Experiment: casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1171 and Pf6N2E2_1172 are separated by 150 nucleotides Pf6N2E2_1172 and Pf6N2E2_1173 are separated by 281 nucleotides Pf6N2E2_1173 and Pf6N2E2_1174 are separated by 278 nucleotides
Pf6N2E2_1171 - LysR family transcriptional regulator PA2877, at 1,371,260 to 1,372,165
_1171
Pf6N2E2_1172 - Short-chain dehydrogenase/reductase SDR, at 1,372,316 to 1,373,089
_1172
Pf6N2E2_1173 - hypothetical protein, at 1,373,371 to 1,373,685
_1173
Pf6N2E2_1174 - hypothetical protein, at 1,373,964 to 1,375,022
_1174
Position (kb)
1372
1373
1374 Strain fitness (log2 ratio)
-1
0
1
2 at 1371.417 kb on - strand, within Pf6N2E2_1171 at 1371.481 kb on - strand, within Pf6N2E2_1171 at 1371.481 kb on - strand, within Pf6N2E2_1171 at 1371.549 kb on + strand, within Pf6N2E2_1171 at 1371.654 kb on + strand, within Pf6N2E2_1171 at 1371.765 kb on - strand, within Pf6N2E2_1171 at 1371.765 kb on - strand, within Pf6N2E2_1171 at 1371.863 kb on - strand, within Pf6N2E2_1171 at 1372.107 kb on - strand at 1372.289 kb on + strand at 1372.290 kb on - strand at 1372.290 kb on - strand at 1372.317 kb on + strand at 1372.317 kb on + strand at 1372.318 kb on - strand at 1372.318 kb on - strand at 1372.330 kb on - strand at 1372.330 kb on - strand at 1372.330 kb on - strand at 1372.330 kb on - strand at 1372.382 kb on + strand at 1372.382 kb on + strand at 1372.382 kb on + strand at 1372.382 kb on + strand at 1372.382 kb on + strand at 1372.383 kb on - strand at 1372.383 kb on - strand at 1372.393 kb on + strand at 1372.394 kb on - strand, within Pf6N2E2_1172 at 1372.403 kb on + strand, within Pf6N2E2_1172 at 1372.403 kb on + strand, within Pf6N2E2_1172 at 1372.403 kb on + strand, within Pf6N2E2_1172 at 1372.403 kb on + strand, within Pf6N2E2_1172 at 1372.404 kb on - strand at 1372.404 kb on - strand, within Pf6N2E2_1172 at 1372.404 kb on - strand, within Pf6N2E2_1172 at 1372.404 kb on - strand, within Pf6N2E2_1172 at 1372.511 kb on - strand, within Pf6N2E2_1172 at 1372.614 kb on + strand, within Pf6N2E2_1172 at 1372.614 kb on + strand, within Pf6N2E2_1172 at 1372.623 kb on + strand, within Pf6N2E2_1172 at 1372.654 kb on + strand, within Pf6N2E2_1172 at 1372.654 kb on + strand, within Pf6N2E2_1172 at 1372.654 kb on + strand, within Pf6N2E2_1172 at 1372.655 kb on - strand, within Pf6N2E2_1172 at 1372.655 kb on - strand, within Pf6N2E2_1172 at 1372.655 kb on - strand, within Pf6N2E2_1172 at 1372.655 kb on - strand, within Pf6N2E2_1172 at 1372.655 kb on - strand, within Pf6N2E2_1172 at 1372.658 kb on + strand, within Pf6N2E2_1172 at 1372.658 kb on + strand, within Pf6N2E2_1172 at 1372.658 kb on + strand, within Pf6N2E2_1172 at 1372.659 kb on - strand, within Pf6N2E2_1172 at 1372.954 kb on + strand, within Pf6N2E2_1172 at 1372.954 kb on + strand, within Pf6N2E2_1172 at 1372.954 kb on + strand, within Pf6N2E2_1172 at 1372.955 kb on - strand, within Pf6N2E2_1172 at 1372.971 kb on + strand, within Pf6N2E2_1172 at 1373.066 kb on - strand at 1373.066 kb on - strand at 1373.107 kb on - strand at 1373.107 kb on - strand at 1373.129 kb on + strand at 1373.130 kb on - strand at 1373.161 kb on + strand at 1373.228 kb on + strand at 1373.285 kb on - strand at 1373.288 kb on + strand at 1373.472 kb on - strand, within Pf6N2E2_1173 at 1373.472 kb on - strand, within Pf6N2E2_1173 at 1373.518 kb on + strand, within Pf6N2E2_1173 at 1373.519 kb on - strand, within Pf6N2E2_1173 at 1373.519 kb on - strand, within Pf6N2E2_1173 at 1373.725 kb on + strand at 1373.791 kb on - strand at 1373.965 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos (C) remove 1,371,417 - Pf6N2E2_1171 0.17 +0.2 1,371,481 - Pf6N2E2_1171 0.24 +1.1 1,371,481 - Pf6N2E2_1171 0.24 +0.1 1,371,549 + Pf6N2E2_1171 0.32 +0.7 1,371,654 + Pf6N2E2_1171 0.43 -0.9 1,371,765 - Pf6N2E2_1171 0.56 -0.1 1,371,765 - Pf6N2E2_1171 0.56 +1.3 1,371,863 - Pf6N2E2_1171 0.67 +1.0 1,372,107 - -0.5 1,372,289 + +0.8 1,372,290 - +0.4 1,372,290 - +0.2 1,372,317 + +0.3 1,372,317 + -0.9 1,372,318 - +0.3 1,372,318 - -0.3 1,372,330 - +1.3 1,372,330 - +0.5 1,372,330 - -1.1 1,372,330 - -0.8 1,372,382 + +0.7 1,372,382 + +0.6 1,372,382 + +0.0 1,372,382 + -1.2 1,372,382 + -0.5 1,372,383 - -0.1 1,372,383 - +0.2 1,372,393 + -0.2 1,372,394 - Pf6N2E2_1172 0.10 +0.8 1,372,403 + Pf6N2E2_1172 0.11 +0.6 1,372,403 + Pf6N2E2_1172 0.11 +0.7 1,372,403 + Pf6N2E2_1172 0.11 -0.2 1,372,403 + Pf6N2E2_1172 0.11 +0.7 1,372,404 - +1.4 1,372,404 - Pf6N2E2_1172 0.11 +1.4 1,372,404 - Pf6N2E2_1172 0.11 +0.7 1,372,404 - Pf6N2E2_1172 0.11 -0.3 1,372,511 - Pf6N2E2_1172 0.25 +1.0 1,372,614 + Pf6N2E2_1172 0.39 +0.6 1,372,614 + Pf6N2E2_1172 0.39 -0.4 1,372,623 + Pf6N2E2_1172 0.40 +0.8 1,372,654 + Pf6N2E2_1172 0.44 +0.6 1,372,654 + Pf6N2E2_1172 0.44 +0.7 1,372,654 + Pf6N2E2_1172 0.44 +0.5 1,372,655 - Pf6N2E2_1172 0.44 +0.6 1,372,655 - Pf6N2E2_1172 0.44 +0.4 1,372,655 - Pf6N2E2_1172 0.44 +1.1 1,372,655 - Pf6N2E2_1172 0.44 +1.6 1,372,655 - Pf6N2E2_1172 0.44 +0.8 1,372,658 + Pf6N2E2_1172 0.44 +2.3 1,372,658 + Pf6N2E2_1172 0.44 +0.6 1,372,658 + Pf6N2E2_1172 0.44 -0.6 1,372,659 - Pf6N2E2_1172 0.44 +1.0 1,372,954 + Pf6N2E2_1172 0.82 +0.7 1,372,954 + Pf6N2E2_1172 0.82 +0.2 1,372,954 + Pf6N2E2_1172 0.82 +0.6 1,372,955 - Pf6N2E2_1172 0.83 -0.5 1,372,971 + Pf6N2E2_1172 0.85 +0.6 1,373,066 - +0.5 1,373,066 - -0.2 1,373,107 - +0.4 1,373,107 - +0.5 1,373,129 + +0.9 1,373,130 - +1.2 1,373,161 + +0.1 1,373,228 + +1.0 1,373,285 - +0.2 1,373,288 + -0.4 1,373,472 - Pf6N2E2_1173 0.32 +0.9 1,373,472 - Pf6N2E2_1173 0.32 -0.4 1,373,518 + Pf6N2E2_1173 0.47 -1.2 1,373,519 - Pf6N2E2_1173 0.47 +0.6 1,373,519 - Pf6N2E2_1173 0.47 -0.4 1,373,725 + -0.2 1,373,791 - -0.6 1,373,965 + -0.1
Or see this region's nucleotide sequence