Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_645 and Pf6N2E2_646 are separated by 110 nucleotides Pf6N2E2_646 and Pf6N2E2_647 are separated by 147 nucleotides Pf6N2E2_647 and Pf6N2E2_648 are separated by 47 nucleotides Pf6N2E2_648 and Pf6N2E2_649 overlap by 4 nucleotides
Pf6N2E2_645 - General secretion pathway protein C, at 735,146 to 735,589
_645
Pf6N2E2_646 - General secretion pathway protein I, at 735,700 to 736,074
_646
Pf6N2E2_647 - General secretion pathway protein G, at 736,222 to 736,671
_647
Pf6N2E2_648 - General secretion pathway protein K, at 736,719 to 737,666
_648
Pf6N2E2_649 - General secretion pathway protein L, at 737,663 to 738,835
_649
Position (kb) 736
737 Strain fitness (log2 ratio) -2
-1
0
1
2 at 735.459 kb on + strand, within Pf6N2E2_645 at 736.159 kb on + strand at 736.171 kb on - strand at 736.171 kb on - strand at 736.171 kb on - strand at 736.519 kb on + strand, within Pf6N2E2_647 at 736.559 kb on - strand, within Pf6N2E2_647 at 736.564 kb on + strand, within Pf6N2E2_647 at 736.565 kb on - strand, within Pf6N2E2_647 at 736.670 kb on - strand at 736.670 kb on - strand at 737.400 kb on + strand, within Pf6N2E2_648 at 737.468 kb on + strand, within Pf6N2E2_648 at 737.618 kb on + strand at 737.662 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 735,459 + Pf6N2E2_645 0.70 +2.2 736,159 + -2.6 736,171 - +0.0 736,171 - -0.4 736,171 - +0.0 736,519 + Pf6N2E2_647 0.66 +0.1 736,559 - Pf6N2E2_647 0.75 -1.2 736,564 + Pf6N2E2_647 0.76 -1.0 736,565 - Pf6N2E2_647 0.76 +0.2 736,670 - +0.7 736,670 - +1.6 737,400 + Pf6N2E2_648 0.72 -0.3 737,468 + Pf6N2E2_648 0.79 +0.2 737,618 + +2.6 737,662 + -0.2
Or see this region's nucleotide sequence