Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01405 and MMJJ_RS01410 are separated by 95 nucleotides MMJJ_RS01410 and MMJJ_RS01415 are separated by 134 nucleotides MMJJ_RS01415 and MMJJ_RS01420 are separated by 81 nucleotides
MMJJ_RS01405: MMJJ_RS01405 - proline--tRNA ligase, at 272,996 to 274,378
_RS01405
MMJJ_RS01410: MMJJ_RS01410 - archaeal proteasome endopeptidase complex subunit beta, at 274,474 to 275,133
_RS01410
MMJJ_RS01415: MMJJ_RS01415 - beta-CASP ribonuclease aCPSF1, at 275,268 to 277,175
_RS01415
MMJJ_RS01420: MMJJ_RS01420 - hypothetical protein, at 277,257 to 278,123
_RS01420
Position (kb) 275
276
277
278 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 274.376 kb on - strand at 274.376 kb on - strand at 274.376 kb on - strand at 274.376 kb on - strand at 274.377 kb on + strand at 274.377 kb on + strand at 274.377 kb on + strand at 274.377 kb on + strand at 274.377 kb on + strand at 274.414 kb on + strand at 274.428 kb on + strand at 274.428 kb on + strand at 274.428 kb on + strand at 274.430 kb on + strand at 274.430 kb on + strand at 274.430 kb on + strand at 274.430 kb on + strand at 274.441 kb on + strand at 274.445 kb on - strand at 274.446 kb on + strand at 274.446 kb on + strand at 274.446 kb on + strand at 274.446 kb on + strand at 274.457 kb on - strand at 274.458 kb on + strand at 274.460 kb on - strand at 274.460 kb on - strand at 274.460 kb on - strand at 274.461 kb on + strand at 274.471 kb on + strand at 274.493 kb on + strand at 274.504 kb on - strand at 274.504 kb on - strand at 274.504 kb on - strand at 274.505 kb on + strand at 274.505 kb on + strand at 274.505 kb on + strand at 274.532 kb on - strand at 274.532 kb on - strand at 274.557 kb on + strand, within MMJJ_RS01410 at 274.557 kb on + strand, within MMJJ_RS01410 at 274.619 kb on + strand, within MMJJ_RS01410 at 274.619 kb on + strand, within MMJJ_RS01410 at 274.621 kb on + strand, within MMJJ_RS01410 at 274.621 kb on + strand, within MMJJ_RS01410 at 274.633 kb on - strand, within MMJJ_RS01410 at 274.638 kb on + strand, within MMJJ_RS01410 at 274.647 kb on - strand, within MMJJ_RS01410 at 274.649 kb on - strand, within MMJJ_RS01410 at 274.650 kb on + strand, within MMJJ_RS01410 at 274.650 kb on + strand, within MMJJ_RS01410 at 274.735 kb on + strand, within MMJJ_RS01410 at 274.760 kb on - strand, within MMJJ_RS01410 at 274.767 kb on - strand, within MMJJ_RS01410 at 274.811 kb on - strand, within MMJJ_RS01410 at 274.811 kb on - strand, within MMJJ_RS01410 at 274.811 kb on - strand, within MMJJ_RS01410 at 275.026 kb on - strand, within MMJJ_RS01410 at 275.232 kb on + strand at 275.249 kb on + strand at 275.368 kb on - strand at 275.983 kb on - strand, within MMJJ_RS01415 at 276.192 kb on - strand, within MMJJ_RS01415 at 276.203 kb on - strand, within MMJJ_RS01415 at 276.422 kb on + strand at 276.463 kb on - strand, within MMJJ_RS01415 at 276.779 kb on - strand at 276.857 kb on + strand, within MMJJ_RS01415 at 276.947 kb on - strand, within MMJJ_RS01415 at 277.177 kb on - strand at 277.232 kb on - strand at 277.233 kb on + strand at 277.233 kb on + strand at 277.233 kb on + strand at 277.234 kb on - strand at 277.234 kb on - strand at 277.235 kb on + strand at 277.240 kb on - strand at 277.240 kb on - strand at 277.240 kb on - strand at 277.241 