Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3354 and Shew_3355 are separated by 80 nucleotides Shew_3355 and Shew_3356 are separated by 774 nucleotides
Shew_3354: Shew_3354 - aminotransferase, class V (RefSeq), at 3,998,747 to 4,001,026
_3354
Shew_3355: Shew_3355 - hypothetical protein (RefSeq), at 4,001,107 to 4,002,327
_3355
Shew_3356: Shew_3356 - CzcA family heavy metal efflux protein (RefSeq), at 4,003,102 to 4,006,332
_3356
Position (kb)
4001
4002
4003 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4000.297 kb on - strand, within Shew_3354 at 4000.305 kb on - strand, within Shew_3354 at 4000.464 kb on + strand, within Shew_3354 at 4000.472 kb on + strand, within Shew_3354 at 4000.480 kb on - strand, within Shew_3354 at 4000.480 kb on - strand, within Shew_3354 at 4000.499 kb on + strand, within Shew_3354 at 4000.993 kb on + strand at 4001.004 kb on + strand at 4001.020 kb on + strand at 4001.160 kb on - strand at 4001.163 kb on + strand at 4001.171 kb on - strand at 4001.447 kb on - strand, within Shew_3355 at 4001.600 kb on - strand, within Shew_3355 at 4001.600 kb on - strand, within Shew_3355 at 4001.600 kb on - strand, within Shew_3355 at 4001.672 kb on + strand, within Shew_3355 at 4001.721 kb on + strand, within Shew_3355 at 4001.916 kb on - strand, within Shew_3355 at 4001.942 kb on + strand, within Shew_3355 at 4001.942 kb on + strand, within Shew_3355 at 4002.027 kb on - strand, within Shew_3355 at 4002.126 kb on + strand, within Shew_3355 at 4002.302 kb on - strand at 4002.850 kb on + strand at 4002.965 kb on - strand at 4003.112 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 4,000,297 - Shew_3354 0.68 -0.0 4,000,305 - Shew_3354 0.68 +1.1 4,000,464 + Shew_3354 0.75 +0.3 4,000,472 + Shew_3354 0.76 +0.0 4,000,480 - Shew_3354 0.76 +0.8 4,000,480 - Shew_3354 0.76 -2.2 4,000,499 + Shew_3354 0.77 +0.1 4,000,993 + +0.6 4,001,004 + +1.8 4,001,020 + +0.0 4,001,160 - -0.0 4,001,163 + -0.3 4,001,171 - -0.4 4,001,447 - Shew_3355 0.28 -0.4 4,001,600 - Shew_3355 0.40 +0.2 4,001,600 - Shew_3355 0.40 +0.0 4,001,600 - Shew_3355 0.40 -0.7 4,001,672 + Shew_3355 0.46 +0.2 4,001,721 + Shew_3355 0.50 -0.2 4,001,916 - Shew_3355 0.66 -0.7 4,001,942 + Shew_3355 0.68 -0.3 4,001,942 + Shew_3355 0.68 +0.2 4,002,027 - Shew_3355 0.75 +0.2 4,002,126 + Shew_3355 0.83 -1.0 4,002,302 - -0.6 4,002,850 + -0.1 4,002,965 - -0.1 4,003,112 - -0.6
Or see this region's nucleotide sequence