Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt engA and Shew_1295 are separated by 190 nucleotides Shew_1295 and xseA are separated by 231 nucleotides xseA and Shew_1297 are separated by 119 nucleotides
Shew_1294: engA - GTP-binding protein EngA (RefSeq), at 1,494,671 to 1,496,140
engA
Shew_1295: Shew_1295 - hypothetical protein (RefSeq), at 1,496,331 to 1,496,864
_1295
Shew_1296: xseA - exodeoxyribonuclease VII large subunit (RefSeq), at 1,497,096 to 1,498,424
xseA
Shew_1297: Shew_1297 - inositol-5-monophosphate dehydrogenase (RefSeq), at 1,498,544 to 1,500,010
_1297
Position (kb)
1497
1498
1499 Strain fitness (log2 ratio)
-1
0
1 at 1496.197 kb on - strand at 1496.314 kb on + strand at 1496.578 kb on - strand, within Shew_1295 at 1496.784 kb on - strand, within Shew_1295 at 1496.791 kb on - strand, within Shew_1295 at 1497.011 kb on + strand at 1497.101 kb on - strand at 1497.131 kb on + strand at 1497.139 kb on - strand at 1497.161 kb on + strand at 1497.174 kb on + strand at 1497.203 kb on - strand at 1497.294 kb on - strand, within xseA at 1497.315 kb on - strand, within xseA at 1497.388 kb on - strand, within xseA at 1497.428 kb on + strand, within xseA at 1497.718 kb on - strand, within xseA at 1497.755 kb on - strand, within xseA at 1497.762 kb on + strand, within xseA at 1497.964 kb on + strand, within xseA at 1498.208 kb on + strand, within xseA at 1498.347 kb on - strand at 1498.349 kb on + strand at 1498.487 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,496,197 - +0.9 1,496,314 + +0.2 1,496,578 - Shew_1295 0.46 +1.3 1,496,784 - Shew_1295 0.85 +0.1 1,496,791 - Shew_1295 0.86 -0.2 1,497,011 + -0.7 1,497,101 - +0.3 1,497,131 + -1.1 1,497,139 - -1.0 1,497,161 + -0.5 1,497,174 + -0.8 1,497,203 - -0.5 1,497,294 - xseA Shew_1296 0.15 +0.7 1,497,315 - xseA Shew_1296 0.16 -0.6 1,497,388 - xseA Shew_1296 0.22 -0.6 1,497,428 + xseA Shew_1296 0.25 -1.2 1,497,718 - xseA Shew_1296 0.47 -0.3 1,497,755 - xseA Shew_1296 0.50 -0.1 1,497,762 + xseA Shew_1296 0.50 -0.8 1,497,964 + xseA Shew_1296 0.65 -0.8 1,498,208 + xseA Shew_1296 0.84 -0.9 1,498,347 - -0.5 1,498,349 + +0.6 1,498,487 + -0.8
Or see this region's nucleotide sequence