Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1660 and fur are separated by 90 nucleotides fur and Shew_1662 are separated by 310 nucleotides Shew_1662 and Shew_1663 are separated by 57 nucleotides Shew_1663 and Shew_1664 are separated by 40 nucleotides
Shew_1660: Shew_1660 - GCN5-related N-acetyltransferase (RefSeq), at 1,910,434 to 1,910,934
_1660
Shew_1661: fur - ferric uptake regulator (RefSeq), at 1,911,025 to 1,911,456
fur
Shew_1662: Shew_1662 - NAD-dependent deacetylase (RefSeq), at 1,911,767 to 1,912,513
_1662
Shew_1663: Shew_1663 - hypothetical protein (RefSeq), at 1,912,571 to 1,913,092
_1663
Shew_1664: Shew_1664 - hypothetical protein (RefSeq), at 1,913,133 to 1,913,420
_1664
Position (kb) 1911
1912
1913 Strain fitness (log2 ratio) -1
0
1 at 1910.926 kb on + strand at 1911.080 kb on - strand, within fur at 1912.172 kb on - strand, within Shew_1662 at 1912.967 kb on - strand, within Shew_1663 at 1912.967 kb on - strand, within Shew_1663 at 1913.052 kb on + strand at 1913.086 kb on + strand at 1913.086 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,910,926 + +0.3 1,911,080 - fur Shew_1661 0.13 +0.2 1,912,172 - Shew_1662 0.54 -1.1 1,912,967 - Shew_1663 0.76 -0.9 1,912,967 - Shew_1663 0.76 -1.4 1,913,052 + +0.6 1,913,086 + -0.1 1,913,086 + +0.2
Or see this region's nucleotide sequence