Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2160 and Shewana3_2161 are separated by 188 nucleotides Shewana3_2161 and Shewana3_2162 are separated by 249 nucleotides Shewana3_2162 and Shewana3_R0092 are separated by 125 nucleotides Shewana3_R0092 and Shewana3_R0093 are separated by 39 nucleotides
Shewana3_2160: Shewana3_2160 - diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq), at 2,587,987 to 2,590,170
_2160
Shewana3_2161: Shewana3_2161 - hypothetical protein (RefSeq), at 2,590,359 to 2,590,646
_2161
Shewana3_2162: Shewana3_2162 - hypothetical protein (RefSeq), at 2,590,896 to 2,591,384
_2162
Shewana3_R0092: Shewana3_R0092 - tRNA-Tyr (RefSeq), at 2,591,510 to 2,591,594
_R0092
Shewana3_R0093: Shewana3_R0093 - tRNA-Tyr (RefSeq), at 2,591,634 to 2,591,718
_R0093
Position (kb)
2590
2591 Strain fitness (log2 ratio)
-1
0
1
2 at 2589.374 kb on - strand, within Shewana3_2160 at 2589.419 kb on + strand, within Shewana3_2160 at 2589.612 kb on - strand, within Shewana3_2160 at 2589.841 kb on - strand at 2589.868 kb on - strand, within Shewana3_2160 at 2589.946 kb on - strand, within Shewana3_2160 at 2590.174 kb on + strand at 2590.350 kb on + strand at 2590.358 kb on - strand at 2590.515 kb on - strand, within Shewana3_2161 at 2590.675 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 2,589,374 - Shewana3_2160 0.64 +1.4 2,589,419 + Shewana3_2160 0.66 +2.0 2,589,612 - Shewana3_2160 0.74 +0.1 2,589,841 - +1.0 2,589,868 - Shewana3_2160 0.86 +1.0 2,589,946 - Shewana3_2160 0.90 +0.8 2,590,174 + -1.0 2,590,350 + -0.5 2,590,358 - +0.1 2,590,515 - Shewana3_2161 0.54 -1.2 2,590,675 - +0.3
Or see this region's nucleotide sequence