +1.6 630,221 - gloA Synpcc7942_0638 0.41 -0.9 630,223 + gloA Synpcc7942_0638 0.42 -3.6 630,238 + gloA Synpcc7942_0638 0.45 -2.7 630,245 + gloA Synpcc7942_0638 0.47 -0.6 630,257 + gloA Synpcc7942_0638 0.50 -0.7 630,266 + gloA Synpcc7942_0638 0.52 -4.4 630,284 + +1.8 630,287 - gloA Synpcc7942_0638 0.57 -1.6 630,291 - gloA Synpcc7942_0638 0.58 +1.1 630,291 - gloA Synpcc7942_0638 0.58 -0.9 630,296 + gloA Synpcc7942_0638 0.59 -1.6 630,303 - gloA Synpcc7942_0638 0.61 +1.2 630,304 + gloA Synpcc7942_0638 0.61 -1.9 630,305 + gloA Synpcc7942_0638 0.62 -0.1 630,310 + gloA Synpcc7942_0638 0.63 -0.9 630,333 + gloA Synpcc7942_0638 0.68 -0.4 630,333 + gloA Synpcc7942_0638 0.68 -0.6 630,333 + gloA Synpcc7942_0638 0.68 +2.0 630,341 - gloA Synpcc7942_0638 0.70 +0.6 630,351 - gloA Synpcc7942_0638 0.73 +1.9 630,351 - gloA Synpcc7942_0638 0.73 -1.3 630,357 + gloA Synpcc7942_0638 0.74 -1.7 630,368 - gloA Synpcc7942_0638 0.77 +0.8 630,397 - gloA Synpcc7942_0638 0.84 -1.3 630,398 - gloA Synpcc7942_0638 0.84 -1.9 630,402 + gloA Synpcc7942_0638 0.85 +0.6 630,418 - gloA Synpcc7942_0638 0.89 +2.7 630,449 + +2.6 630,452 - -2.2 630,503 + -1.1 630,511 - -2.1 630,518 - +0.7 630,539 + +0.6 630,541 + +0.9 630,549 - +0.6 630,549 - +2.9 630,591 - +0.3 630,666 - +1.2
Or see this region's nucleotide sequence