Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1963

Experiment: L-Proline (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShewana3_1960 and Shewana3_1961 are separated by 8 nucleotidesShewana3_1961 and Shewana3_1962 overlap by 4 nucleotidesShewana3_1962 and ansA are separated by 70 nucleotidesansA and Shewana3_1964 are separated by 111 nucleotides Shewana3_1960: Shewana3_1960 - peptidase M14, carboxypeptidase A (RefSeq), at 2,333,947 to 2,335,074 _1960 Shewana3_1961: Shewana3_1961 - hypothetical protein (RefSeq), at 2,335,083 to 2,335,400 _1961 Shewana3_1962: Shewana3_1962 - hypothetical protein (RefSeq), at 2,335,397 to 2,335,678 _1962 Shewana3_1963: ansA - cytoplasmic asparaginase I (RefSeq), at 2,335,749 to 2,336,762 ansA Shewana3_1964: Shewana3_1964 - signal peptide peptidase SppA, 67K type (RefSeq), at 2,336,874 to 2,338,718 _1964 Position (kb) 2335 2336 2337Strain fitness (log2 ratio) -2 -1 0 1at 2334.952 kb on - strand, within Shewana3_1960at 2335.024 kb on + strandat 2335.558 kb on + strand, within Shewana3_1962at 2335.845 kb on - strandat 2335.938 kb on - strand, within ansAat 2336.041 kb on - strand, within ansAat 2336.150 kb on - strand, within ansAat 2336.215 kb on + strand, within ansAat 2336.463 kb on + strandat 2336.541 kb on - strand, within ansAat 2336.760 kb on + strandat 2336.920 kb on - strandat 2336.981 kb on + strandat 2337.126 kb on + strand, within Shewana3_1964at 2337.500 kb on + strand, within Shewana3_1964

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Proline (N)
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2,334,952 - Shewana3_1960 0.89 -2.1
2,335,024 + -0.4
2,335,558 + Shewana3_1962 0.57 +0.2
2,335,845 - -1.4
2,335,938 - ansA Shewana3_1963 0.19 -1.2
2,336,041 - ansA Shewana3_1963 0.29 -0.8
2,336,150 - ansA Shewana3_1963 0.40 -0.1
2,336,215 + ansA Shewana3_1963 0.46 -0.7
2,336,463 + -0.4
2,336,541 - ansA Shewana3_1963 0.78 -1.4
2,336,760 + -2.2
2,336,920 - +0.2
2,336,981 + +1.4
2,337,126 + Shewana3_1964 0.14 +1.4
2,337,500 + Shewana3_1964 0.34 -1.8

Or see this region's nucleotide sequence