Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1721 and Shewana3_1722 are separated by 116 nucleotides Shewana3_1722 and Shewana3_1723 are separated by 15 nucleotides Shewana3_1723 and Shewana3_1724 overlap by 10 nucleotides Shewana3_1724 and Shewana3_1725 are separated by 164 nucleotides
Shewana3_1721: Shewana3_1721 - metal dependent phosphohydrolase (RefSeq), at 2,023,995 to 2,025,257
_1721
Shewana3_1722: Shewana3_1722 - prolyl 4-hydroxylase, alpha subunit (RefSeq), at 2,025,374 to 2,026,069
_1722
Shewana3_1723: Shewana3_1723 - ATPase domain-containing protein (RefSeq), at 2,026,085 to 2,027,434
_1723
Shewana3_1724: Shewana3_1724 - two component transcriptional regulator (RefSeq), at 2,027,425 to 2,028,108
_1724
Shewana3_1725: Shewana3_1725 - hypothetical protein (RefSeq), at 2,028,273 to 2,028,620
_1725
Position (kb)
2026
2027
2028 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2025.560 kb on + strand, within Shewana3_1722 at 2025.614 kb on + strand, within Shewana3_1722 at 2025.873 kb on + strand, within Shewana3_1722 at 2026.333 kb on - strand, within Shewana3_1723 at 2026.338 kb on - strand, within Shewana3_1723 at 2026.538 kb on + strand, within Shewana3_1723 at 2026.875 kb on + strand, within Shewana3_1723 at 2027.063 kb on + strand, within Shewana3_1723 at 2027.472 kb on + strand at 2027.480 kb on - strand at 2027.480 kb on - strand at 2027.531 kb on - strand, within Shewana3_1724 at 2027.724 kb on - strand, within Shewana3_1724 at 2028.118 kb on - strand at 2028.212 kb on - strand at 2028.421 kb on - strand, within Shewana3_1725
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 2,025,560 + Shewana3_1722 0.27 -0.1 2,025,614 + Shewana3_1722 0.34 -1.8 2,025,873 + Shewana3_1722 0.72 +0.4 2,026,333 - Shewana3_1723 0.18 -1.4 2,026,338 - Shewana3_1723 0.19 -0.8 2,026,538 + Shewana3_1723 0.34 +0.0 2,026,875 + Shewana3_1723 0.59 -3.0 2,027,063 + Shewana3_1723 0.72 -1.8 2,027,472 + -2.3 2,027,480 - -0.2 2,027,480 - -3.0 2,027,531 - Shewana3_1724 0.15 -1.0 2,027,724 - Shewana3_1724 0.44 -0.9 2,028,118 - +0.8 2,028,212 - -1.6 2,028,421 - Shewana3_1725 0.43 -1.1
Or see this region's nucleotide sequence