Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_32 and Pf6N2E2_33 are separated by 179 nucleotides Pf6N2E2_33 and Pf6N2E2_34 are separated by 56 nucleotides
Pf6N2E2_32 - Membrane protein mosC, at 70,667 to 71,818
_32
Pf6N2E2_33 - Diaminopimelate epimerase (EC 5.1.1.7), at 71,998 to 72,999
_33
Pf6N2E2_34 - Cytosine deaminase (EC 3.5.4.1), at 73,056 to 74,303
_34
Position (kb) 71
72
73 Strain fitness (log2 ratio) -2
-1
0
1 at 71.018 kb on + strand, within Pf6N2E2_32 at 71.018 kb on + strand, within Pf6N2E2_32 at 71.019 kb on - strand, within Pf6N2E2_32 at 71.019 kb on - strand, within Pf6N2E2_32 at 71.019 kb on - strand, within Pf6N2E2_32 at 71.019 kb on - strand, within Pf6N2E2_32 at 71.073 kb on + strand, within Pf6N2E2_32 at 71.282 kb on + strand, within Pf6N2E2_32 at 71.283 kb on - strand, within Pf6N2E2_32 at 71.834 kb on + strand at 71.835 kb on - strand at 71.865 kb on + strand at 71.865 kb on + strand at 71.866 kb on - strand at 71.866 kb on - strand at 71.866 kb on - strand at 71.959 kb on + strand at 71.959 kb on + strand at 71.960 kb on - strand at 71.960 kb on - strand at 71.960 kb on - strand at 71.960 kb on - strand at 71.960 kb on - strand at 71.964 kb on + strand at 72.011 kb on - strand at 72.011 kb on - strand at 72.027 kb on + strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.031 kb on + strand at 72.031 kb on + strand at 72.031 kb on + strand at 72.031 kb on + strand at 72.032 kb on - strand at 72.032 kb on - strand at 72.255 kb on + strand, within Pf6N2E2_33 at 72.694 kb on + strand, within Pf6N2E2_33 at 72.695 kb on - strand, within Pf6N2E2_33 at 73.130 kb on - strand at 73.162 kb on + strand at 73.163 kb on - strand at 73.340 kb on + strand, within Pf6N2E2_34 at 73.458 kb on - strand, within Pf6N2E2_34 at 73.549 kb on + strand, within Pf6N2E2_34 at 73.550 kb on - strand, within Pf6N2E2_34 at 73.561 kb on + strand, within Pf6N2E2_34 at 73.562 kb on - strand, within Pf6N2E2_34 at 73.756 kb on + strand, within Pf6N2E2_34 at 73.756 kb on + strand, within Pf6N2E2_34
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 71,018 + Pf6N2E2_32 0.30 +0.0 71,018 + Pf6N2E2_32 0.30 -2.1 71,019 - Pf6N2E2_32 0.31 +0.7 71,019 - Pf6N2E2_32 0.31 -0.2 71,019 - Pf6N2E2_32 0.31 -1.1 71,019 - Pf6N2E2_32 0.31 -0.9 71,073 + Pf6N2E2_32 0.35 -0.5 71,282 + Pf6N2E2_32 0.53 +0.9 71,283 - Pf6N2E2_32 0.53 -0.1 71,834 + +0.2 71,835 - +0.7 71,865 + -0.4 71,865 + -0.3 71,866 - +0.4 71,866 - -0.4 71,866 - -0.3 71,959 + +0.5 71,959 + -0.3 71,960 - -0.5 71,960 - +0.0 71,960 - +0.4 71,960 - +0.2 71,960 - +0.9 71,964 + -2.6 72,011 - +0.3 72,011 - +0.5 72,027 + -0.1 72,028 - -0.2 72,028 - +0.1 72,028 - +0.8 72,028 - +0.1 72,028 - +0.1 72,028 - +0.5 72,028 - -0.4 72,028 - +0.4 72,031 + +0.6 72,031 + +0.2 72,031 + -0.9 72,031 + +0.0 72,032 - -0.1 72,032 - -0.2 72,255 + Pf6N2E2_33 0.26 -0.8 72,694 + Pf6N2E2_33 0.69 -0.4 72,695 - Pf6N2E2_33 0.70 +0.2 73,130 - +1.5 73,162 + -0.6 73,163 - -0.5 73,340 + Pf6N2E2_34 0.23 +0.7 73,458 - Pf6N2E2_34 0.32 -0.1 73,549 + Pf6N2E2_34 0.40 +0.3 73,550 - Pf6N2E2_34 0.40 +1.0 73,561 + Pf6N2E2_34 0.40 -0.2 73,562 - Pf6N2E2_34 0.41 +1.6 73,756 + Pf6N2E2_34 0.56 +0.8 73,756 + Pf6N2E2_34 0.56 -0.5
Or see this region's nucleotide sequence