Experiment: CDM1 without Pantothenate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15855 and BBR_RS15860 are separated by 157 nucleotides BBR_RS15860 and BBR_RS15865 are separated by 40 nucleotides BBR_RS15865 and BBR_RS15870 are separated by 135 nucleotides BBR_RS15870 and BBR_RS20940 are separated by 28 nucleotides BBR_RS20940 and BBR_RS20945 overlap by 59 nucleotides BBR_RS20945 and BBR_RS15875 are separated by 31 nucleotides
BBR_RS15855: BBR_RS15855 - N-acetyltransferase, at 1,322,358 to 1,322,873
_RS15855
BBR_RS15860: BBR_RS15860 - DUF2316 domain-containing protein, at 1,323,031 to 1,323,213
_RS15860
BBR_RS15865: BBR_RS15865 - GNAT family N-acetyltransferase, at 1,323,254 to 1,323,787
_RS15865
BBR_RS15870: BBR_RS15870 - hypothetical protein, at 1,323,923 to 1,324,252
_RS15870
BBR_RS20940: BBR_RS20940 - hypothetical protein, at 1,324,281 to 1,324,487
_RS20940
BBR_RS20945: BBR_RS20945 - hypothetical protein, at 1,324,429 to 1,324,630
_RS20945
BBR_RS15875: BBR_RS15875 - DNA-binding protein, at 1,324,662 to 1,325,468
_RS15875
Position (kb) 1323
1324 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1322.355 kb on + strand at 1322.421 kb on + strand, within BBR_RS15855 at 1322.421 kb on + strand, within BBR_RS15855 at 1322.426 kb on + strand, within BBR_RS15855 at 1322.523 kb on + strand, within BBR_RS15855 at 1322.621 kb on + strand, within BBR_RS15855 at 1322.629 kb on - strand, within BBR_RS15855 at 1322.706 kb on - strand, within BBR_RS15855 at 1322.801 kb on - strand, within BBR_RS15855 at 1322.885 kb on - strand at 1322.964 kb on - strand at 1322.977 kb on + strand at 1323.029 kb on + strand at 1323.037 kb on - strand at 1323.106 kb on + strand, within BBR_RS15860 at 1323.109 kb on + strand, within BBR_RS15860 at 1323.216 kb on - strand at 1323.294 kb on - strand at 1323.302 kb on - strand at 1323.332 kb on + strand, within BBR_RS15865 at 1323.361 kb on + strand, within BBR_RS15865 at 1323.403 kb on - strand, within BBR_RS15865 at 1323.404 kb on - strand, within BBR_RS15865 at 1323.414 kb on - strand, within BBR_RS15865 at 1323.472 kb on - strand, within BBR_RS15865 at 1323.505 kb on + strand, within BBR_RS15865 at 1323.505 kb on + strand, within BBR_RS15865 at 1323.513 kb on - strand, within BBR_RS15865 at 1323.630 kb on - strand, within BBR_RS15865 at 1323.739 kb on + strand at 1323.771 kb on + strand at 1323.806 kb on + strand at 1323.814 kb on - strand at 1323.831 kb on - strand at 1323.852 kb on + strand at 1323.860 kb on - strand at 1323.881 kb on - strand at 1323.911 kb on + strand at 1323.919 kb on - strand at 1323.967 kb on - strand, within BBR_RS15870 at 1323.979 kb on - strand, within BBR_RS15870 at 1323.989 kb on + strand, within BBR_RS15870 at 1323.997 kb on - strand, within BBR_RS15870 at 1323.997 kb on - strand, within BBR_RS15870 at 1324.029 kb on + strand, within BBR_RS15870 at 1324.159 kb on + strand, within BBR_RS15870 at 1324.205 kb on - strand, within BBR_RS15870 at 1324.247 kb on + strand at 1324.255 kb on - strand at 1324.269 kb on + strand at 1324.762 kb on + strand, within BBR_RS15875 at 1324.764 kb on + strand, within BBR_RS15875 at 1324.767 kb on + strand, within BBR_RS15875
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Pantothenate remove 1,322,355 + +0.0 1,322,421 + BBR_RS15855 0.12 -0.8 1,322,421 + BBR_RS15855 0.12 -0.6 1,322,426 + BBR_RS15855 0.13 +1.7 1,322,523 + BBR_RS15855 0.32 +0.0 1,322,621 + BBR_RS15855 0.51 -0.1 1,322,629 - BBR_RS15855 0.53 -1.3 1,322,706 - BBR_RS15855 0.67 +0.4 1,322,801 - BBR_RS15855 0.86 +0.9 1,322,885 - -0.4 1,322,964 - -1.2 1,322,977 + +1.8 1,323,029 + -0.0 1,323,037 - +0.8 1,323,106 + BBR_RS15860 0.41 -0.0 1,323,109 + BBR_RS15860 0.43 +0.1 1,323,216 - -1.0 1,323,294 - -1.3 1,323,302 - +0.3 1,323,332 + BBR_RS15865 0.15 -1.5 1,323,361 + BBR_RS15865 0.20 +0.8 1,323,403 - BBR_RS15865 0.28 -1.1 1,323,404 - BBR_RS15865 0.28 -1.9 1,323,414 - BBR_RS15865 0.30 -1.5 1,323,472 - BBR_RS15865 0.41 -2.5 1,323,505 + BBR_RS15865 0.47 -0.2 1,323,505 + BBR_RS15865 0.47 -3.1 1,323,513 - BBR_RS15865 0.49 +1.0 1,323,630 - BBR_RS15865 0.70 -0.6 1,323,739 + -0.5 1,323,771 + +0.2 1,323,806 + +0.1 1,323,814 - -0.8 1,323,831 - +0.3 1,323,852 + -0.2 1,323,860 - -0.2 1,323,881 - -0.4 1,323,911 + -2.2 1,323,919 - -0.1 1,323,967 - BBR_RS15870 0.13 -0.8 1,323,979 - BBR_RS15870 0.17 -0.1 1,323,989 + BBR_RS15870 0.20 -1.5 1,323,997 - BBR_RS15870 0.22 -0.6 1,323,997 - BBR_RS15870 0.22 -1.7 1,324,029 + BBR_RS15870 0.32 +0.0 1,324,159 + BBR_RS15870 0.72 -0.9 1,324,205 - BBR_RS15870 0.85 -0.5 1,324,247 + -2.7 1,324,255 - +1.2 1,324,269 + -2.0 1,324,762 + BBR_RS15875 0.12 -2.4 1,324,764 + BBR_RS15875 0.13 -1.8 1,324,767 + BBR_RS15875 0.13 +0.3
Or see this region's nucleotide sequence