Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0117 and Shewana3_0118 are separated by 1 nucleotides Shewana3_0118 and Shewana3_0119 are separated by 25 nucleotides Shewana3_0119 and Shewana3_0120 are separated by 129 nucleotides Shewana3_0120 and Shewana3_0121 are separated by 4 nucleotides
Shewana3_0117: Shewana3_0117 - MscS mechanosensitive ion channel (RefSeq), at 132,797 to 134,065
_0117
Shewana3_0118: Shewana3_0118 - lysine exporter protein LysE/YggA (RefSeq), at 134,067 to 134,690
_0118
Shewana3_0119: Shewana3_0119 - phospholipid/glycerol acyltransferase (RefSeq), at 134,716 to 135,276
_0119
Shewana3_0120: Shewana3_0120 - hypothetical protein (RefSeq), at 135,406 to 135,855
_0120
Shewana3_0121: Shewana3_0121 - hypothetical protein (RefSeq), at 135,860 to 136,660
_0121
Position (kb) 134
135
136 Strain fitness (log2 ratio) -1
0
1 at 133.734 kb on - strand, within Shewana3_0117 at 134.087 kb on - strand at 134.097 kb on + strand at 134.193 kb on - strand, within Shewana3_0118 at 134.216 kb on + strand, within Shewana3_0118 at 134.307 kb on + strand, within Shewana3_0118 at 134.307 kb on + strand, within Shewana3_0118 at 134.315 kb on - strand, within Shewana3_0118 at 134.316 kb on - strand, within Shewana3_0118 at 134.564 kb on - strand, within Shewana3_0118 at 134.564 kb on - strand, within Shewana3_0118 at 134.564 kb on - strand, within Shewana3_0118 at 134.569 kb on - strand, within Shewana3_0118 at 134.766 kb on + strand at 134.774 kb on - strand, within Shewana3_0119 at 134.774 kb on - strand, within Shewana3_0119 at 135.591 kb on + strand, within Shewana3_0120 at 135.591 kb on + strand, within Shewana3_0120 at 135.599 kb on - strand, within Shewana3_0120 at 135.693 kb on - strand, within Shewana3_0120 at 135.936 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 133,734 - Shewana3_0117 0.74 +0.0 134,087 - -0.5 134,097 + +0.0 134,193 - Shewana3_0118 0.20 -0.6 134,216 + Shewana3_0118 0.24 +0.4 134,307 + Shewana3_0118 0.38 +0.8 134,307 + Shewana3_0118 0.38 -0.8 134,315 - Shewana3_0118 0.40 +0.6 134,316 - Shewana3_0118 0.40 -0.6 134,564 - Shewana3_0118 0.80 -0.7 134,564 - Shewana3_0118 0.80 -0.1 134,564 - Shewana3_0118 0.80 +0.4 134,569 - Shewana3_0118 0.80 -0.8 134,766 + -0.6 134,774 - Shewana3_0119 0.10 -1.5 134,774 - Shewana3_0119 0.10 -1.2 135,591 + Shewana3_0120 0.41 -0.6 135,591 + Shewana3_0120 0.41 -0.5 135,599 - Shewana3_0120 0.43 +0.7 135,693 - Shewana3_0120 0.64 +0.6 135,936 - +0.7
Or see this region's nucleotide sequence