Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1287 and Shew_1288 are separated by 32 nucleotides Shew_1288 and Shew_1289 overlap by 1 nucleotides Shew_1289 and ispG are separated by 19 nucleotides
Shew_1287: Shew_1287 - hypothetical protein (RefSeq), at 1,487,227 to 1,488,348
_1287
Shew_1288: Shew_1288 - type IV pilus biogenesis/stability protein PilW (RefSeq), at 1,488,381 to 1,489,169
_1288
Shew_1289: Shew_1289 - hypothetical protein (RefSeq), at 1,489,169 to 1,490,179
_1289
Shew_1290: ispG - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (RefSeq), at 1,490,199 to 1,491,314
ispG
Position (kb) 1489
1490
1491 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1488.474 kb on + strand, within Shew_1288 at 1489.069 kb on + strand, within Shew_1288 at 1489.156 kb on - strand at 1489.426 kb on + strand, within Shew_1289 at 1489.593 kb on + strand, within Shew_1289 at 1489.800 kb on - strand, within Shew_1289 at 1489.808 kb on - strand, within Shew_1289 at 1489.928 kb on - strand, within Shew_1289
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,488,474 + Shew_1288 0.12 -0.3 1,489,069 + Shew_1288 0.87 +1.3 1,489,156 - -2.8 1,489,426 + Shew_1289 0.25 -2.9 1,489,593 + Shew_1289 0.42 -1.9 1,489,800 - Shew_1289 0.62 +0.2 1,489,808 - Shew_1289 0.63 +0.1 1,489,928 - Shew_1289 0.75 +0.5
Or see this region's nucleotide sequence