Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0039 and Shewana3_0040 are separated by 129 nucleotides Shewana3_0040 and Shewana3_0041 are separated by 29 nucleotides Shewana3_0041 and Shewana3_0042 are separated by 30 nucleotides Shewana3_0042 and aroE are separated by 170 nucleotides
Shewana3_0039: Shewana3_0039 - DNA topoisomerase type IA Zn finger domain-containing protein (RefSeq), at 45,802 to 46,365
_0039
Shewana3_0040: Shewana3_0040 - translation factor SUA5 (RefSeq), at 46,495 to 47,061
_0040
Shewana3_0041: Shewana3_0041 - coproporphyrinogen III oxidase (RefSeq), at 47,091 to 47,999
_0041
Shewana3_0042: Shewana3_0042 - globin (RefSeq), at 48,030 to 48,467
_0042
Shewana3_0043: aroE - shikimate 5-dehydrogenase (RefSeq), at 48,638 to 49,516
aroE
Position (kb) 47
48 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 46.106 kb on + strand, within Shewana3_0039 at 46.122 kb on + strand, within Shewana3_0039 at 46.128 kb on + strand, within Shewana3_0039 at 46.130 kb on - strand, within Shewana3_0039 at 46.137 kb on + strand, within Shewana3_0039 at 46.171 kb on - strand, within Shewana3_0039 at 46.368 kb on + strand at 46.409 kb on - strand at 47.079 kb on + strand at 47.166 kb on - strand at 47.241 kb on - strand, within Shewana3_0041 at 47.256 kb on + strand, within Shewana3_0041 at 47.300 kb on + strand, within Shewana3_0041 at 48.161 kb on - strand, within Shewana3_0042 at 48.237 kb on - strand, within Shewana3_0042 at 48.252 kb on - strand, within Shewana3_0042 at 48.292 kb on - strand, within Shewana3_0042 at 48.415 kb on + strand, within Shewana3_0042 at 48.568 kb on + strand at 48.576 kb on - strand at 48.612 kb on - strand at 48.726 kb on + strand, within aroE at 48.742 kb on - strand, within aroE at 48.752 kb on - strand, within aroE at 48.834 kb on - strand, within aroE at 48.858 kb on + strand, within aroE at 48.873 kb on + strand, within aroE at 48.873 kb on + strand, within aroE at 48.881 kb on - strand, within aroE at 48.881 kb on - strand, within aroE at 48.922 kb on + strand, within aroE at 48.922 kb on + strand, within aroE at 48.929 kb on + strand, within aroE at 48.937 kb on - strand, within aroE
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 46,106 + Shewana3_0039 0.54 +0.8 46,122 + Shewana3_0039 0.57 -0.1 46,128 + Shewana3_0039 0.58 +0.5 46,130 - Shewana3_0039 0.58 +0.3 46,137 + Shewana3_0039 0.59 +0.8 46,171 - Shewana3_0039 0.65 +0.2 46,368 + -1.3 46,409 - +0.2 47,079 + -0.6 47,166 - -0.8 47,241 - Shewana3_0041 0.17 -1.2 47,256 + Shewana3_0041 0.18 -0.2 47,300 + Shewana3_0041 0.23 -0.0 48,161 - Shewana3_0042 0.30 +0.9 48,237 - Shewana3_0042 0.47 -1.7 48,252 - Shewana3_0042 0.51 +0.8 48,292 - Shewana3_0042 0.60 -0.3 48,415 + Shewana3_0042 0.88 -0.0 48,568 + +0.4 48,576 - +0.7 48,612 - -2.4 48,726 + aroE Shewana3_0043 0.10 -3.0 48,742 - aroE Shewana3_0043 0.12 -1.7 48,752 - aroE Shewana3_0043 0.13 -0.2 48,834 - aroE Shewana3_0043 0.22 -2.5 48,858 + aroE Shewana3_0043 0.25 -0.6 48,873 + aroE Shewana3_0043 0.27 -3.8 48,873 + aroE Shewana3_0043 0.27 -1.2 48,881 - aroE Shewana3_0043 0.28 -2.4 48,881 - aroE Shewana3_0043 0.28 -5.1 48,922 + aroE Shewana3_0043 0.32 -2.6 48,922 + aroE Shewana3_0043 0.32 -0.8 48,929 + aroE Shewana3_0043 0.33 -3.1 48,937 - aroE Shewana3_0043 0.34 -1.9
Or see this region's nucleotide sequence