Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1189 and Shewana3_1190 are separated by 169 nucleotides Shewana3_1190 and Shewana3_1191 are separated by 68 nucleotides Shewana3_1191 and Shewana3_1192 overlap by 1 nucleotides Shewana3_1192 and Shewana3_1193 are separated by 75 nucleotides
Shewana3_1189: Shewana3_1189 - RdgB/HAM1 family non-canonical purine NTP pyrophosphatase (RefSeq), at 1,397,981 to 1,398,598
_1189
Shewana3_1190: Shewana3_1190 - hypothetical protein (RefSeq), at 1,398,768 to 1,399,202
_1190
Shewana3_1191: Shewana3_1191 - hypothetical protein (RefSeq), at 1,399,271 to 1,399,561
_1191
Shewana3_1192: Shewana3_1192 - protein of unknown function YGGT (RefSeq), at 1,399,561 to 1,400,109
_1192
Shewana3_1193: Shewana3_1193 - pyrroline-5-carboxylate reductase (RefSeq), at 1,400,185 to 1,401,003
_1193
Position (kb)
1399
1400
1401 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1398.805 kb on + strand at 1399.097 kb on - strand, within Shewana3_1190 at 1399.358 kb on + strand, within Shewana3_1191 at 1399.559 kb on + strand at 1399.735 kb on + strand at 1399.969 kb on - strand, within Shewana3_1192 at 1400.026 kb on + strand, within Shewana3_1192 at 1400.034 kb on - strand, within Shewana3_1192 at 1400.340 kb on + strand, within Shewana3_1193 at 1400.553 kb on - strand, within Shewana3_1193 at 1400.639 kb on - strand, within Shewana3_1193 at 1400.661 kb on + strand, within Shewana3_1193 at 1400.699 kb on + strand at 1400.699 kb on + strand, within Shewana3_1193 at 1400.699 kb on + strand, within Shewana3_1193 at 1400.720 kb on + strand, within Shewana3_1193 at 1400.861 kb on - strand, within Shewana3_1193 at 1400.921 kb on + strand, within Shewana3_1193 at 1400.921 kb on + strand, within Shewana3_1193 at 1400.998 kb on + strand at 1401.022 kb on + strand at 1401.022 kb on + strand at 1401.030 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 1,398,805 + +0.0 1,399,097 - Shewana3_1190 0.76 +1.0 1,399,358 + Shewana3_1191 0.30 +1.0 1,399,559 + -2.1 1,399,735 + +1.3 1,399,969 - Shewana3_1192 0.74 -0.3 1,400,026 + Shewana3_1192 0.85 -0.3 1,400,034 - Shewana3_1192 0.86 -1.3 1,400,340 + Shewana3_1193 0.19 -0.7 1,400,553 - Shewana3_1193 0.45 +0.7 1,400,639 - Shewana3_1193 0.55 +0.5 1,400,661 + Shewana3_1193 0.58 +0.9 1,400,699 + +0.2 1,400,699 + Shewana3_1193 0.63 +1.0 1,400,699 + Shewana3_1193 0.63 -0.3 1,400,720 + Shewana3_1193 0.65 +1.8 1,400,861 - Shewana3_1193 0.83 -0.1 1,400,921 + Shewana3_1193 0.90 +0.3 1,400,921 + Shewana3_1193 0.90 +1.3 1,400,998 + -0.4 1,401,022 + -0.3 1,401,022 + +0.1 1,401,030 - -0.0
Or see this region's nucleotide sequence