kb on + strand at 277.253 kb on - strand at 277.254 kb on + strand at 277.264 kb on + strand at 277.330 kb on - strand at 277.331 kb on + strand at 277.365 kb on + strand, within MMJJ_RS01420 at 277.380 kb on - strand, within MMJJ_RS01420 at 277.389 kb on - strand, within MMJJ_RS01420 at 277.389 kb on - strand, within MMJJ_RS01420 at 277.390 kb on + strand, within MMJJ_RS01420 at 277.390 kb on + strand, within MMJJ_RS01420 at 277.397 kb on - strand, within MMJJ_RS01420 at 277.398 kb on + strand, within MMJJ_RS01420 at 277.398 kb on + strand, within MMJJ_RS01420 at 277.399 kb on - strand, within MMJJ_RS01420 at 277.399 kb on - strand, within MMJJ_RS01420 at 277.400 kb on + strand, within MMJJ_RS01420 at 277.400 kb on + strand, within MMJJ_RS01420 at 277.403 kb on - strand, within MMJJ_RS01420 at 277.403 kb on - strand, within MMJJ_RS01420 at 277.445 kb on + strand, within MMJJ_RS01420 at 277.506 kb on - strand, within MMJJ_RS01420 at 277.519 kb on - strand, within MMJJ_RS01420 at 277.534 kb on - strand, within MMJJ_RS01420 at 277.549 kb on - strand, within MMJJ_RS01420 at 277.551 kb on - strand, within MMJJ_RS01420 at 277.551 kb on - strand, within MMJJ_RS01420 at 277.552 kb on + strand, within MMJJ_RS01420 at 277.562 kb on - strand, within MMJJ_RS01420 at 277.563 kb on + strand, within MMJJ_RS01420 at 277.563 kb on + strand, within MMJJ_RS01420 at 277.563 kb on + strand, within MMJJ_RS01420 at 277.566 kb on - strand, within MMJJ_RS01420 at 277.586 kb on + strand, within MMJJ_RS01420 at 277.623 kb on + strand, within MMJJ_RS01420 at 277.635 kb on - strand, within MMJJ_RS01420 at 277.825 kb on - strand, within MMJJ_RS01420 at 277.826 kb on + strand, within MMJJ_RS01420 at 277.830 kb on + strand, within MMJJ_RS01420 at 277.860 kb on + strand, within MMJJ_RS01420 at 277.889 kb on + strand, within MMJJ_RS01420 at 277.961 kb on + strand, within MMJJ_RS01420 at 277.961 kb on + strand, within MMJJ_RS01420 at 277.964 kb on - strand, within MMJJ_RS01420 at 277.987 kb on - strand, within MMJJ_RS01420 at 277.987 kb on - strand, within MMJJ_RS01420 at 277.988 kb on + strand, within MMJJ_RS01420 at 277.998 kb on - strand, within MMJJ_RS01420 at 278.029 kb on - strand, within MMJJ_RS01420 at 278.038 kb on + strand at 278.085 kb on - strand at 278.086 kb on + strand at 278.103 kb on + strand at 278.108 kb on - strand at 278.113 kb on + strand at 278.130 kb on - strand at 278.133 kb on - strand at 278.133 kb on - strand at 278.133 kb on - strand at 278.133 kb on - strand at 278.134 kb on + strand at 278.135 kb on - strand at 278.135 kb on - strand at 278.135 kb on - strand at 278.136 kb on + strand at 278.136 kb on + strand at 278.136 kb on + strand at 278.136 kb on + strand at 278.136 kb on + strand at 278.137 kb on - strand at 278.138 kb on + strand at 278.138 kb on + strand at 278.141 kb on + strand at 278.141 kb on + strand at 278.148 kb on + strand at 278.150 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE remove 274,376 - -0.1 274,376 - -0.2 274,376 - -0.3 274,376 - +0.3 274,377 + -0.1 274,377 + -0.9 274,377 + -0.3 274,377 + -0.2 274,377 + +0.1 274,414 + -0.4 274,428 + -0.4 274,428 + +0.6 274,428 + -0.5 274,430 + -0.9 274,430 + -1.7 274,430 + -0.1 274,430 + -0.8 274,441 + -0.7 274,445 - -0.5 274,446 + -0.9 274,446 + -0.4 274,446 + -1.1 274,446 + -0.2 274,457 - -0.7 274,458 + -1.0 274,460 - -0.7 274,460 - -0.3 274,460 - +0.2 274,461 + -0.8 274,471 + -1.3 274,493 + -0.8 274,504 - -0.7 274,504 - -0.6 274,504 - -0.7 274,505 + -0.1 274,505 + -0.8 274,505 + -1.5 274,532 - -0.1 274,532 - -0.6 274,557 + MMJJ_RS01410 0.13 -0.1 274,557 + MMJJ_RS01410 0.13 -0.8 274,619 + MMJJ_RS01410 0.22 -2.9 274,619 + MMJJ_RS01410 0.22 -0.4 274,621 + MMJJ_RS01410 0.22 -2.4 274,621 + MMJJ_RS01410 0.22 -0.1 274,633 - MMJJ_RS01410 0.24 +0.3 274,638 + MMJJ_RS01410 0.25 -0.1 274,647 - MMJJ_RS01410 0.26 -1.7 274,649 - MMJJ_RS01410 0.27 +0.9 274,650 + MMJJ_RS01410 0.27 -2.8 274,650 + MMJJ_RS01410 0.27 -0.9 274,735 + MMJJ_RS01410 0.40 -2.3 274,760 - MMJJ_RS01410 0.43 -0.4 274,767 - MMJJ_RS01410 0.44 -2.4 274,811 - MMJJ_RS01410 0.51 -0.9 274,811 - MMJJ_RS01410 0.51 -0.4 274,811 - MMJJ_RS01410 0.51 -1.4 275,026 - MMJJ_RS01410 0.84 -1.1 275,232 + -1.9 275,249 + -2.7 275,368 - -2.1 275,983 - MMJJ_RS01415 0.37 -2.4 276,192 - MMJJ_RS01415 0.48 -1.9 276,203 - MMJJ_RS01415 0.49 -1.4 276,422 + -1.1 276,463 - MMJJ_RS01415 0.63 -1.3 276,779 - -1.1 276,857 + MMJJ_RS01415 0.83 -1.4 276,947 - MMJJ_RS01415 0.88 -1.9 277,177 - +0.5 277,232 - +0.4 277,233 + -0.2 277,233 + -0.2 277,233 + -0.5 277,234 - -0.8 277,234 - -0.1 277,235 + +0.0 277,240 - +0.0 277,240 - +0.9 277,240 - +0.8 277,241 + -0.1 277,253 - -0.3 277,254 + +0.4 277,264 + -1.3 277,330 - -0.5 277,331 + +0.5 277,365 + MMJJ_RS01420 0.12 +0.2 277,380 - MMJJ_RS01420 0.14 -0.6 277,389 - MMJJ_RS01420 0.15 +0.7 277,389 - MMJJ_RS01420 0.15 -0.5 277,390 + MMJJ_RS01420 0.15 -0.5 277,390 + MMJJ_RS01420 0.15 +0.1 277,397 - MMJJ_RS01420 0.16 +0.1 277,398 + MMJJ_RS01420 0.16 +0.5 277,398 + MMJJ_RS01420 0.16 -1.0 277,399 - MMJJ_RS01420 0.16 +0.2 277,399 - MMJJ_RS01420 0.16 -0.4 277,400 + MMJJ_RS01420 0.16 +1.1 277,400 + MMJJ_RS01420 0.16 +0.9 277,403 - MMJJ_RS01420 0.17 -1.1 277,403 - MMJJ_RS01420 0.17 +0.1 277,445 + MMJJ_RS01420 0.22 -0.5 277,506 - MMJJ_RS01420 0.29 +0.0 277,519 - MMJJ_RS01420 0.30 -0.6 277,534 - MMJJ_RS01420 0.32 +0.4 277,549 - MMJJ_RS01420 0.34 +0.2 277,551 - MMJJ_RS01420 0.34 -1.0 277,551 - MMJJ_RS01420 0.34 +0.7 277,552 + MMJJ_RS01420 0.34 +0.0 277,562 - MMJJ_RS01420 0.35 -0.4 277,563 + MMJJ_RS01420 0.35 +0.4 277,563 + MMJJ_RS01420 0.35 -1.1 277,563 + MMJJ_RS01420 0.35 -0.4 277,566 - MMJJ_RS01420 0.36 +0.6 277,586 + MMJJ_RS01420 0.38 +0.5 277,623 + MMJJ_RS01420 0.42 +0.5 277,635 - MMJJ_RS01420 0.44 +0.2 277,825 - MMJJ_RS01420 0.66 +0.3 277,826 + MMJJ_RS01420 0.66 -0.3 277,830 + MMJJ_RS01420 0.66 +0.0 277,860 + MMJJ_RS01420 0.70 -0.8 277,889 + MMJJ_RS01420 0.73 +0.3 277,961 + MMJJ_RS01420 0.81 -0.4 277,961 + MMJJ_RS01420 0.81 -0.1 277,964 - MMJJ_RS01420 0.82 +0.5 277,987 - MMJJ_RS01420 0.84 +0.6 277,987 - MMJJ_RS01420 0.84 +0.0 277,988 + MMJJ_RS01420 0.84 +0.8 277,998 - MMJJ_RS01420 0.85 +0.5 278,029 - MMJJ_RS01420 0.89 +0.4 278,038 + +0.0 278,085 - -0.2 278,086 + -0.4 278,103 + +0.2 278,108 - +0.4 278,113 + +0.2 278,130 - -0.1 278,133 - +0.9 278,133 - +0.3 278,133 - +0.4 278,133 - -1.3 278,134 + +0.3 278,135 - -0.7 278,135 - -0.5 278,135 - -0.4 278,136 + +0.1 278,136 + +0.0 278,136 + -0.5 278,136 + -1.1 278,136 + -0.4 278,137 - -0.0 278,138 + +0.4 278,138 + +0.2 278,141 + -0.0 278,141 + -0.7 278,148 + +0.5 278,150 - +0.3
Or see this region's nucleotide sequence