Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1960 and Shewana3_1961 are separated by 8 nucleotides Shewana3_1961 and Shewana3_1962 overlap by 4 nucleotides Shewana3_1962 and ansA are separated by 70 nucleotides ansA and Shewana3_1964 are separated by 111 nucleotides
Shewana3_1960: Shewana3_1960 - peptidase M14, carboxypeptidase A (RefSeq), at 2,333,947 to 2,335,074
_1960
Shewana3_1961: Shewana3_1961 - hypothetical protein (RefSeq), at 2,335,083 to 2,335,400
_1961
Shewana3_1962: Shewana3_1962 - hypothetical protein (RefSeq), at 2,335,397 to 2,335,678
_1962
Shewana3_1963: ansA - cytoplasmic asparaginase I (RefSeq), at 2,335,749 to 2,336,762
ansA
Shewana3_1964: Shewana3_1964 - signal peptide peptidase SppA, 67K type (RefSeq), at 2,336,874 to 2,338,718
_1964
Position (kb)
2335
2336
2337 Strain fitness (log2 ratio)
-2
-1
0
1 at 2334.952 kb on - strand, within Shewana3_1960 at 2335.024 kb on + strand at 2335.558 kb on + strand, within Shewana3_1962 at 2335.845 kb on - strand at 2335.938 kb on - strand, within ansA at 2336.041 kb on - strand, within ansA at 2336.150 kb on - strand, within ansA at 2336.215 kb on + strand, within ansA at 2336.463 kb on + strand at 2336.541 kb on - strand, within ansA at 2336.760 kb on + strand at 2336.920 kb on - strand at 2336.981 kb on + strand at 2337.126 kb on + strand, within Shewana3_1964 at 2337.500 kb on + strand, within Shewana3_1964
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 2,334,952 - Shewana3_1960 0.89 -2.1 2,335,024 + -0.4 2,335,558 + Shewana3_1962 0.57 +0.2 2,335,845 - -1.4 2,335,938 - ansA Shewana3_1963 0.19 -1.2 2,336,041 - ansA Shewana3_1963 0.29 -0.8 2,336,150 - ansA Shewana3_1963 0.40 -0.1 2,336,215 + ansA Shewana3_1963 0.46 -0.7 2,336,463 + -0.4 2,336,541 - ansA Shewana3_1963 0.78 -1.4 2,336,760 + -2.2 2,336,920 - +0.2 2,336,981 + +1.4 2,337,126 + Shewana3_1964 0.14 +1.4 2,337,500 + Shewana3_1964 0.34 -1.8
Or see this region's nucleotide sequence