Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0622 and Shewana3_0623 are separated by 77 nucleotides Shewana3_0623 and Shewana3_0624 are separated by 77 nucleotides Shewana3_0624 and Shewana3_0625 are separated by 170 nucleotides
Shewana3_0622: Shewana3_0622 - ATP-dependent helicase HrpB (RefSeq), at 728,053 to 730,560
_0622
Shewana3_0623: Shewana3_0623 - penicillin-binding protein 1B (RefSeq), at 730,638 to 732,932
_0623
Shewana3_0624: Shewana3_0624 - PpiC-type peptidyl-prolyl cis-trans isomerase (RefSeq), at 733,010 to 733,288
_0624
Shewana3_0625: Shewana3_0625 - hypothetical protein (RefSeq), at 733,459 to 733,896
_0625
Position (kb) 730
731
732
733 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 729.679 kb on + strand, within Shewana3_0622 at 730.016 kb on - strand, within Shewana3_0622 at 730.234 kb on - strand, within Shewana3_0622 at 730.447 kb on - strand at 730.561 kb on + strand at 730.686 kb on - strand at 730.786 kb on + strand at 730.865 kb on - strand at 730.874 kb on + strand, within Shewana3_0623 at 730.901 kb on - strand, within Shewana3_0623 at 731.037 kb on + strand, within Shewana3_0623 at 731.231 kb on - strand, within Shewana3_0623 at 731.292 kb on - strand, within Shewana3_0623 at 731.299 kb on + strand, within Shewana3_0623 at 731.376 kb on + strand, within Shewana3_0623 at 731.384 kb on - strand, within Shewana3_0623 at 731.455 kb on - strand, within Shewana3_0623 at 731.482 kb on + strand, within Shewana3_0623 at 731.482 kb on + strand, within Shewana3_0623 at 731.482 kb on + strand, within Shewana3_0623 at 731.509 kb on - strand, within Shewana3_0623 at 731.526 kb on + strand, within Shewana3_0623 at 731.594 kb on + strand, within Shewana3_0623 at 731.594 kb on + strand, within Shewana3_0623 at 731.599 kb on + strand, within Shewana3_0623 at 731.607 kb on - strand, within Shewana3_0623 at 731.626 kb on + strand, within Shewana3_0623 at 731.626 kb on + strand, within Shewana3_0623 at 731.660 kb on + strand, within Shewana3_0623 at 731.697 kb on + strand, within Shewana3_0623 at 731.717 kb on + strand, within Shewana3_0623 at 731.778 kb on - strand, within Shewana3_0623 at 731.936 kb on + strand, within Shewana3_0623 at 732.006 kb on + strand, within Shewana3_0623 at 732.428 kb on - strand, within Shewana3_0623 at 732.447 kb on + strand, within Shewana3_0623 at 732.573 kb on + strand, within Shewana3_0623 at 732.709 kb on + strand at 732.865 kb on - strand at 732.877 kb on - strand at 733.173 kb on - strand, within Shewana3_0624 at 733.176 kb on + strand, within Shewana3_0624 at 733.219 kb on + strand, within Shewana3_0624 at 733.304 kb on - strand at 733.438 kb on - strand at 733.503 kb on + strand, within Shewana3_0625 at 733.503 kb on + strand, within Shewana3_0625 at 733.511 kb on - strand, within Shewana3_0625 at 733.511 kb on - strand, within Shewana3_0625 at 733.511 kb on - strand, within Shewana3_0625 at 733.511 kb on - strand, within Shewana3_0625 at 733.511 kb on - strand, within Shewana3_0625 at 733.816 kb on + strand, within Shewana3_0625 at 733.824 kb on - strand, within Shewana3_0625 at 733.831 kb on - strand, within Shewana3_0625 at 733.915 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 729,679 + Shewana3_0622 0.65 +1.2 730,016 - Shewana3_0622 0.78 +0.3 730,234 - Shewana3_0622 0.87 +0.1 730,447 - -0.7 730,561 + +0.1 730,686 - -1.5 730,786 + -1.8 730,865 - -1.3 730,874 + Shewana3_0623 0.10 -1.2 730,901 - Shewana3_0623 0.11 -2.1 731,037 + Shewana3_0623 0.17 -1.6 731,231 - Shewana3_0623 0.26 -1.6 731,292 - Shewana3_0623 0.28 +0.1 731,299 + Shewana3_0623 0.29 -1.3 731,376 + Shewana3_0623 0.32 -2.1 731,384 - Shewana3_0623 0.33 -0.1 731,455 - Shewana3_0623 0.36 +0.2 731,482 + Shewana3_0623 0.37 -1.4 731,482 + Shewana3_0623 0.37 -0.9 731,482 + Shewana3_0623 0.37 -1.8 731,509 - Shewana3_0623 0.38 -1.7 731,526 + Shewana3_0623 0.39 -1.7 731,594 + Shewana3_0623 0.42 -1.3 731,594 + Shewana3_0623 0.42 -0.5 731,599 + Shewana3_0623 0.42 -0.0 731,607 - Shewana3_0623 0.42 -1.6 731,626 + Shewana3_0623 0.43 -1.5 731,626 + Shewana3_0623 0.43 -2.3 731,660 + Shewana3_0623 0.45 -2.8 731,697 + Shewana3_0623 0.46 +0.4 731,717 + Shewana3_0623 0.47 -0.9 731,778 - Shewana3_0623 0.50 -0.5 731,936 + Shewana3_0623 0.57 -2.5 732,006 + Shewana3_0623 0.60 -1.1 732,428 - Shewana3_0623 0.78 -2.3 732,447 + Shewana3_0623 0.79 -2.1 732,573 + Shewana3_0623 0.84 -1.7 732,709 + -0.9 732,865 - -0.6 732,877 - -0.3 733,173 - Shewana3_0624 0.58 -0.3 733,176 + Shewana3_0624 0.59 -0.4 733,219 + Shewana3_0624 0.75 +0.2 733,304 - +0.4 733,438 - +0.2 733,503 + Shewana3_0625 0.10 -0.8 733,503 + Shewana3_0625 0.10 -1.8 733,511 - Shewana3_0625 0.12 +0.6 733,511 - Shewana3_0625 0.12 -1.1 733,511 - Shewana3_0625 0.12 -1.7 733,511 - Shewana3_0625 0.12 +0.3 733,511 - Shewana3_0625 0.12 -0.5 733,816 + Shewana3_0625 0.82 +1.2 733,824 - Shewana3_0625 0.83 -0.1 733,831 - Shewana3_0625 0.85 -1.4 733,915 + +0.1
Or see this region's nucleotide sequence